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Physiological roles of growth factors in mammalian reproduction.

生长因子在哺乳动物繁殖中的生理作用。

基本信息

批准号：
63570771
负责人：
TSUTSUMI Osamu
金额：
$ 1.41万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570771/
关键词：
Growth factor Epidermal growth factor EGF Gamete Sperm Oocyte Embryo Fetus EGF Epidermal growth factor(EGF)卵子宮流産

项目摘要

Physiological roles of growth factors especially epidermal growth factor (EGF) were studied in the field of mammalian reproduction and major findings are as follows :I. Establishment of pregnancy and fetal growth.Plasma EGF concentrations were elevated during pregnancy in human and there was positive correlation between maternal and fetal EGF levels although fetal levels were significantly lower than maternal ones.Sialoadenectomy (removal of the submandibular glands) of pregnant mice was shown to cause EGF deficiency during pregnancy which resulted in intrauterine growth retardation (IUGR). This makes an animal model to study the mechanism of fetal growth and IUGR.II. Maturation of oocyte and spermatozoa.EGFlike substance (EGF-L) was present in the human follicular fluid obtained from patients in our in vitro fertilization program. The concentration of EGF-L was related with the maturity of oocyte within. EGF-L was also present in human seminal fluid and concerned with sperm morbidity.EGF stimulated the maturation of rat oocyte in culture in terms of both germinal vesicle breakdown (resumption of meiosis) and phosphofructokinase activity (rate-limiting enzyme present in the cytoplasma).III. Development of preimplantation embryo.Presence and number of EGF receptor was determined using binding assay with I-125 EGF. Presence of EGF receptor was demonstrated in mouse oocyte and the number of EGF receptor was increased as embryos developed in preimplantation period. EGF added to the culture medium stimulated the development of 2-cell embryos.

生长因子尤其是表皮生长因子（EGF）在哺乳动物生殖过程中的生理作用已被广泛研究。妊娠的建立和胎儿的生长。人类妊娠期间血浆EGF浓度升高，母体和胎儿EGF水平之间存在正相关，尽管胎儿EGF水平显著低于母体。妊娠小鼠的涎腺切除术（切除下颌下腺）被证明会导致妊娠期间EGF缺乏，从而导致宫内生长迟缓（IUGR）。这为研究胎儿生长发育和IUGR机制提供了一种动物模型。卵母细胞和精子的成熟：在我们的体外受精程序中，从患者的卵泡液中获得了EGF样物质（EGF-L）。EGF-L的浓度与卵母细胞的成熟度有关。人精液中也含有EGF-L，与精子的发病率有关。EGF刺激培养的大鼠卵母细胞的成熟，包括成熟囊泡破裂（减数分裂的恢复）和磷酸果糖激酶活性（细胞质中存在的限速酶）。植入前胚胎的发育：用I-125 EGF结合试验测定EGF受体的存在和数量。EGF受体在小鼠卵母细胞中存在，并随胚胎发育而增加。加入EGF的培养液刺激2-细胞胚胎的发育。