Ultrastructural Analysis of Functional Properties of the Glomerular Filtration Barrier

肾小球滤过屏障功能特性的超微结构分析

• 批准号: 01570003
• 负责人: SAKAI Tatsuo
• 金额: $ 1.34万
• 依托单位: Juntendo University School of Medicine (1990)The University of Tokyo (1989)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570003/
• 关键词: Glomerulus; Basement membrane; Bowman's space; Proximal tubule; Ultrastructure; 腎臓

(1) Ultrastructure of the glomerulus : The rat kidneys were processed for TEM with a cold dehydration technique to visualize the ultrastructure of the glomerulus including the mesangium. The mesangial cells possessed many processes containing abundant actin filaments. The processes make frequent contact with the glomerular basement membrane (GBM) obviously to anchor it. The anchoring is thought to develop wall tention to counterbalance the capillary blood pressure.(2) Ultrastructure of GBM : The ultrastructure of GBM is visualized either with TEM after cold-dehydration technique or with high resolution SEM. It was observed (1) that the GBM is composed of fibrillar network associated by granular materials, (2) that the fibrils are arranged to support mechanical stresses, and (3) that the nature and arrangement of the fibrils are various among different types of basement membranes in the kidney.(3) Structural change of the glomerulus : The structural change of the rat kidney after experimental procedures was analyzed morphob\metrically by means of light and transmission electron microscopy. It was found that the glomerular structure alters not so dramatically as expected from Kf change which was suggested by several physiological studies.

(1)肾小球的超微结构：采用冷脱水技术对大鼠肾脏进行TEM处理，以显示肾小球（包括系膜）的超微结构。肾小球系膜细胞具有许多突起，内含丰富的肌动蛋白丝。这些突起与肾小球基底膜（GBM）频繁接触，形成明显的锚，这种锚定作用被认为是产生血管壁张力以平衡毛细血管血压。(2)GBM的超微结构：冷脱水后用透射电镜或高分辨率扫描电镜观察GBM的超微结构。观察到（1）GBM由与颗粒材料相关的纤维状网络组成，（2）纤维排列以支持机械应力，和（3）纤维的性质和排列在肾中不同类型的基底膜中是不同的。(3)肾小球的结构变化：通过光学和透射电子显微镜对实验程序后大鼠肾脏的结构变化进行形态学分析。结果发现，肾小球结构的变化并不像一些生理学研究所认为的那样明显。

项目成果

坂井 建雄: "近位尿細管細胞." キッセイク-ル. 8(4). 15-16 (1990)

Takeo Sakai：“近端肾小管细胞。” 15-16 (1990)。

坂井 建雄: "腎臓(3) ーメサンギウム細胞." 臨床栄養. 76. 453 (1990)

Takeo Sakai：“肾脏 (3) - 系膜细胞。” 76. 453 (1990)。

Elger M. Sakai T, Kriz W: "Role of mesangial cell contraction in adaptation of the glomerular tuft to changes in extracellular volume." Pflugers Arch. 415. 598-605 (1990)

Elger M. Sakai T、Kriz W：“系膜细胞收缩在肾小球簇适应细胞外体积变化中的作用。”

Shibayama H, Sakai T, Yohro T: "Stapedius muscle and its motoneurons in the rat ーー A combined study of topographical anatomy and HRP transport experiments." 解剖学雑誌. 65. 120-133 (1990)

Shibayama H、Sakai T、Yohro T：“大鼠镫骨肌及其运动神经元 --- 地形解剖学和 HRP 转运实验的综合研究。解剖学杂志”65。120-133（1990）。

Sakai T: "Structure of the renal tubule and the new nomenclature of its segments." Nippon Rinsho. 47. 1451-1459 (1989)

Sakai T：“肾小管的结构及其节段的新命名法。”