Mechanism of Ethanol Degradation and Production in Intestinal Tract

乙醇在肠道中降解和产生的机制

• 批准号: 01570345
• 负责人: TANAKA Noriyuki
• 金额: $ 1.22万
• 依托单位: University of Occupational and Environmental Health, Japan (1990)Kanazawa Medical University (1989)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570345/
• 关键词: ethanol degradation; ethanol production; contents of intestinal tract; intestinal bacteria; antibiotics; Toxycology; Ethanol decomposition; Ethanol metabolism; Intestinal microorganism

In this study, the intestinal tract contents in rabbit were examined bacteriologically in vitro and in vivo to clarify the mechanism of ethanol metabolism in it.1. In vivo study, it was demonstrated that ethanol was degraded and produced in the contents of cecum, colon and rectum.2. Alcohol dehydrogenase participated in the ethanol degradation in cecum content. Catalase had little effect on the degradation.3. These enzymes were created by enteric bacteria, and affected by chloramphenicol and colistin in vivo.4. The ethanol degradation in cecum content accounted for 3.5% of all ethanol degradation in vivo.5. In vitro study, enteric bacteria was demonstrated to degrade and produce the ethanol.6. The ethanol degradation of enteric bacteria (E. Coli) in vitro was mediated by alcohol dehydrogenase and catalase.The enteric bacteria that had a ability to degrade the ethanol was inhibited to multiply by chloramphenicol, streptomycin and colistin. It was suggested that the decrease of ethanol degradation by these antibiotics was the result of the inhibition to multiply the enteric bacteria.

本研究对兔肠道内容物进行了体内外细菌学检测，以阐明IT1乙醇代谢的机制。体内实验表明，乙醇在盲肠、结肠和直肠的内容物中被降解和产生。乙醇脱氢酶参与了盲肠内容物中乙醇的降解。过氧化氢酶对降解率影响不大。这些酶是由肠道细菌产生的，在体内受到氯霉素和粘菌素的影响。盲肠内容物中的乙醇降解量占体内乙醇降解量的3.5%。在体外研究中，发现肠道细菌能降解和产生乙醇。在体外，乙醇脱氢酶和过氧化氢酶介导了肠杆菌(E.Coli)对乙醇的降解，氯霉素、链霉素和粘菌素抑制了具有乙醇降解能力的肠杆菌的增殖。推测这些抗生素对乙醇的降解作用是由于抑制了肠道细菌的繁殖所致。

项目成果

田中 宣幸: "家兎腸管内におけるアルコ-ル分解および産生のメカニズム" 日本法医学雑誌.

Nobuyuki Tanaka：“家兔肠道中酒精分解和产生的机制”日本法医学杂志。

Noriyuki Tanaka: "Studies on the ethanol metabolism in intestine of rabbit." Jpn J Legal Med.

Noriyuki Tanaka：“兔子肠道中乙醇代谢的研究。”