Linkage analysis between gene for grain shattering and semi-dwarfing gene and character expression of shattering in rice

水稻落粒基因与半矮化基因连锁分析及落粒性状表达

• 批准号: 01560002
• 负责人: KIKUCHI Fumio
• 金额: $ 1.41万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01560002/
• 关键词: Oryza sativa; shattering gene; semi-dwarfing gene; linkage; abscission layer; 脱粒性遺伝子; 半矮性遺伝子; 離層組織; Oryza sativa L.

The development of semi-dwarf rice cultivars has brought about a breakthrough in yield potential of yield in many rice growing countries of the world. It has become apparent that most semi-dwarf rice cultivars in the tropical and temperate regions carry the semi-dwarfing gene at the same locus As the Dee-geo-woo-gen gene sd-1.The linkage analysis between the semi-dwarfism and the shattering habit in both indica semidwarf cultivar Taicuiig Native 1 and japonica semi-dwarf cultivar Shiranui were carried out. The japonica tall cultivar Norin 29 and the two near-isogenic lines SC2 and SC4 were used in this study. SC2 and SC4 have both semi-dwarfing gene sd-1 and shattering gene derived from Taichung Native 1 and Shiranui, respectively. The degree of shattering was measured by an, apparatus for measuring the breaking tensile strength of the base of sterile lemma. The segregation patterns of shattering habit in the F_2s of SC2/Norin 29 and SC4/Norin 29 showed fit to a 3 non-shattering : 1 shattering genetic ratio, indicating that both SC2 and SC4 carry a single recessive gene controlling shattering habit, respectively. The F_1 and F_2 of SC2/SC4 showed the same shattering habit as that of both parents. Therefore, both SC2 and SC4 were considered to have the same shattering gene. The linkage relationship between the semi-dwarfing gene and the shattering gene was observed in the F_2s of SC2/Norin 29 and SC4/Norin 29. The weighted average of the recombination values estimated from the two-crosses was 13.1 %. A Chinese semi-dwarf cultivar was also proved to have the sd-l and the shattering gene linked to the sd-l in the 1st chromosome. From these result, it is assumed that the shattering gene has been transmitted to many semi-dwarf cultivars together with the sd-l in the breeding programs of semi-dwarf high-yielding cultivars. This shattering gene was symbolized sh-2.The abscission layer at the base of sterile lemma was observed before heading time.

半矮秆水稻品种的开发使世界许多水稻种植国的产量潜力实现了突破。现已发现，热带和温带地区的大多数半矮化水稻品种都携带与Dee-geo-woo-gen基因sd-1位于同一位点的半矮化基因。 出去。本研究使用粳稻高品种 Norin 29 和两个近等基因系 SC2 和 SC4。 SC2和SC4分别具有源自台中本地1号和不知火的半矮化基因sd-1和破碎基因。通过用于测量无菌外稃基底的断裂拉伸强度的装置来测量破碎程度。 SC2/Norin 29 和 SC4/Norin 29 的 F_2 中破碎习性的分离模式显示出符合 3 不破碎: 1 破碎遗传比，表明 SC2 和 SC4 分别携带控制破碎习性的单个隐性基因。 SC2/SC4的F_1和F_2表现出与父母双方相同的破碎习惯。因此，SC2和SC4被认为具有相同的破碎基因。 SC2/Norin 29和SC4/Norin 29的F_2中观察到半矮化基因和破碎基因之间的连锁关系。由两次杂交估计的重组值的加权平均值为13.1%。一个中国半矮化品种也被证明具有sd-1和与第1染色体上的sd-1连锁的破碎基因。从这些结果推测，在半矮化高产品种的育种计划中，破碎基因已与sd-l一起被传递到许多半矮化品种中。该破碎基因记为sh-2。在抽穗前观察不育外稃基部的离层。

项目成果

Oba.S and F.Kikuchi: "Formation of abscission layer controlled by a shattering gene linked with a semidwarfing gene sd-1 in rice" Rice Genetics Newsletters. 6. 132-134 (1989)

Oba.S 和 F.Kikuchi：“由与水稻半矮化基因 sd-1 相关的破碎基因控制的脱落层的形成”水稻遗传学通讯。

Oba, S. and F. Kikuchi: "Formation of abscission layer controlled by a shattering gene linked with a semi-dwarfing gene sd-1 in rice." Rice Genetics Newsletters. 6. 132-134 (1989)

Oba, S. 和 F. Kikuchi：“由与水稻半矮化基因 sd-1 相关的破碎基因控制的脱落层的形成。”

Oba, S. and F. Kikuchi: "Identification of semi-dwarf mutant gene carried by rice semi-dwarf line X 46." Bull. Agr. & For. Res. U. Tsukuba. 3. 1-7 (1991)

Oba, S. 和 F. Kikuchi：“水稻半矮秆系 X 46 携带的半矮秆突变基因的鉴定”。

大場伸哉: "イネの半矮性遺伝子sd-1と脱粒性遺伝子の連鎖 第1報 インド型半矮性品種「台中在来1号」と日本型半矮性品種「シラヌイ」における半矮性遺伝子の連鎖分析" 熱帯農業. 33. 286-291 (1989)

Shinya Oba：“水稻半矮化基因sd-1与脱落基因的连锁。首次报告：印度型半矮化品种‘台中本土1号’与日本半矮化基因的连锁分析型半矮化品种“不知火””热带农业。33。286-291（1989）

大場 伸哉,鶴貝 裕子,菊池 文雄: "イネの半矮性遺伝子sdー1と脱粒性遺伝子の連鎖。第1報インド型半矮性品種台中在来1号と日本型半矮性品種シラヌイにおける半矮性・遺伝子sdー1と脱粒性・遺伝子の連鎖分析" 熱帶農業. 33. 286-291 (1989)

Shinya Ohba、Yuko Tsurugai、Fumio Kikuchi：“水稻半矮化基因 sd-1 与脱落基因之间的联系。首次报道：印度型半矮化品种台中本土 1 号和日本型半矮化品种型半矮化品种 Shiranui。“基因 sd-1 和脱落特性/基因的连锁分析”Neksha Nogyo。 33. 286-291 (1989)