Enhancement of Alcohol Tolerance of Yeast Applying Pressure Stress of Cell and Pressure Reversal of Anesthesia

细胞压力和麻醉压力逆转增强酵母的酒精耐受性

基本信息

批准号：
04808045
负责人：
TAMURA Katsuhiro
金额：
$ 1.02万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

In the presence of relatively low concentrations of ethanol, small aquatic animals such as tadpoles sink to the bottom of a water tank, but they begin to swim when a pressure of about 20 Mpa is applied. This phenomenon of recovery from an anesthetized state on application of pressure is known as pressure reversal of anesthesia and is the reason why pressure is often used to investigate the mechanisms of action of anesthetics. This phenomenon has also been observed with some microorganisms. On application of pressure to suspensions of E.coli containing a few per cent of ethanol, survival of the cells increases with increase in pressure up to 10-20 Mpa.Yeast produces ethanol from glucose and this product should act an anesthetic on the cells. Accordingly, the growth of yeast should gradually be retarded and some cells should die with the progress of fermentation. If pressure is applied to this system, survival of yeast should be greater than at atmospheric pressure. Therefore, as an application of this phenomenon, we examined the pressure reversal for survival of yeast and examined the fermentation of glucose by yeast at high pressure.Pressure revesal of anesthsia was applied to alcoholic fermentation of glucose by yeast. The survival of yeast in the absence of ethanol decreased with increase in pressure. However, in medium containing 4 and 8%(w/w)ethanol, cell survivals reached peaks at 20 MPa(200 bar). Although viable yeast cells apparently increased at a pressure of 20 MPa, no increase of the alcohol concentration was observed under the experimental conditions used.

在乙醇浓度相对较低的情况下，小型水生动物（例如t）下沉到水箱的底部，但是当施加约20 MPa的压力时，它们开始游泳。从麻醉状态恢复压力时，这种现象称为麻醉的压力逆转，这是压力通常用于研究麻醉剂作用机理的原因。一些微生物也观察到了这种现象。在将压力施加到含有几个乙醇的大肠杆菌的悬浮液中时，细胞的存活随压力增加而增加，最高为10-20 mpa.yeast会产生葡萄糖的乙醇，该产物应在细胞上起麻醉性。因此，酵母的生长应逐渐阻碍，并且某些细胞应随着发酵的进展而死。如果向该系统施加压力，则酵母的存活应大于在大气压下的生存。因此，作为这种现象的应用，我们检查了酵母生存的压力逆转，并检查了酵母在高压下通过酵母发酵的葡萄糖。在没有乙醇的情况下，酵母的存活随压力增加而降低。但是，在包含4％和8％（W/W）乙醇的培养基中，细胞存活率以20 MPa（200 Bar）达到峰值。尽管在20 MPa的压力下显然有活跃的酵母细胞升高，但在所使用的实验条件下未观察到酒精浓度的增加。