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STUDIES ON FACTORS RELATED TO EMBRYO DEVELOPMENT TO IMPROVE OUTCOME OF ASSITED REPRODUCTIVE TECHNOLOGY

研究胚胎发育相关因素以提高辅助生殖技术的效果

基本信息

批准号：
12671580
负责人：
TSUTSUMI Osamu
金额：
$ 1.92万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671580/
关键词：
embryo low dose effect in vitro fertilization trophoblast outgrowth assay bisphenol A blastocyst estrogen receptor endocrine disrupter 内分泌攪乱科学物質

项目摘要

Bisphenol A (BPA), an estrogenic compound, is produced at more than 2 billion pounds/year and used in the production of epoxy resins and polycarbonate plastics. These plastics are used in many food and drink packaging applications, while the resins are commonly used as lacquers to coat metal products such as food cans, bottles tops and water supply pipes. It is also used in dentistry. Human exposure to BPA may be significant and raises the concern that these compounds may also be present in human reproductive organs and may affect reproductive processes including embryonic development, which are extremely sensitive to various of agents (1, causing declined fertility. We applied the preimplantation embryo development model to examine the effect of BPA which has ; been shown to exert effects similar to low-dose effects of endocrine disrupters. For the control group that was not exposed to BPA, the rate of in vitro development from two-cell embryos to eight-cell embryos was approximately 88% (334/378) at 24 hr of culture. This rate was significantly increased by exposure to 3 nM BPA(94% ; 172/182) compared with the rate for the control group. Similarly, the rates of development to the blastocyst stage in 48-hr cultures of two-cell embryos were significantly increased by exposure to BPA at concentrations of 1 nM (69.0% ; 207/300) and 3 nM (69.2% ; 126/182) compared with the unexposed control embryos (58.7% ; 222/378). On the other hand, the frequency of development to the blastocyst stage at 48 hr was significantly decreased by exposure to 100 μM BPA (31.2% ; 93/298) compared with that for the control group, although the development to eight-cell embryos at 24 hr was not inhibited by 100 μM BPA.

双酚A（BPA）是一种雌激素化合物，产量超过20亿磅/年，用于生产环氧树脂和聚碳酸酯塑料。这些塑料用于许多食品和饮料包装应用，而树脂通常用作涂料，用于涂覆金属产品，如食品罐，瓶盖和供水管。它也用于牙科。人类暴露于BPA可能是显著的，并引起人们的担忧，这些化合物也可能存在于人类生殖器官中，并可能影响生殖过程，包括胚胎发育，这对各种试剂非常敏感（1），导致生育能力下降。我们应用植入前胚胎发育模型来检查BPA的作用，BPA已被证明产生与低剂量内分泌干扰物类似的作用。对于未暴露于BPA的对照组，在培养24小时时，从2细胞胚胎到8细胞胚胎的体外发育率约为88%（334/378）。与对照组相比，暴露于3 nM BPA（94% ; 172/182）的该比率显著增加。同样，与未暴露的对照胚胎（58.7% ; 222/378）相比，暴露于1 nM（69.0% ; 207/300）和3 nM（69.2% ; 126/182）浓度的BPA后，48小时培养的双细胞胚胎发育至囊胚期的比率显著增加。另一方面，与对照组相比，暴露于100 μ M BPA时，48 h时发育至囊胚期的频率显著降低（31.2% ; 93/298），尽管100 μM BPA对24 h时发育至8细胞胚胎没有抑制作用。