Studies on microbiological remediation in closed bay

1.为了改善近海环境(封闭海湾等)，必须降低有机质水平和无机营养盐(氮或磷)水平。本研究建立了由反硝化细菌、氨氧化细菌和微藻组成的微生物系统。固定化反硝化菌SC51的最适温度为30ﾟC，最适pH为7~8。菌株C8-2的最大反硝化活性(Vmax)为3.74mumol/hr，反硝化活性的NO3~-亲和力为40.0mumol/l。菌株Sc51能有效去除硝酸盐和/或亚硝酸盐。筛选出在弱光条件下产氧能力强、黑暗条件下产氧能力弱的优良微藻菌株。The Add…微藻菌株P-1向就地海水和底泥混合液中添加更多的离子可以改善低氧环境，为利用有用的海洋微生物改善养殖环境进行了尝试。采用孔隙率较高的陶瓷颗粒(多孔珠)作为微生物载体。反硝化细菌附着在珠子上和珠子里。微球表面还附着有氨氧化细菌、混合菌群和微藻。1月和7月，将制备的珠子涂抹在受污染的封闭海湾的底泥上。结果表明：(1)底泥中回收的颗粒含有反硝化细菌和氨氧化细菌；(2)底泥中几种细菌的数量比较，差异不显著；(3)铺设颗粒后底泥中有机质含量和总氮含量显著降低；(4)夏季底泥中有机物和氮素的就地去除活性较高。然而，大约1/2-1/3的夏季活动是在冬季观察到的。6.通过有用的微生物功能改善环境或控制环境将有望在渔业和环境科学领域得到实际应用。较少

1.In order to improve coastal environment (closed bay etc.), it is necessary to decrease in organic matter level and inorganic nutrients (nitrogen or phosphorus) level. In this study, we set up microbial system containing denitrifying bacteria, ammonia-oxidizing bacteria and micro-algae. All microorganisms were tried to isolate from polleted bottom mud.2.The optimum temperature and pH for denitrification activity of immobilized denitrifying bacteria strain Sc51 were 30ﾟC and pH7-8, respectively. The maximum denitrifying activity (Vmax) of strain C8-2 was 3.74 mumol/hr, and NO_3^- affinity of denitrifying activity was 40.0mumol/l.The discharged water from waste treatment facility was treated with denitrifying bacteria strain Sc51. Strain Sc51 effectively removed nitrate and/or nitrite. The eliminating activity was 55 mumol/hr.Useful micro-algae strains capable of strongly producing oxygen under low light condition, and weakly cosuming oxygen under dark condition were selected. The addit … More ion of microalgae strain P-1 to mixture of in situ seawater and bottom mud was able to improve oxygen poor environment.An attempt to utilize useful marine microorganisms was carried out for the improving polleted environment. Ceramic grains with high porosity (porous beads) were used as microbial carrier. Denitrifying bacteria were attached on and into the beads. Ammonia-oxidizing bacteria mixpopulation and micro-algae were also attached on the surface of the beads. The beads prepared were spread on the bottom mud of polluted closed bay on January and July. The results obtained were as follows :a)The beads recovered from the bottom mud contained denitrifying bacteria and ammonia-oxidizing bacteria.b)When several types of bacterial numbers in bottom mud were compared, no significant difference could be obsevedc)The organic matter content and total nitrogen content of bottom mud markedly decreased after the beads spread.d)The in situ eliminating activities of organic matter and nirtrogen were high in summer. However, about 1/2-1/3 of the summer activities were observed in winter.6.Environment improvement or control by useful microbial functions will be expected to practical use in the fields of fisheries and environmental science. Less