The new biological estimation for air pollution, indoor pollution and working environment

空气污染、室内污染和工作环境的新生物学估计

基本信息

批准号：
01570304
负责人：
KODAMA Yasushi
金额：
$ 1.41万
依托单位：
University of Occupational and Environmental Healt
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570304/
关键词：
Air Pollution Indoor Pollution EGF Cytochrome P-450 Nitrogen Dioxide AHH Cigarette Smoke PAH イソ酵素 Hela細胞

项目摘要

In this research project, we studied new biological indicators which were useful to assess the risk of chronic obstructive lung diseases and cancer promoting action by air pollution, indoor pollution and working environment. The biological effects of main pollutans, i. e. cigarette smoke, nitorgen dioxide (NO_2), benzo[a]pyrene (BaP), etc, were investigated in vivo and in vitro. Rats were exposed to side stream of cigarette smoke and NO_2 in an experimental chamber, 8 hours/day for 5 days. The activities of aryl hydrocarbon hydroxylase of lung, kidney and liver were increased by the exposure to cigarette smoke, but not by NO_2. Though total contents of cytochrome P-450 were not induced by either cigarette smoke or NO@_2, the dose-dependent inductions of cytochrome P-450b, c, d isozymes by the cigarette smoke wererecognized using Western Immunoblot analysis. The protein concentration, alkaline phosphatase and lactate dehydrogenase acticities in the bronchoalveolar lavage fluid (BALF) were also increased in the rats exposed to cigarette smoke or NO_2. The change of BALF was the most sensitive indicator that we examined in this study. The binding assay of epidermal growth factor (EGF) was also performed using HeLa cell and C3H/1OT1/2 cell. The binding of EGF to EGF receptor reached at a plateau 60-80 min after starting the incubation. This binding was varied by the density of cells on the plate. The plate with low density of the cells shows high binding capacity per cell number. We examined the effect of BaP on the EGF binding in the condition of the same density of the cells, however, it was not recognized that BaP inhibits EGF binding. In this experiment, some biological indicators did not show the result that we had expected. Further investigations were required to clarify the reason.

在该研究项目中，我们研究了新的生物学指标，这些指标对于评估慢性阻塞性肺部疾病的风险和通过空气污染，室内污染和工作环境促进癌症的风险。主要污染的生物学作用，即。 e。在体内和体外研究了香烟烟雾，二氧化碳（NO_2），苯并[A] pyrene（Bap）等。将大鼠暴露于香烟烟雾的侧面流，在实验室中，每天8小时，持续5天。暴露于香烟烟雾的情况下，增加了肺，肾脏和肝脏的芳基羟化酶的活性，而不是NO_2。尽管没有香烟烟雾或没有@_2诱导细胞色素P-450的总含量，但使用西方免疫印象分析对细胞色素p-450b，c，d同工酶的剂量依赖性诱导。在暴露于香烟烟雾或NO_2的大鼠中，还增加了支气管肺泡灌洗液（BALF）中蛋白质浓度，碱性磷酸酶和乳酸脱氢酶的作用。 BALF的变化是我们在这项研究中检查的最敏感的指标。还使用HeLa细胞和C3H/1OT1/2细胞进行表皮生长因子（EGF）的结合测定。 EGF与EGF受体的结合在开始孵育后60-80分钟时达到了高原。这种结合因板上细胞的密度而变化。细胞密度低的板显示每个细胞数的结合能力很高。我们检查了BAP在相同密度的条件下BAP对EGF结合的影响，但是，尚未认识到BAP抑制EGF的结合。在此实验中，一些生物学指标并未显示我们预期的结果。需要进一步调查以澄清原因。