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Preliminary Investigation for Inspection of Carcinogenic Mycotoxin Contamination in Foods and Herbal Drugs.

食品和中草药中致癌霉菌毒素污染检查的初步调查。

基本信息

批准号：
01571199
负责人：
SAKAI Tatsuo
金额：
$ 0.83万
依托单位：
Toyama Medical and Pharmaceutical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01571199/
关键词：
Mycotoxin Aflatoxin Aflatoxinogenic fungi Herbal drug Moutan bark Paeonol

项目摘要

Growth and toxin production of aflatoxinogenic fungi on major plant herbal drugs were investigated for inspection and survey of aflatoxin (AF) contamination in herbal drugs.Each sample powder or intact sample of herbal drugs was inoculated with aflatoxinogenic strains of aspergilli and incubated. Among sixty-two samples of herbal drugs examined, active fungal growth and marked production of AF were recognized on ten. These contain large quantities of starch and glucides or lipids. Thus, it was assumed that these herbal drugs susceptible to AF contamination.On the other twenty-one samples, the fungi did not grow actively. AF production was low or not detected, in spite of active fungal growth, on another thirty-one samples. These results suggest that many herbal drugs have inhibitory effect on the fungal growth and AF production. The methanol extract of twenty-five samples was added to a powdered medium designed for investigation of the fungal growth and AF production at various concentrations.In Moutan bark and Magnolia bark, the fungal growth and AF production were remarkably inhibited. In Phellodendron bark, Coptis Rhizome, and Glycyrrhiza, the fungal growth was active but AF production was reduced. From an extract of Moutan bark, paeonol was isolated as the inhibitory constituent. Magnolol and honokiol from Magnolia bark showed marked and almost complete inhibition, respectively, of the fungal growth and AF production. Berberine and glycyrrhetic acid did not inhibit fungal growth, but did inhibit AF production.In Perilla herb, the production of AF B group was much higher than that of AF G group. When the methanol extract of Perilla herb was added to the powdered medium, AF B level increased, while AF G level decreased dependent upon the concentration of the extract. Two active substances were isolated from Perilla herb and identified as dill apiole and elemicin. This effect was also found in myristicin, Known to be a constituent of Perilla herb.

为检测中草药中黄曲霉毒素（AF）的污染情况，对主要植物药上产黄曲霉毒素真菌的生长和产毒情况进行了研究。在62个样本的草药检查，积极的真菌生长和AF显着的生产被确认为10。它们含有大量的淀粉和糖类或脂类。因此，我们推测这些中草药易受AF污染。在其他21个样品上，真菌没有活跃地生长。AF生产低或未检测到，尽管活跃的真菌生长，在另外31个样品。这些结果表明，许多中草药对真菌生长和AF产生具有抑制作用。将25个样品的甲醇提取物添加到粉末培养基中，以研究真菌的生长和AF的产生，在厚朴和厚朴中，真菌的生长和AF的产生被显著抑制。在黄柏、黄连和甘草中，真菌生长活跃，但AF产量减少。从厚朴的提取物中分离出丹皮酚作为抑制成分。厚朴酚和厚朴酚从厚朴表现出显着的和几乎完全抑制，分别对真菌的生长和AF生产。小檗碱和大黄酸对真菌生长无抑制作用，但对AF的产生有抑制作用，紫苏中AF B组的产量明显高于AF G组。当紫苏的甲醇提取物加入到粉末培养基中时，AF B水平增加，而AF G水平下降，这取决于提取物的浓度。从紫苏中分离得到两种活性物质，分别鉴定为莳萝芹菜素和榄香素。这种效果也被发现在肉豆蔻，已知是一个组成的紫苏药草。