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Applied Studies on Lytic Enzyme-Producing Microorganisms

产裂解酶微生物的应用研究

基本信息

批准号：
01560223
负责人：
SUGAHARA Isao
金额：
$ 1.41万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

Microorganisms capable of lysing the bacterial cells at 5^ﾟC Were infrequent in marine environments, compared to those capable of lysing the cells at 20-25^ﾟC. Out of 33 strains of lytic enzyme-producing bacteria isolated, Bacillus (81.8%) Was the most dominant bacterial genus. seudomonas and Vibrio comprised 18.2% of the bacteriolytic bacteria isolated. The optimum temperature for production of lytic enzyme of the representative strains Bacillus sp. T301,T303 and E33 was around 13-17^ﾟC. The optimum temperature for lytic activity of strains T301, T303 and E33 was around 30-40^ﾟC.Myco-lytic microorganism strain No.5 was isolated from mangrove swamp. Strain No.5 was found to produce myco-lytic enzyme when aerobically grown at 30^ﾟC in 90% seawater medium containing polypepton (0.5%) and vyast extract (0.3%). The optimum PH and temperature for lytic activity of strain No. 5 were around 9 and 50-60^ﾟC, respectively. Lytic activity was completely inactivated at 70^ﾟC and 10 min. Lytic activity was inhibited by 0.05-0.20 M of inorganic salts such as NaC1, KC1, Mgcl_2, Cacl_2,_2, and 0.1 mM of protease inhibitors such as leupeptin, apain, phosphoramidon and E 64.An attempt to increase in myco-lysis using chitin-decomposing bacteria isolated was made. Lytic activity was enhanced about 1.5 times by the combination of strain No.5 enzyme and chitinolytic strain F190 enzyme.

在海洋环境中，能够在5ﾟC下裂解细菌细胞的微生物并不多见，而在20~25ﾟC下能够裂解细菌细胞的微生物却很少。假单胞菌和弧菌占分离菌株的18.2%。确定了代表菌株芽孢杆菌裂解酶产生的最适温度。菌株T301、T303和E33的最适裂解温度为30~40ﾟC，菌株T301、T303和E33的最适裂解温度为13~17ﾟC。5号菌株在含有多肽(0.5%)和Vyast提取物(0.3%)的90%海水培养基中，在30ﾟC好氧条件下能产生溶菌酶。菌株5的最适pH为9ﾟC左右，最适温度为50~60℃。裂解活性在70ﾟC和10min时完全失活。0.05-0.20M的无机盐NaC_1、KC_1、MgCl_2、CaCl_2、_2和0.1 mM的蛋白水解酶抑制剂，如亮氨酸酶、蛋白水解酶、磷酰胺和E 64，均能抑制其溶菌活性。菌株5号和降解几丁质的菌株F190联合使用，其裂解活力提高了1.5倍。