Studies on microbiological remediation in closed bay

封闭海湾微生物修复研究

• 批准号: 07660242
• 负责人: SUGAHARA Isao
• 金额: $ 1.41万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660242/
• 关键词: bioremediation; microbial function; environment inprovement; dnitrification; closed bay; 微生物開発・利用; バイオリメデイエーション; バイオリメティエーション

1.In order to improve coastal environment (closed bay etc.), it is necessary to decrease in organic matter level and inorganic nutrients (nitrogen or phosphorus) level. In this study, we set up microbial system containing denitrifying bacteria, ammonia-oxidizing bacteria and micro-algae. All microorganisms were tried to isolate from polleted bottom mud.2.The optimum temperature and pH for denitrification activity of immobilized denitrifying bacteria strain Sc51 were 30ﾟC and pH7-8, respectively. The maximum denitrifying activity (Vmax) of strain C8-2 was 3.74 mumol/hr, and NO_3^- affinity of denitrifying activity was 40.0mumol/l.The discharged water from waste treatment facility was treated with denitrifying bacteria strain Sc51. Strain Sc51 effectively removed nitrate and/or nitrite. The eliminating activity was 55 mumol/hr.Useful micro-algae strains capable of strongly producing oxygen under low light condition, and weakly cosuming oxygen under dark condition were selected. The addit … More ion of microalgae strain P-1 to mixture of in situ seawater and bottom mud was able to improve oxygen poor environment.An attempt to utilize useful marine microorganisms was carried out for the improving polleted environment. Ceramic grains with high porosity (porous beads) were used as microbial carrier. Denitrifying bacteria were attached on and into the beads. Ammonia-oxidizing bacteria mixpopulation and micro-algae were also attached on the surface of the beads. The beads prepared were spread on the bottom mud of polluted closed bay on January and July. The results obtained were as follows :a)The beads recovered from the bottom mud contained denitrifying bacteria and ammonia-oxidizing bacteria.b)When several types of bacterial numbers in bottom mud were compared, no significant difference could be obsevedc)The organic matter content and total nitrogen content of bottom mud markedly decreased after the beads spread.d)The in situ eliminating activities of organic matter and nirtrogen were high in summer. However, about 1/2-1/3 of the summer activities were observed in winter.6.Environment improvement or control by useful microbial functions will be expected to practical use in the fields of fisheries and environmental science. Less

1.为了改善沿海环境（封闭海湾等），有必要降低有机质水平和无机营养物质（氮或磷）水平。本研究建立了含反硝化菌、氨氧化菌和微藻的微生物系统。所有微生物均从污染的底泥中分离得到。固定化反硝化菌Sc51的最佳反硝化温度为30℃，pH为pH7-8。菌株C8-2的最大反硝化活性（Vmax）为3.74 mumol/hr， NO_3^-亲和力为40.0mumol/l。用Sc51反硝化菌对污水处理装置出水进行处理。菌株Sc51能有效去除硝酸盐和/或亚硝酸盐。清除活性为55 μ mol/hr。选择弱光条件下产氧强、暗光条件下耗氧弱的有用微藻菌株。微藻菌株P-1在原位海水和底泥混合物中添加更多离子能够改善缺氧环境。为改善污染环境，进行了利用有益海洋微生物的尝试。采用高孔隙率的陶瓷颗粒（多孔微珠）作为微生物载体。反硝化细菌附着在小球上并进入小球内。氨氧化混合菌群和微藻也附着在微球表面。于1月和7月将制备的微球铺在污染封闭海湾的底泥上。结果表明：a)从底泥中回收的微球中含有反硝化细菌和氨氧化细菌。b)对底泥中几种细菌的数量进行比较，发现差异不显著。c)微球扩散后，底泥有机质含量和全氮含量显著降低。d)夏季对有机质和氮的原位去除活性较高。然而，大约1/2-1/3的夏季活动发生在冬季。通过有益的微生物功能来改善或控制环境有望在渔业和环境科学领域得到实际应用。少

项目成果

Sugahara, Isao: "Bioremediation method for closed bays." Res.J.Food and Agri.19. 20-24 (1996)

菅原功：“封闭海湾的生物修复方法。”

Sugahara, Isao: "Sediment bacteria in inland waters." Nippon Suisan Gakkaishi. 64(2). 307-308 (1998)

菅原功：“内陆水域的沉积物细菌。”

岩田友三・菅原 庸・木村俊夫・佐野元宏・水口忠久・西村昭史 他: "五ヶ所湾におけるGymnodinium mikimoyoiの増殖ポテンシャル" 日本水産学会誌. 63(4). 578-584 (1997)

Yuzo Iwata、Tsune Sukawara、Toshio Kimura、Motohiro Sano、Tadahisa Mizuguchi、Akira Nishimura 等：“Gymnodinium mikimoyoi 在 Gokasho 湾的增殖潜力”日本水产学会杂志 63（4）（1997 年）。 ）

松岡行利・山川雅弘・柏 雅美・菅原 庸: "微生物の機能を利用した水質浄化(第1報)-低窒素同化細菌の生育特性-" 三重県環境科学センター報告. No.17. (1997)

松冈幸利、山川雅宏、柏雅美、菅原恒：“利用微生物的功能净化水质（第1部分）-低氮同化细菌的生长特性-”三重县环境科学中心报告书第17号（1997年）。

菅原 庸: "生物機能を使った内湾環境修復技術" 農林水産技術研究ジャーナル. 19(12). 20-24 (1996)

菅原恒：“利用生物功能的内湾环境恢复技术”农林水产技术研究杂志19（12）（1996）。