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Permanent Effect of Hormones and Their Related Compounds on Target Organs

激素及其相关化合物对靶器官的永久性影响

基本信息

批准号：
01540618
负责人：
IGUCHI Taisen
金额：
$ 1.28万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01540618/
关键词：
Diethylstilbestrol Irreversible change Polyovular follicles Tamoxifen Critical period Sexual dimorphism Pelvis Blood glucose Tamoxifeu

项目摘要

Permanent effects of hormones and their related compounds on gonadーaccessory and neoplastic tissues were studied.1. Tamoxifen. (1) Tamoxifen given neonatally induced degeneration of uterine glands, myometrium and oocytes in females, and (2) suppression of fibrocartilage formation in penis and suppression of spermatogenesis in males. (3) Critical periods of induction by tamoxifen of abnormalities in female and male mice were 7 and 3 days after birth, respectively. (4) Tamoxifen directly suppressed the ossification of pelvis in vitro.2. Sexual dimorphism of pelvis. (1) Sexual dimorphism in pelvis was formed by 30 days of age. Pubis in females is longer and thinner than the males. Ischium in males is wider and larger than the females. (2) Studies on pelvis of Tfm mouse and sequential gonadectomy revealed that shapes of pubis and ischium were controlled by estrogen and androgen, respectively.3. Polyovular follicles. (1) Polyovular follicles induced by neonatal treatment with diethylstilbestol (DES) can be ovulated by gonadotropins and (2) ova from polyovular follicles can be fertilized in vitro, though the fertilization rate is lower than those of ova from uniovular follicles from controls and neonatally DES-exposed mice.4. Tumor and blood glucose. (1) Mice developed mammary tumor showed higher blood glucose and insulin than mice without the tumor. There is a positive correlation between mammary tumor size and blood glucose. In these mice, liver was larger than the controls. (2) Mice transplanted Lewis lung carcinoma showed higher blood glucose.5. Protein profiles in irreversibly changed vagina. Two dimensional electrophoresis reveled that similar proteins were expressed both in vagina of ovariectomized mice treated neonatally with DES and in ovariectomized adult mice given injections of DES just before sacrifice. These results indicate that some proteins were permanently expressed in DES-exposed vagina.

研究激素及其相关化合物对性腺、ー附件和肿瘤组织的永久性影响。他莫昔芬。(1)新生儿服用他莫昔芬可导致女性子宫腺、子宫肌层和卵母细胞变性，(2)抑制男性阴茎纤维软骨形成和精子生成。(3)三苯氧胺诱发雌性和雄性小鼠畸形的临界期分别为出生后7d和3d。(4)三苯氧胺可直接抑制体外骨盆骨化。骨盆的性二形畸形。(1)30日龄时骨盆形成性别二型性；雌性的耻骨比雄性的长而薄。雄性坐骨比雌性坐骨更宽、更大。(2)对TFM小鼠骨盆和序贯切除性腺的研究表明，耻骨和坐骨的形状分别受雌激素和雄激素的控制。多卵泡卵泡。(1)己烯雌酚(DES)诱导的多卵泡卵泡能被促性腺激素排卵；(2)多卵泡卵泡的受精率低于对照组和去甲基己烯雌酚(DES)暴露组小鼠的单卵泡卵母细胞受精率。肿瘤和血糖。(1)发生乳腺肿瘤的小鼠血糖和胰岛素水平高于未发生肿瘤的小鼠。乳腺肿瘤大小与血糖呈正相关。在这些小鼠中，肝脏比对照组大。(2)小鼠移植Lewis肺癌后血糖升高。不可逆变化的阴道中的蛋白质图谱。双向凝胶电泳法显示，新生的去卵巢小鼠和成年去卵巢小鼠在处死前注射DES的阴道中都有相似的蛋白质表达。这些结果表明，一些蛋白质在DES暴露的阴道中永久表达。