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Developmental Research for Confocal Laser Microscope to Realize Fluorescence Microtomography.

共焦激光显微镜实现荧光显微断层扫描的发展研究。

基本信息

批准号：
63870019
负责人：
FUJITA Setsuya
金额：
$ 12.54万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B).
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

True information in histopathology resides in tree-dimensional space, either in cellular level or in histological structures. Until present, however, most information on microscopic morphology is limited within two-dimensional space. Cancer diagnosis, has been, as well known, based on structural atypism of glandular contour, organization of cell nuclei, or chromatin distribution, etc. All of them are three-dimensional. So far stereoscopic viewing by tilting the object has been adopted to satisfy quasi-3D feeling. This method, however, is nothing to do with quantitative information of fluorescent materials in the 3D space. This stereoscopy is really superficial.To overcome these problems, flurorescence microtomography by using a confocal microscope is the most suitable. The present investigation aims at building a confocal microscope system to realize semi-automatically the fluorescence micro tromography in quantitative terms.We developed with colloboration with Research Group of Olympu … More s Optic Co. A confocal fluorescent microscope system which can obtain serial optic sections of semitransparent object, semi-automatically. The series of images are stored in laser disc and can be retrieved at any time to construct stereograms viewed at any angle. Each tomograph, however, represents quantitative aspect of fluorescent materials in that plane, so that summation of the pixel values produce the value of total material present in that plane or in that structure. This system is proved useful for application of quantitation of aimed materials distributed in 3D space in microscopic object.Results of the research, summarized above, indicates that the confocal system constructed in the present developmental research is useful and powerful in future investigation of 3D object. Nevertheless, further improvement would be necessary to perfect and accomplish quantitative 3D microscopy, since the time spent for investigation on this system is still short in comparison with usual light microscope which had been developed over 100 and more years. Less

组织病理学中的真实信息存在于三维空间中，无论是在细胞水平还是在组织结构中。然而，到目前为止，大多数关于微观形态的信息都局限于二维空间。众所周知，癌症的诊断是基于腺体轮廓的结构相似性、细胞核的组织结构或染色质分布等，它们都是三维的。到目前为止，通过倾斜物体的立体观看已经被采用以满足准3D感觉。然而，该方法与荧光材料在3D空间中的定量信息无关。为了克服这些问题，使用共聚焦显微镜的荧光显微层析成像是最合适的。本研究的目的是建立一个半自动荧光显微成像系统，实现荧光显微成像的定量分析。 ...更多信息共焦荧光显微镜系统，可以半自动地获得连续的光学切片。这一系列图像存储在激光光盘中，可以随时检索，以构建从任何角度观察的立体图。然而，每个断层图像表示该平面中荧光材料的定量方面，使得像素值的总和产生存在于该平面或该结构中的总材料的值。该系统被证明对于微观物体中分布在3D空间中的目标材料的定量应用是有用的。以上总结的研究结果表明，目前发展研究中构建的共焦系统在未来的3D物体研究中是有用且强大的。然而，进一步的改进将是必要的，以完善和实现定量3D显微镜，因为花费在这个系统上的调查时间仍然很短，与普通的光学显微镜相比，已经开发了100多年。少