Physiological roles of growth factors in mammalian reproduction.

生长因子在哺乳动物繁殖中的生理作用。

基本信息

批准号：
63570771
负责人：
TSUTSUMI Osamu
金额：
$ 1.41万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570771/
关键词：
Growth factor Epidermal growth factor EGF Gamete Sperm Oocyte Embryo Fetus EGF Epidermal growth factor(EGF)卵子宮流産

项目摘要

Physiological roles of growth factors especially epidermal growth factor (EGF) were studied in the field of mammalian reproduction and major findings are as follows :I. Establishment of pregnancy and fetal growth.Plasma EGF concentrations were elevated during pregnancy in human and there was positive correlation between maternal and fetal EGF levels although fetal levels were significantly lower than maternal ones.Sialoadenectomy (removal of the submandibular glands) of pregnant mice was shown to cause EGF deficiency during pregnancy which resulted in intrauterine growth retardation (IUGR). This makes an animal model to study the mechanism of fetal growth and IUGR.II. Maturation of oocyte and spermatozoa.EGFlike substance (EGF-L) was present in the human follicular fluid obtained from patients in our in vitro fertilization program. The concentration of EGF-L was related with the maturity of oocyte within. EGF-L was also present in human seminal fluid and concerned with sperm morbidity.EGF stimulated the maturation of rat oocyte in culture in terms of both germinal vesicle breakdown (resumption of meiosis) and phosphofructokinase activity (rate-limiting enzyme present in the cytoplasma).III. Development of preimplantation embryo.Presence and number of EGF receptor was determined using binding assay with I-125 EGF. Presence of EGF receptor was demonstrated in mouse oocyte and the number of EGF receptor was increased as embryos developed in preimplantation period. EGF added to the culture medium stimulated the development of 2-cell embryos.

在哺乳动物繁殖领域研究了生长因子的生理作用，特别是表皮生长因子（EGF），主要发现如下：i。妊娠和胎儿生长的建立。质量EGF浓度在人类怀孕期间升高，尽管胎儿水平明显低于母体，但在孕产妇和胎儿EGF水平之间存在正相关。在孕期会导致EGF的增长（在孕期间，孕妇的孕妇缺乏症状），表现出孕妇的缺乏症。这使动物模型研究了胎儿生长和IUGR.II的机制。卵母细胞和精子物质的成熟（EGF-L）存在于我们体外受精计划中的患者的人卵泡液中。 EGF-L的浓度与卵母细胞的成熟度有关。 EGF-L也存在于人类的精液中，并且与精子发病有关。EGF在生发囊泡分解（减数分裂恢复）和磷酸果果酶动物酶活性（胞质中存在的速率限制酶）的培养物中刺激了大鼠卵母细胞在培养中的成熟。使用I-125 EGF的结合测定确定植入前胚胎的发展和EGF受体的数量。在小鼠卵母细胞中证明了EGF受体的存在，并且随着植入前的胚胎发育而增加EGF受体的数量。 EGF添加到培养基中刺激了2细胞胚胎的发展。