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研究胚胎发育相关因素以提高辅助生殖技术的效果

基本信息

  • 批准号:
    12671580
  • 负责人:
  • 金额:
    $ 1.92万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2001
  • 项目状态:
    已结题

项目摘要

Bisphenol A (BPA), an estrogenic compound, is produced at more than 2 billion pounds/year and used in the production of epoxy resins and polycarbonate plastics. These plastics are used in many food and drink packaging applications, while the resins are commonly used as lacquers to coat metal products such as food cans, bottles tops and water supply pipes. It is also used in dentistry. Human exposure to BPA may be significant and raises the concern that these compounds may also be present in human reproductive organs and may affect reproductive processes including embryonic development, which are extremely sensitive to various of agents (1, causing declined fertility. We applied the preimplantation embryo development model to examine the effect of BPA which has ; been shown to exert effects similar to low-dose effects of endocrine disrupters. For the control group that was not exposed to BPA, the rate of in vitro development from two-cell embryos to eight-cell embryos was approximately 88% (334/378) at 24 hr of culture. This rate was significantly increased by exposure to 3 nM BPA(94% ; 172/182) compared with the rate for the control group. Similarly, the rates of development to the blastocyst stage in 48-hr cultures of two-cell embryos were significantly increased by exposure to BPA at concentrations of 1 nM (69.0% ; 207/300) and 3 nM (69.2% ; 126/182) compared with the unexposed control embryos (58.7% ; 222/378). On the other hand, the frequency of development to the blastocyst stage at 48 hr was significantly decreased by exposure to 100 μM BPA (31.2% ; 93/298) compared with that for the control group, although the development to eight-cell embryos at 24 hr was not inhibited by 100 μM BPA.
双酚A(BPA)是一种雌激素化合物,产量超过20亿磅/年,用于生产环氧树脂和聚碳酸酯塑料。这些塑料用于许多食品和饮料包装应用,而树脂通常用作涂料,用于涂覆金属产品,如食品罐,瓶盖和供水管。它也用于牙科。人类暴露于BPA可能是显著的,并引起人们的担忧,这些化合物也可能存在于人类生殖器官中,并可能影响生殖过程,包括胚胎发育,这对各种试剂非常敏感(1),导致生育能力下降。我们应用植入前胚胎发育模型来检查BPA的作用,BPA已被证明产生与低剂量内分泌干扰物类似的作用。对于未暴露于BPA的对照组,在培养24小时时,从2细胞胚胎到8细胞胚胎的体外发育率约为88%(334/378)。与对照组相比,暴露于3 nM BPA(94% ; 172/182)的该比率显著增加。同样,与未暴露的对照胚胎(58.7% ; 222/378)相比,暴露于1 nM(69.0% ; 207/300)和3 nM(69.2% ; 126/182)浓度的BPA后,48小时培养的双细胞胚胎发育至囊胚期的比率显著增加。另一方面,与对照组相比,暴露于100 μ M BPA时,48 h时发育至囊胚期的频率显著降低(31.2% ; 93/298),尽管100 μM BPA对24 h时发育至8细胞胚胎没有抑制作用。

项目成果

期刊论文数量(29)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Matsumi H: "Regulation of Nitric Oxide Synthase to Promote Cytostsis in Ovarian Follicular Development."Biol Reprod.. 63(1). 141-146 (2000)
Matsumi H:“调节一氧化氮合酶促进卵巢卵泡发育中的细胞停滞”。Biol Reprod.. 63(1)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Tsutsumi O: "Estrogen receptor-mediated effects of a xenoestrogen, bisphenol a, on preimplantation mouse embryos"Biochem Biophys Res Commun. 270(3). 918-921 (2000)
Tsutsumi O:“雌激素受体介导的异种雌激素双酚 A 对植入前小鼠胚胎的影响”Biochem Biophys Res Commun。
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  • 影响因子:
    0
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  • 通讯作者:
Takai Y: "Preimplantation exposure to bisphenol A advances postnatal development"Reproductive Toxicology. 15(1). 71-74 (2001)
Takai Y:“植入前接触双酚 A 可促进产后发育”生殖毒理学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Takai Y, Tsutsumi O, Ikezuki Y, Hiroi H, Osuga Y, Momoeda M, Yano T, Taketani Y: "Estrogen receptor-mediated effects of a xenoestrogen, bisphenol A, on prei mplantation mouse embryos"Biochem Biophys Res Commun.. 270(3). 918-921 (2000)
Takai Y、Ttsutsumi O、Ikezuki Y、Hiroi H、Osuga Y、Momoeda M、Yano T、Taketani Y:“雌激素受体介导的异雌激素双酚 A 对植入前小鼠胚胎的影响”Biochem Biophys Res Commun.. 270
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Osuga Ji: "Targeted disruption of hormone-sensitive lipase results in male sterility and adipocyte hypertrophy, but not in obesity"Proc Natl Acad Sci USA. 97(2). 787-792 (2000)
Osuga Ji:“激素敏感性脂肪酶的靶向破坏会导致男性不育和脂肪细胞肥大,但不会导致肥胖”Proc Natl Acad Sci USA。
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    0
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TSUTSUMI Osamu其他文献

TSUTSUMI Osamu的其他文献

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{{ truncateString('TSUTSUMI Osamu', 18)}}的其他基金

White-color luminescence from a single material with harmony between order- and disorder-structures of luminogen aggregates
单一材料的白色发光,发光体聚集体的有序结构和无序结构之间的协调
  • 批准号:
    15K05613
  • 财政年份:
    2015
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Self-organization of gold nanoparticles and photochemical manipulation of their organized structures by means of liquid-crystalline molecules
金纳米粒子的自组织及其通过液晶分子对其组织结构进行光化学操纵
  • 批准号:
    21710094
  • 财政年份:
    2009
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Development of Photoresponsive Organic-Inorganic Hybrid Liquid Crystals : Spontaneous Organization of Inorganic Nanoparticles with Organic Molecules
光响应有机-无机杂化液晶的开发:无机纳米粒子与有机分子的自发组织
  • 批准号:
    19559003
  • 财政年份:
    2007
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A laboratory and clinical study of the mechanism of mother-to-child infection of Toxoplasma
弓形虫母婴感染机制的实验室及临床研究
  • 批准号:
    15591732
  • 财政年份:
    2003
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
BASIC STUDIES FOR IMPROVEMENT OF IN VITRO FERTILIZATION AND CRYOPRESERVATION
改进体外受精和冷冻保存的基础研究
  • 批准号:
    10671518
  • 财政年份:
    1998
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analysis of the microenvironment and cryopreservation of embryos on their development
胚胎发育的微环境和冷冻保存分析
  • 批准号:
    08671870
  • 财政年份:
    1996
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Assessmemt of embryo viability and its microenvironment
胚胎活力及其微环境的评估
  • 批准号:
    06671632
  • 财政年份:
    1994
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Biochemical and endocrinological analysis of oocytes and embryos
卵母细胞和胚胎的生化和内分泌分析
  • 批准号:
    03670771
  • 财政年份:
    1991
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Physiological roles of growth factors in mammalian reproduction.
生长因子在哺乳动物繁殖中的生理作用。
  • 批准号:
    63570771
  • 财政年份:
    1988
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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The study of late low dose effect to thyroid disease in Marshall Islands
马绍尔群岛甲状腺疾病后期低剂量效应研究
  • 批准号:
    07671285
  • 财政年份:
    1995
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