The role of anticardiolipin antibodies on the development of thromboembolic manifestations in patients with collagen autoimmune disorders

抗心磷脂抗体（aCL）存在于系统性红斑狼疮（SLE）及相关结缔组织疾病患者的血清中。抗磷脂综合征这一术语已被提出来解释aCL与各种临床特征的相关性，包括动脉和静脉血栓栓塞事件，我们研究了胶原性疾病患者中ACL在血栓栓塞表现发展中的作用，并获得了以下结果：1）β 2-糖蛋白I（β 2-GPI）是检测aCL所必需的。2）β 2-GPI是一种糖蛋白，以50 KD的表观分子量迁移并形成称为“Sushi”结构域的独特结构。3）aCL识别改变的β 2-GPI结构，4）建立了一种新的酶免疫分析体系，用于检测aCL，并对该体系进行了初步的研究，结果表明，aCL识别的是β_2-GPI结构的改变，而这种改变是由于与磷脂或氧修饰的固相表面相互作用而引起的。

Anticardiolipin antibodies (aCL) are found in sera of patients with systemic lupus erythematosus (SLE) and related connective tissue diseades. The term, antiphospholipid syndrome, has been proposed to explain the association of aCL and variety of clinical features, including arterial and venous thromboembolic events, recurrent fetal loss and thrombocytopenia.We investigated the role of aCL on the development of thromboembolic manifestations in patients with collagen diseases and obtained the following results.1) beta2-glycoprotein I (beta2-GPI) is essential for the detection of aCL.2) beta2-GPI is a glycoprotein, migrates with an apparent molecular mass of 50KD and forms a unique structure termed "Sushi" domains.3) aCL recognize altered structure of beta2-GPI, but not CL itself.4) A new EIA system for the detection of aCL without CL has been established.Besed on these findings, we concluded that aCL recognize beta2-GPI structure altered by interacting with negatively changed phospholipids or an oxygene modified solid-phase surface.