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Study on the construction and diversity of actin filaments as supporting structure of the glomerular podocytes

肾小球足细胞支撑结构肌动蛋白丝的构建及多样性研究

基本信息

批准号：
10670030
负责人：
SAKAI Tatsuo
金额：
$ 2.05万
依托单位：
Juntendo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

1)Morphogenesis of podocytes and molecular architecture of slit membrane : We produced a monoclonal antibody (P-31) recognizing a new protein associating the intermediate filaments of podocytes (p250). We found bioochemically that the microtubule associating protein MAP4 found in variety of cells including podocytes is identical to MAP3 specific for neurons. We found that the monoclonal antibody 5-1-6 formerly produced at Niigata University (Kidney Research Institute) recognized rat-type nephrin which is consituent of slit diaphragm in the human beings.2) Characteristics of interstitium as supporting structure of glomerulus : To investigate contractility of tile mesangial cells and their ability to produce extracellular matrices, we analyzed electron microscopically the glomerular structure of null mutant mice for the gene of AT1 receptor for angiotensin II.We studied the constituent cells of renal interstitium as well as the renal tubular basement membrane which constitute the boundary between interstitium and parenchyme. In order to understand better the characteristics of renal interstitium, we studied the ultrastructtue of hepatic interstitium and found two populations of collagen fibrils which are produced most probably by fibroblasts and biliary epithelial cells, respectively.3) Evolution and development of renal vasculature : The kidney is structurally and functionally in close association with the vascular system. Its evolution and development is to be interpreted in the broad context of evolution and development of the vascular system. We studied the ultrastrucrure of vasculature in the stem villi of human placenta as model of developing blood vessels, and identified for the first time the arteries and veins within the stem villi after their ulstrastructural characterization. We reported also the charactresitics, development and evolution of renovascular system.

1)足细胞的形态发生和裂膜的分子结构：我们产生了一种单克隆抗体（P-31），识别一种与足细胞中间丝（p250）相关的新蛋白。我们在生物化学上发现，在包括足细胞在内的各种细胞中发现的微管相关蛋白MAP 4与神经元特异性的MAP 3相同。我们发现以前在新泻大学生产的单克隆抗体5-1-6（Kidney Research Institute）确认了大鼠型nephrin，其构成人类的裂隙隔膜。2）肾小球作为肾小球支持结构的特征：为了研究肾小球系膜细胞的收缩性及其产生细胞外基质的能力，我们对血管紧张素Ⅱ受体AT_1基因无效突变小鼠的肾小球结构进行了电镜分析。我们研究了肾小球的组成细胞以及构成肾小球和肾小管之间边界的肾小管基底膜。薄壁组织为了更好地了解肾间质的特点，我们对肝间质的超微结构进行了研究，发现了两类胶原纤维，它们最可能分别由成纤维细胞和胆管上皮细胞产生。3）肾血管系统的进化和发育：肾脏在结构和功能上与血管系统密切相关。它的进化和发展是在血管系统的进化和发展的广泛背景下解释的。本文以人胎盘为血管发育模型，研究了胎盘干绒毛血管的超微结构，并首次鉴定了干绒毛内的动脉和静脉。本文还报道了肾血管系统的特点、发展和演变。