Analysis of the microenvironment and cryopreservation of embryos on their development
胚胎发育的微环境和冷冻保存分析
基本信息
- 批准号:08671870
- 负责人:
- 金额:$ 1.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Microtechniques and highly sensitive methods such as enzymatic cycling, micro-Western analysis, reverse transcription polymerase chain reaction and so on were employed to study these processes. Low hexokinase activity and high activities of enzymes in the phosphate pathway were characteristic of immature oocytes. During maturation, the activities of hexokinase and phosphofructokinase increased significantly. These changes were used to analyze involvement of epidermal growth factor (EGF) and prostaglandins (PG) in oocyte maturation EGF is shown to stimulate maturation by increasing PG production in granulosa cells. Electro-physiologically, the sensitivity of oocyte to inositol triphosphate increased and Ca^<2+> release system developed during maturation. Progesterone production of oocyte and embryos are shown by enzymatic cycling and other methods using radiometry. This hormone produced by embryos themselves may play a role in embryonic development in intracrine fashion. There is 100-fold increase in glucose uptake from oocyte to blastocyst in mice. A switch in substrate preferende of the embryo from pyruvate to glycose during preimplantation development may be explained by increases in the activity of hexokinase and expression of glycose transporter, GLUT1. Hexokinase activities determined by NADP cycling increased 20-fold while expression of GLUT1 assessed by micro-Western method 10-fold. GLUT1 expression was also analyzed by RT-PCR,which indicated that the expression is regulated at transcription level. There is a delay in the develomental changes in glucose uptake, hexokinase activity and GLUT1 expression when the embryos are developed in vitro. It is of interest to note that EGF stimulates these developmental changes in vitro. In conclusion, expanding knowledge in the process of oocyte maturation and embryonic development may serve not only as significant clinical implications for in vitro fertilization techniques but as breakthroughs in gynecology.
利用酶循环、微量Western分析、逆转录聚合酶链反应等微量技术和高灵敏度的方法对这些过程进行了研究。低己糖激酶活性和高活性的酶在磷酸途径是未成熟卵母细胞的特征。成熟过程中,己糖激酶和磷酸果糖激酶活性显着增加。这些变化被用来分析表皮生长因子(EGF)和前列腺素(PG)在卵母细胞成熟中的参与。EGF显示通过增加颗粒细胞中PG的产生来刺激成熟。在电生理上,卵母细胞成熟过程中对三磷酸肌醇的敏感性增加,Ca^2+释放系统发育。卵母细胞和胚胎的孕激素产生通过酶循环和其他使用辐射测量的方法来显示。这种由胚胎自身产生的激素可能以内分泌的方式在胚胎发育中发挥作用。小鼠从卵母细胞到胚泡的葡萄糖摄取增加了100倍。胚胎在着床前发育过程中对底物的偏好从丙酮酸转变为葡萄糖,这可以通过增加己糖激酶的活性和葡萄糖转运蛋白GLUT 1的表达来解释。通过NADP循环测定的己糖激酶活性增加20倍,而通过微量蛋白质方法评估的GLUT 1表达增加10倍。RT-PCR分析GLUT 1的表达,结果表明GLUT 1的表达在转录水平上受到调控。胚胎在体外发育过程中,葡萄糖摄取、己糖激酶活性和GLUT 1表达的发育变化延迟。值得注意的是,EGF在体外刺激这些发育变化。总之,扩大卵母细胞成熟和胚胎发育过程中的知识,不仅可以作为体外受精技术的重要临床意义,但在妇科学的突破。
项目成果
期刊论文数量(19)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
jinbo H, Hitomi Y, Tsutsumi O, et al: "Evidence for monoclonal expansionof epithelial cells in ovarian endometr-ial cysts." American Journal of Pathology. 150. 1173-1178 (1997)
jinbo H、Hitomi Y、Ttsutsumi O 等人:“卵巢子宫内膜囊肿中上皮细胞单克隆扩增的证据。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
堤 治 他: "腹腔鏡下手術" メジカルビュー社, 191 (1996)
Osamu Tsutsumi 等人:《腹腔镜手术》Medical View Publishing,191 (1996)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Tsutsumi O, Morita Y, Momoeda M, Ya-no T, Taketani Y.: "Factors influencing embryo quali-ty and viability in preimplantation Per-iod-glucose incorporation can be a meas-ure of embryo quality and viability,IN in vitro fertilization and assisted rep-roducti
Tsutsumi O、Morita Y、Momoeda M、Ya-no T、Taketani Y.:“影响植入前胚胎质量和活力的因素。碘期葡萄糖掺入可以作为胚胎质量和活力的衡量标准,在体外
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Tsutsumi O,Yoshimura Y: "Sex Differentiation and Regulation of Ovarian Function : An Overview" Hormone Research. 46(S1). 1-5 (1996)
Tsutsumi O,Yoshimura Y:“卵巢功能的性别分化和调节:概述”激素研究。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Matsumi H, et al: "Expression and localization of inducible nitric oxide synthase in the rat ovary:a possible involvement of nitric oxide in the follicular development." Biochemical and Biophysical Research Communications.(in press).
