Role of anti-DNA antibody and anti-cardiolipin antibody for the progression of vasculopathy in collagen diseases

抗 DNA 抗体和抗心磷脂抗体在胶原病血管病变进展中的作用

• 批准号: 01570348
• 负责人: KOIKE Takao
• 金额: $ 1.34万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570348/
• 关键词: systemic lupus erythematosus; anti-phospholipid antibody; anti-cardiolipin antibody; anti-DNA antibody; lupus nephritis; thrombosis; fetal loss; V region gene; 血管病変; 流・早産; ハイブリド-マ

To clarify the specificity of anticardiolipin antibody (aCL), we developed 18 monoclonal hybridoma aCL from SLE prone, MRL-1pr/1pr mice and the reactivity of these antibodies to phospholipids, DNA, epithelial cells (HEp-2 cells), platelets, endothelial cells, heparin, protein C and thrombomodulin was examined. All the monoclonal aCL reacted with phosphatidylserine and 11 of 18 also reacted with phosphatidylinositol. Three of 18 hybridoma aCL bound to both ssDNA and dsDNA, and 10 of 18 bound to ssDNA but not to dsDNA. The characteristics of antinuclear antibody were manifest for 10 of 18 monoconal aCL reacted with platelets. Three of 18 monoclonal aCL bound to vascular endothelial cells and these monoclonal aCL also had a strong reactivity to heparin. No monoclonal aCL showed reactivity to protein C or to thrombomodulin.

为阐明抗心磷脂抗体（aCL）的特异性，我们从SLE易感小鼠MRL-1 pr/1 pr中筛选出18株抗心磷脂抗体，并检测了这些抗体与磷脂、DNA、上皮细胞（HEp-2细胞）、血小板、内皮细胞、肝素、蛋白C和血栓调节蛋白的反应性。所有的单克隆ACL反应与磷脂酰丝氨酸和11的18也与磷脂酰肌醇。18个杂交瘤aCL中有3个与ssDNA和dsDNA结合，10个与ssDNA结合但不与dsDNA结合。18例单锥型ACL中有10例与血小板反应，具有抗核抗体特征。18个单克隆ACL中有3个与血管内皮细胞结合，这些单克隆ACL对肝素也有很强的反应性。没有单克隆ACL显示蛋白C或血栓调节蛋白的反应性。

项目成果

Kurasawa,K.,Koike,T.,Matsumura,R.,Takabayashi,K.,Tomioka,H.,Ito,I.,Yoshida,S.: "The immunosuppressive FKー506 prevents progression of diabetes in in NOD mice." Clin.Immunol.Immunopathol.57. 274-279 (1990)

Kurasawa, K.、Koike, T.、Matsumura, R.、Takabayashi, K.、Tomioka, H.、Ito, I.、Yoshida, S.：“免疫抑制性 FK-506 可预防 NOD 小鼠糖尿病的进展。”临床免疫病理学。57。274-279（1990）

小池 隆夫: "Annual Review 免疫,1990" 中外医学社, 382 (1990)

Takao Koike：“免疫学年度评论，1990”Chugai Igakusha，382（1990）

Shimada,K.,Koike,T.,Ichikawa,K.,Tsutsumi,A.,Takabayashi,K.,Tomioka,H.,Yoshida,S.: "IgG class antiーcardiolipin antibody as a possible marker for evaluating fetal risk in patients with systemic lupis erythematosus" J.Autommunity. 2. 843-849 (1989)

Shimada, K.、Koike, T.、Ichikawa, K.、Ttsutsumi, A.、Takabayashi, K.、Tomioka, H.、Yoshida, S.：“IgG 类抗心磷脂抗体作为评估胎儿风险的可能标志物系统性红斑狼疮患者”J.Autommunity. 2. 843-849 (1989)

Matsuura, E., Igarashi, Y., Fujimoto, M., Ichikawa, K., Koike, T.: "Anticardiolipin cofactor(s) and differential diagnosis of autoimmune disease." Lancet. 336. 177-178 (1990)

Matsuura, E.、Igarashi, Y.、Fujimoto, M.、Ichikawa, K.、Koike, T.：“抗心磷脂辅助因子和自身免疫性疾病的鉴别诊断。”

Tsutsumi, A., Koike, T., Sugiyama, T., Matsumura, R., Sueishi, M., Takabayashi, K., Tomioka, H., Yoshida, S.: "Protein losing entheropathy in systemic lupus erythematosus, mixed connective tissue disease and Sjogren's syndrome." Ann. Rheum. Dis.

Tsutsumi, A.、Koike, T.、Sugiyama, T.、Matsumura, R.、Sueishi, M.、Takabayashi, K.、Tomioka, H.、Yoshida, S.：“系统性红斑狼疮中的蛋白质丢失性肠病，混合型