Bioassay of pollution gas by metabolic heat measurement of yeast

酵母代谢热测定对污染气体的生物测定

基本信息

批准号：
13680629
负责人：
TAMURA Katsuhiro
金额：
$ 2.37万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

Using a microcalorimeter the heat evolved during incubation of yeast cultures at 30℃ was detected in the form of growth thermogram (metabolic heat-incubation time curve). Correlation of the heat evolution curves with the number of cells and the turbidity of the culture was found to be very good. In this study, the effects of high-pressure gas in open system on the growth curve of yeast were investigated and the inhibitory action of the gases (nitrogen, air, oxygen, nitrous oxide, argon and krypton) on the growth was quantitatively assayed using a Biothermo Analyzer. The growth thermograms were used to estimate microbial activity of yeast under compressed gases and to determine the 50% inhibitory pressure (IP5O) and minimum inhibitory pressure (MIP) that is regarded as an index of toxic potency of dissolved gases. It was found that the increase of pressure induced clear inhibitory action on yeast cells, especially for The order of the action was as follows O_2>N_2O>air>Kr>N_2>Ar.oxygen.These results can be applied for a bioassay of various toxic and environmental pollution gases such as NH_3, Cl_2, NOx and SOx. The bioassay is the method to determine the quantity of toxic chemicals using biological materials, microorganisms, plants, fishes, shellfish and mammals. Recently, this assay became to be often used in the field of assessment of environmental pollution, however, gas materials were out of this element, especially when microorganisms are used as biological materials, for the low solubilities of gases into water. The method we proposed settled these problems and can be used for the bioassay of gases.

使用微晶光仪以30°孵育期间在30°孵育过程中进化的热量以生长热图的形式（代谢热孵育时间曲线）。发现热进化曲线与细胞数量和培养的浊度的相关性非常好。在这项研究中，研究了高压气体在开放系统中对酵母生长曲线的影响，并使用生物疗法分析仪定量地分配了气体（氮，空气，氧，氧化物，氧化二氮，氧化二氮，氧化二氮，Argrypton）的抑制作用。使用生长热图用于估计压缩气体下酵母的微生物活性，并确定50％的抑制压力（IP5O）和最小抑制压力（MIP），这些压力（MIP）被认为是溶解气体有毒效力的指数。发现压力的增加引起了对酵母细胞的明确抑制作用，尤其是在作用的顺序上如下。 O_2> n_2o>空气> kr> n_2> ar.oxygen。这些结果可以用于各种有毒和环境污染气的生物测定，例如NH_3，CL_2，NOX，NOX和SOX。生物测定是使用生物学材料，微生物，植物，鱼类，贝类和哺乳动物确定有毒化学物质数量的方法。最近，该测定法经常用于环境污染的评估领域，但是，气体材料不超出该元素，尤其是当微生物用作生物学材料时，气体对水的溶解度的低溶解度。我们提出的方法解决了这些问题，可用于气体生物测定。