Role of anti-DNA antibody and anti-cardiolipin antibody for the progression of vasculopathy in collagen diseases

为了阐明抗心磷脂抗体(ACL)的特异性，我们从SLE易感的MRL-1PR/1PR小鼠中制备了18株抗心磷脂抗体，并检测了这些抗体对磷脂、DNA、上皮细胞(Hep-2细胞)、血小板、内皮细胞、肝素、蛋白C和血栓调节蛋白的反应性。所有单抗均与磷脂酰丝氨酸反应，其中11例还与磷脂酰肌醇反应。18株杂交瘤中有3株能与单链DNA和双链DNA结合，10株能与单链DNA结合而不与双链DNA结合。18例单锥前交叉韧带中有10例与血小板反应，表现出明显的抗核抗体特征。18个单克隆性ACL中有3个与血管内皮细胞结合，这些单克隆性ACL对肝素也有较强的反应性。克隆性ACL对蛋白C和血栓调节蛋白均无反应性。

To clarify the specificity of anticardiolipin antibody (aCL), we developed 18 monoclonal hybridoma aCL from SLE prone, MRL-1pr/1pr mice and the reactivity of these antibodies to phospholipids, DNA, epithelial cells (HEp-2 cells), platelets, endothelial cells, heparin, protein C and thrombomodulin was examined. All the monoclonal aCL reacted with phosphatidylserine and 11 of 18 also reacted with phosphatidylinositol. Three of 18 hybridoma aCL bound to both ssDNA and dsDNA, and 10 of 18 bound to ssDNA but not to dsDNA. The characteristics of antinuclear antibody were manifest for 10 of 18 monoconal aCL reacted with platelets. Three of 18 monoclonal aCL bound to vascular endothelial cells and these monoclonal aCL also had a strong reactivity to heparin. No monoclonal aCL showed reactivity to protein C or to thrombomodulin.