Matsumi H 等人:“诱导型一氧化氮合酶在大鼠卵巢中的表达和定位:一氧化氮可能参与卵泡发育。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
TSUTSUMI Osamu其他文献
TSUTSUMI Osamu的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('TSUTSUMI Osamu', 18)}}的其他基金
White-color luminescence from a single material with harmony between order- and disorder-structures of luminogen aggregates
单一材料的白色发光,发光体聚集体的有序结构和无序结构之间的协调
- 批准号:
15K05613 - 财政年份:2015
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Self-organization of gold nanoparticles and photochemical manipulation of their organized structures by means of liquid-crystalline molecules
金纳米粒子的自组织及其通过液晶分子对其组织结构进行光化学操纵
- 批准号:
21710094 - 财政年份:2009
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Development of Photoresponsive Organic-Inorganic Hybrid Liquid Crystals : Spontaneous Organization of Inorganic Nanoparticles with Organic Molecules
光响应有机-无机杂化液晶的开发:无机纳米粒子与有机分子的自发组织
- 批准号:
19559003 - 财政年份:2007
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
A laboratory and clinical study of the mechanism of mother-to-child infection of Toxoplasma
弓形虫母婴感染机制的实验室及临床研究
- 批准号:
15591732 - 财政年份:2003
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
STUDIES ON FACTORS RELATED TO EMBRYO DEVELOPMENT TO IMPROVE OUTCOME OF ASSITED REPRODUCTIVE TECHNOLOGY
研究胚胎发育相关因素以提高辅助生殖技术的效果
- 批准号:
12671580 - 财政年份:2000
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
BASIC STUDIES FOR IMPROVEMENT OF IN VITRO FERTILIZATION AND CRYOPRESERVATION
改进体外受精和冷冻保存的基础研究
- 批准号:
10671518 - 财政年份:1998
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Assessmemt of embryo viability and its microenvironment
胚胎活力及其微环境的评估
- 批准号:
06671632 - 财政年份:1994
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Biochemical and endocrinological analysis of oocytes and embryos
卵母细胞和胚胎的生化和内分泌分析
- 批准号:
03670771 - 财政年份:1991
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Physiological roles of growth factors in mammalian reproduction.
生长因子在哺乳动物繁殖中的生理作用。
- 批准号:
63570771 - 财政年份:1988
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似国自然基金
"胚胎/生殖细胞发育特性激活”促进“神经胶质瘤恶变”的机制及其临床价值研究
- 批准号:82372327
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
酪氨酸激酶Pyk2对小鼠着床前胚胎细胞增殖和存活的影响
- 批准号:31101034
- 批准年份:2011
- 资助金额:20.0 万元
- 项目类别:青年科学基金项目
相似海外基金
The Role of Uterine Glycogen in Establishing a Successful Pregnancy
子宫糖原在成功怀孕中的作用
- 批准号:
10725894 - 财政年份:2023
- 资助金额:
$ 1.41万 - 项目类别:
Label-free single-cell imaging for quality control of cardiomyocyte biomanufacturing
用于心肌细胞生物制造质量控制的无标记单细胞成像
- 批准号:
10675976 - 财政年份:2023
- 资助金额:
$ 1.41万 - 项目类别:
Yolk Sac MicroRNAs Regulate Brain Development
卵黄囊 MicroRNA 调节大脑发育
- 批准号:
10750884 - 财政年份:2023
- 资助金额:
$ 1.41万 - 项目类别:
Altered metabolism in embryo generated by in vitro fertilization
体外受精产生的胚胎代谢发生改变
- 批准号:
10583414 - 财政年份:2023
- 资助金额:
$ 1.41万 - 项目类别:
Development of approaches for inducible trophoblast-specific gene modulation: the role of trophoblast Lat1 in the regulation of placental function and fetal growth
开发诱导性滋养层特异性基因调节方法:滋养层 Lat1 在胎盘功能和胎儿生长调节中的作用
- 批准号:
10656023 - 财政年份:2023
- 资助金额:
$ 1.41万 - 项目类别:
Endothelial Regulation of Astrocyte Trans-differentiation in Stroke
中风中星形胶质细胞转分化的内皮调节
- 批准号:
10712315 - 财政年份:2023
- 资助金额:
$ 1.41万 - 项目类别:
Fueling left-right asymmetry: the role of glycolysis in stomach curvature
加剧左右不对称:糖酵解在胃曲率中的作用
- 批准号:
10605914 - 财政年份:2023
- 资助金额:
$ 1.41万 - 项目类别:
Decoding the signature of sperm RNA & RNA modification of environmental stressors on the intergenerational transmission of metabolic phenotypes
解码精子 RNA 的特征
- 批准号:
10869714 - 财政年份:2023
- 资助金额:
$ 1.41万 - 项目类别:
Role of trefoil factor family proteins in beta cell function.
三叶因子家族蛋白在β细胞功能中的作用。
- 批准号:
10566731 - 财政年份:2023
- 资助金额:
$ 1.41万 - 项目类别:
Cell signaling and transcription factors in pancreatic islet development and function
胰岛发育和功能中的细胞信号传导和转录因子
- 批准号:
10783216 - 财政年份:2023
- 资助金额:
$ 1.41万 - 项目类别: