Utilization of Fungicide-Resistant Strain of Rhizobium on the Cultivation of Leguminous Crops

本研究旨在通过种子接种抗杀菌剂的根瘤菌菌株来提高豆科作物的生长和固氮。主要研究结果如下：1. TMTD（福美双）完全抑制了B的生长。对大豆根瘤菌的生长影响不大。菜豆2.春雷霉素种子处理对土壤培养大豆植株的生长没有明显的抑制作用。相反，处理显著抑制了由原生根瘤菌诱导的根瘤形成.春雷霉素和春雷霉素耐药菌株B的同时治疗。大豆根瘤菌促进大豆植株的生长和固氮作用.活性炭拌种对春雷霉素和抗春雷霉素B菌株接种的大豆植株的生长和固氮有明显的促进作用。japonica.此外，过氧化钙包衣苜蓿种子，促进了种子的萌发和早期生长.苜蓿叶蛋白生产过程中产生的植物汁液可用作根瘤菌的培养基。菜豆固氮活性低是根瘤形成小而固氮活性差的主要原因。

The purpose of this investigation is to improve the plant growth and nitrogen fixation of leguminous crops by seed inoculation of a fungicide-resistant strain of rhizobium. The results obtained were as follows:1. TMTD (Thiram) completely inhibited the growth of B. japonicum, while it had little effect on the growth of R, leguminosarum bv. phaseoli.2. Growth of soybean plants cultured in soil was not appreciably suppressed by seed treatment of kasugamycin. On the contrary, the treatment markedly suppressed nodule formation induced by native rhizobia.3. Simultaneous treatment of kasugamycin and kasugamycin-resistant strain of B. japonicum improved the growth and nitrogen fixation of soybean plants.4. Seed coating by charcoal was effective for the growth and nitrogn fixation of soybean plants inoculated with kasugamycin and kasugamycin-resistant strain of B. japonecu. Besides, the coating of alfalfa seed by calcium peroxide promoted the germination and early growth of plants.5. Alfalfa plant juice produced from leaf protein production process was useful as a culture medium for rhizobium.6. Low nitrogen fixation of kidney bean was found to be responsible for formation of small size nodules with poor nitrogen fixing activity.