Physiological alteration surrounding blood flow in a segmental pancreatic graft-1. the effect of a distal splenic arterio-venous fistula on blood flow, and 2. correlation between prostaglandins and blood flow.

节段性胰腺移植物中血流的生理变化-1。

• 批准号: 63570637
• 负责人: TAMURA Katsuhiro
• 金额: $ 1.34万
• 依托单位: Shimane Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570637/
• 关键词: Segmental pancreatic graft; Splenic arterial blood flow; Tissue blood flow; Distal splenic arteriovenous fistula; Prostaglandin I2; Thromboxane A2; Preservation time; OKY-046; 移植膵血管血流; 移植膵組織血流; PGI_2; TxA_2; 膵グラフト血流; プロスタグランディンI_2; トロンボキサンA_

1. We investigated the effect of a distal splenic arterio-venous fistula (A-V fistula) on splenic arterial blood flow (SABF) and tissue blood flow (TBF) in a segmental pancreatic autograft both in dogs and in clinical cases, and following conclusions were obtained, (1) After splenectomy, not only SABF but also TBF extremely decreased, which phenomena should result in causing thrombosis of the graft. (2) Both SABF and TBF were significantly increased by performing A-V fistula. (3) We applied A-V fistula in 8 clinical cases, which was very effective to prevent thrombosis and to maintain normal pancreatic function of the autograft in all cases.2. A pancreatic graft is generally transplanted heterotopiccally, in other words completely denervated. Then, the blood flow must be mainly regulated byhumoral substances. we investigated. correlation between prostaglafidins (PGI_2, TxA_2) and blood flow, and following conclusions were obtained. (1) Both PGI_2 and TxA_2 were secreted from the graft into the simple cold storage solution, and these secretion continued actively till the first 7 hours in preservation. (2) We divided experimental animals into two groups, the one with autograft preserved for 3 hours (3) hrs group and another with autograft preserved for 24 hours (24 hrs group). In 3 hrs groupSIGMA PGI_2 secretion during preservation was dominant, on the other hand in 24 hrs group SIGMA TxA_2 secretion was dominant. (3) After transplantation, both SABF and TBF were significantly higher in 3 hrs group than in situ denervated control group (so called reactive hyperemia), on the other hand they were remarkably lower in 24 hrs group than in control group. PGI_2/TxA_2 ratio was positively correlative to SABF and TBF. (4) Graft survival rate was 80% in 3 hrs group, and 20% in 24 hrs group. (5) TxA_2 synthetic inhibitor (OKY-046) was effective to elevate PGI_2/TxA_2 ratio, resulting in improvement of SABF and TBF.

1.本文观察了远端脾动静脉瘘（A-V瘘）对自体节段胰腺移植物脾动脉血流量（SABF）和组织血流量（TBF）的影响。结果表明：（1）脾切除后，SABF和TBF均显著降低，这可能是导致移植物血栓形成的原因。(2)动静脉内瘘术后SABF和TBF均显著增加。(3)结论：1.临床应用动静脉内瘘8例，均能有效防止血栓形成，维持移植胰腺功能正常.胰腺移植通常是异位移植，换句话说，完全去神经。因此，血液流动必须主要由体液物质调节。我们调查过了结果表明：血浆PGI_2、TxA_2与血流量呈负相关。(1)PGI_2和TxA_2均从移植物中分泌到单纯冷藏液中，并持续活跃至保存的最初7小时。(2)将实验动物分为两组，一组为自体移植物保存3小时（3 h）组，另一组为自体移植物保存24小时（24 h）组。保存3 h组以SIGMA PGI_2分泌为主，24 h组以SIGMA TxA_2分泌为主。(3)移植后3 h组SABF和TBF均显著高于原位失神经对照组（反应性充血），24 h组SABF和TBF均显著低于原位失神经对照组。PGI_2/TxA_2比值与SABF、TBF呈正相关。(4)移植物存活率3 h组为80%，24 h组为20%。(5)TXA_2合成抑制剂（OKY-046）可提高PGI_2/TXA_2比值，改善SABF和TBF。

项目成果

金聲根: "部分膵移植における遠位脾動静脈fistulaの膵組織血流量に及ぼす影響について-特に脾動脈血流と関連して-" 移植. 23. 365-374 (1988)

金涅根：“论部分胰腺移植中远端脾动静脉瘘对胰腺组织血流的影响——特别是与脾动脉血流的关系——”移植。

G.Kaneko: "Comarison of Graft Survival Between Orthotopic and Heterotopic Segmental Pancreas Allotransplantation." Transplantation Proceedings. 21. 2806-2808 (1989)

G.Kaneko：“原位和异位节段性胰腺同种异体移植之间移植物存活率的比较。”

長見晴彦: "イヌ部分膵自家移植片のプロスタグランジン産生動態と血流量との関連について-特に保存時間との関連について-" 移植. 25. (1990)

Haruhiko Nagami：“关于犬部分胰腺自体移植物中前列腺素产生动态和血流之间的关系 - 特别是与储存时间的关系”25。（1990）。

Katsuhiro Tamura, Atsushi Naitoh, Haruhiko Nagami, Seikon Kin, Keiji Ono, Toru Ishida, Makoto Taramoto, Yasushi Inoue, Tsuyoshi Yamamoto and Akira Nakase: "A case of resection of the body and the tail of the pancreas and ex vivo trimming of the distal pan

Katsuhiro Tamura、Atsushi Naitoh、Haruhiko Nagami、Seikon Kin、Keiji Ono、Toru Ishida、Makoto Taramoto、Yasushi Inoue、Tsuyoshi Yamamoto 和 Akira Nakase：“胰体和尾部切除以及胰体离体修剪的案例

Kin S.: "The effect of a distal splenic arteriovenous fistula on tissue blood flow in the pancreatic segment." Transpl.Proc.21. 2812-2814 (1989)

Kin S.：“远端脾动静脉瘘对胰段组织血流的影响。”