Development of reverse-genetics methods for Daphnids

水蚤反向遗传学方法的开发

• 批准号: 22651078
• 负责人: IGUCHI Taisen
• 金额: $ 2.16万
• 依托单位: National Institutes of Natural Sciences Okazaki Research Facilities
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22651078/
• 关键词: ミジンコ; 逆遺伝学; エコゲノミクス

The water flea Daphnia magna is a small crustacean which is an ubiquitous inhabitant in fresh water. As daphnids inhabit a diverse array of aquatic environments, they are a good model for understanding response and adaptation to environmental changes and evolution. Therefore, they have been used one of standard organisms in ecotoxicology. While daphnids have significant importance in ecotoxicology, tools to understand response to environmental changes in daphnia is very limited. We investigate a condition for natural male production and improve introduction of foreign DNA into daphnids. These basic information will be available for establishment of transgenic daphnids.

水蚤是一种小型甲壳类动物，在淡水中无处不在。由于水蚤栖息在各种各样的水生环境中，它们是理解对环境变化和进化的反应和适应的一个很好的模型。因此，它们已被用作生态毒理学的标准生物之一。虽然水蚤在生态毒理学中具有重要意义，但了解水蚤对环境变化反应的工具非常有限。我们研究了自然雄性产生的条件，并改进了将外源DNA导入水蚤的方法。这些基本信息将为转基因蚤类的建立提供依据。

项目成果

Endocrine disrupters issues in Japan

日本的内分泌干扰物问题

• 发表时间: 2011
• 作者: Iguchi;T.;er al
• 通讯作者: er al

Demasculinization and feminization of male gonads by atrazine: consistent effects across vertebrate classes.

• DOI: 10.1016/j.jsbmb.2011.03.015
• 发表时间: 2011-10
• 期刊: JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
• 影响因子: 4.1
• 作者: Hayes, Tyrone B.;Anderson, Lloyd L.;Beasley, Val R.;de Solla, Shane R.;Iguchi, Taisen;Ingraham, Holly;Kestemont, Patrick;Kniewald, Jasna;Kniewald, Zlatko;Langlois, Valerie S.;Luque, Enrique H.;McCoy, Krista A.;Munoz-de-Toro, Monica;Oka, Tomohiro;Oliveira, Cleida A.;Orton, Frances;Ruby, Sylvia;Suzawa, Miyuki;Tavera-Mendoza, Luz E.;Trudeau, Vance L.;Victor-Costa, Anna Bolivar;Willingham, Emily
• 通讯作者: Willingham, Emily

Sex determination mechanisms of water flea, Daphnia magna

水蚤、大型溞的性别决定机制

• 发表时间: 2010
• 作者: Toyota K.;Iguchi T.;et al.;Iguchi T.
• 通讯作者: Iguchi T.

ミジンコDaphnia pulexにおける捕食者カイロモンに応じた胚発生過程の変化

水溞中胚胎发育对捕食者利它素的反应的变化

• 发表时间: 2011
• 作者: 石川麻乃、宮川一志;他6名;宮川一志;宮川一志
• 通讯作者: 宮川一志

Endocrine disruption in invertebrates : Sex determination of Daphnia magna

无脊椎动物的内分泌干扰：大型溞的性别决定

• 发表时间: 2011
• 作者: Iguchi;T
• 通讯作者: T