Development of reverse-genetics methods for Daphnids

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The water flea Daphnia magna is a small crustacean which is an ubiquitous inhabitant in fresh water. As daphnids inhabit a diverse array of aquatic environments, they are a good model for understanding response and adaptation to environmental changes and evolution. Therefore, they have been used one of standard organisms in ecotoxicology. While daphnids have significant importance in ecotoxicology, tools to understand response to environmental changes in daphnia is very limited. We investigate a condition for natural male production and improve introduction of foreign DNA into daphnids. These basic information will be available for establishment of transgenic daphnids.