Metabolic Engineering for Brewer's Yeast by CRISPR-Cas to Reduce Ethanol Production for the Development of a Non Alcoholic Craft Beer

通过 CRISPR-Cas 对啤酒酵母进行代谢工程，减少乙醇产量，用于开发无酒精精酿啤酒

• 批准号: 500795-2016
• 负责人: Vandenbossche, Jamie
• 金额: $ 6.11万
• 依托单位: Camosun College
• 依托单位国家: 加拿大
• 项目类别: Applied Research and Development Grants - Level 2
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=692299
• 关键词: Metabolic; Engineering; Brewer; Yeast; CRISPR

Camosun College has been actively involved in applied research in the chemical and biochemical sciences over the past four years. The Applied Chemistry and Laboratory Sciences (ACLS) group at Camosun College assists local companies with the product development/refinement phase by providing high quality analytical data using optimized analytical methods tailored to companies' needs. The lab consists of modern instrumentation incorporating automated extraction techniques, detection software and chemical compound libraries. The ACLS group builds on the expertise and strengths offered in Camosun College's Applied Chemistry and Biotechnology (ACBT) and Associate of Science Degree in Biology programs. In the fall of 2015, Camosun was awarded funding from NSERC for a project to partner with Phillips Brewing and Malting Compnay, on "Short-Circuiting Yeast Metabolism for the Development of a Non-Alcoholic Craft Ale and its Chemical Evaluation by Gas Chromatography/Mass Spectrometry". The success of this project and partnership has led to a further commitment by Phillips Brewing and Malting Company to work with Camosun on the next iteration of this project entitled "Metabolic Engineering of Brewer's Yeast by CRISPR-Cas to Reduce Ethanol Production for the Development of a Non Alcoholic Craft Beer". The purpose of this project is an extension of a previously awarded NSERC Engage for Colleges Grant to explore the genetics underpinning yeast physiology during fermentation to manipulate alcohol production and deliver a commercially feasible low alcohol craft lager.

在过去的四年里，卡莫森学院一直积极参与化学和生物化学科学的应用研究。Camosun学院的应用化学和实验室科学（ACLS）小组通过使用根据公司需求量身定制的优化分析方法，提供高质量的分析数据，帮助当地公司进行产品开发/改进阶段。实验室由现代仪器组成，包括自动提取技术、检测软件和化合物库。ACLS小组建立在卡莫森学院应用化学和生物技术（ACBT）和生物学副学士学位课程提供的专业知识和优势之上。2015年秋天，Camosun获得了NSERC的资助，与Phillips Brewing and Malting company合作开展了一个项目，名为“缩短酵母代谢以开发无酒精精酿啤酒及其气相色谱/质谱化学评价”。该项目的成功和合作关系促使菲利普斯酿酒和麦芽公司进一步承诺与Camosun合作，进行该项目的下一个迭代，名为“通过CRISPR-Cas对啤酒酵母进行代谢工程，以减少乙醇产量，开发无酒精精酿啤酒”。该项目的目的是对先前授予的NSERC大学参与资助的延伸，该资助旨在探索发酵过程中酵母生理学的遗传学基础，以操纵酒精生产，并提供商业上可行的低酒精精酿啤酒。