A laboratory and clinical study of the mechanism of mother-to-child infection of Toxoplasma

弓形虫母婴感染机制的实验室及临床研究

• 批准号: 15591732
• 负责人: TSUTSUMI Osamu
• 金额: $ 2.24万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591732/
• 关键词: Toxoplasma; avidity; anti-Toxoplasma IgM antibody; mother-to-child transmission; congenital toxoplasmosis; murine model; per oral infection; small intestine

ObjectivesCongenital Toxoplasma infection in the fetus frequently results in debilitating disease in the newborn. To better evaluate the period of maternal infection, we examined avidity of maternal anti-Toxoplasma IgG.Study MethodsOne hundred and forty-four anti-Toxoplasma IgM-positive pregnant women were prospectively tested for IgG avidity for Toxoplasma to determine the period of Toxoplasma infection. We adapted this serologic technique to a commercial ELISA kit "Toxo IgG EIA Seiken" (Denka Seiken, Japan) using 8 M urea as a dissociating agent.Results・The mean specific IgM of the pregnant women was 2.65 +/-1.36.・Of the 144 pregnant women 123 (85.4%) had high avidity results (indicative of previous infection), 9(6.3%) had low avidity results (indicative of recent infection), and 12 (8.3%) had borderline results.・All of 72 neonates had no physical and neurological abnormality. Three were LFD, 8 were HFD, and 55 were AFD.・neonates ; 72 cases without anomaly・positive anti-Toxoplasma IgM ; 0/66・anti-Toxoplasma IgM : 0.24±0.12 (m±SD)・abnormal pathological findings of placenta : 0/29・positive PCR in placenta and cord bloods : 0/16・Abnormal funduscopic findings : 0/36・Abnormal CT scan findings : 0/29・Half-life of anti-Toxoplasma IgG : 31.4±4.5 days (23 non-infected offspring)ConclusionsAs a high IgG avidity can differentiate previously inflected women from IgM-positive pregnant women, measurement of the avidity will avoid unnecessary amniocentesis, medication, and artificial abortion.

目的胎儿先天性弓形虫感染常导致新生儿出现衰弱性疾病。为了更好地评估母体感染的时间，我们检测了母体抗弓形虫IgG的亲和力。研究方法对144例抗弓形虫igm阳性孕妇进行弓形虫IgG抗体检测，确定弓形虫感染期。我们将这种血清学技术应用于商用ELISA试剂盒“弓形虫IgG EIA Seiken”（Denka Seiken，日本），使用8m尿素作为解离剂。·孕妇的平均特异性IgM为2.65±1.36。144例孕妇中，高贪婪结果123例（85.4%），低贪婪结果9例（6.3%），边缘性结果12例（8.3%）。72例新生儿均无生理和神经异常。LFD 3例，HFD 8例，AFD 55例。·新生儿；·抗弓形虫IgM阳性72例；·抗弓形虫IgM: 0.24±0.12 （m±SD）·胎盘病理异常：0/29·胎盘和脐带血PCR阳性：0/16·眼底镜异常：0/36·CT扫描异常：0/29·抗弓形虫IgG半衰期：结论IgG亲和度高可区分既往感染妇女和igm阳性孕妇，检测IgG亲和度可避免不必要的羊膜穿刺术、药物治疗和人工流产。

项目成果

検査値をどう読むか 産婦人科診療へのアプローチ.II.感染症.10. トキソプラズマ

如何解读测试值妇产科治疗方法二、弓形虫病。

• 发表时间: 2004
• 期刊: 産科と婦人科 71(増刊号)
• 作者: 小島俊行;武谷雄二
• 通讯作者: 武谷雄二

Mother-to-child Infection ; Virus 1.

母婴感染；

• 发表时间: 2004
• 期刊: ACTA OBSTETRICA ET GYNAECOLOGICA JAPONIKA 56(9)
• 作者: T.Kojima;Y.Taketani.;Takahashi T et al.;T.Kojima;篠崎博光;Takahshi T et al.;T.Kojima.
• 通讯作者: T.Kojima.

今日の治療と看護

今天的治疗和护理

• 发表时间: 2004
• 作者: B Pan;K Sengoku;et al.;Kurabayashi T. et al.;Yumiko Abe et al.;児島俊行(共著)
• 通讯作者: 児島俊行(共著)

小島俊行(共著): "産科臨床ベストプラクティス"医学書院. 335 (2004)

Toshiyuki Kojima（合著者）：“产科临床最佳实践”Igaku Shoin 335 (2004)。

周産期の治療薬マニュアル.10.原虫症1)トキソプラズマ

围产期治疗药物手册。 10.原虫 1）弓形虫。

• 发表时间: 2003
• 期刊: 周産期医学 33(増刊号)
• 作者: 小島俊行;堤 治;武谷雄二;Kasumi Shimizu;小島俊行
• 通讯作者: 小島俊行