Virological and Immunological Study for Prevention of Rotavirus Gastroenteritis.

预防轮状病毒胃肠炎的病毒学和免疫学研究。

• 批准号: 01570540
• 负责人: NAKATA Shuji
• 金额: $ 1.34万
• 依托单位: Sapporo Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570540/
• 关键词: Rotavirus gastroenteritis; Epidemiology; Rotavirus vaccine; Booster inoculation; Protective effect; Poliovirus vaccine; 干渉現象; 随伴免疫応答; RRVワクチン; 血清中和抗体反応; 交差免疫; 発症防御; 分子疫学

Epiodemiologic study for serotypes of rotaviruses detected in Sapporo showed the relative frequency of individual serotype by year. Serotypes 1 to 4 of rotaviruses were detected during consecutive 3 winter seasons. The immunity against at least four serotypes may be required to induced broad protection. Divergency of RNA pattern of rotaviruses collected in Sapporo suggested that rotaviruses were easily changed genetically.We have conducted the clinical trial of rhesus rotavirus vaccine MMU-18006 (RRV) (serotype 3) in a welfare nursery in Sapporo. RRV was safe and highly immunogenic. Heterotypic immune response to serotypes 1,2 and 4 of human rotaviruses after RRV inoculation was not enough even after booster inoculation. Based on the serological data RRV may be effective for serotype 3 rotavirus but not enough for other serotype of rotaviruses. During the study period an outbreak of gastroenteritis due to wild strain of serotype 1 rotavirus (RV-1) occurred in the study population. Infa … More nts aged from 0 to 25 monts were divided into three groups based on the vaccine history ; booster vaccine, one dose vaccine and control group. Almost all infants were infected with RV-1 whereas the protection against RV-1 gastroenteritis was seen more in vaccine groups, especially in booster group than in control group. There was significant relationship between asymptomatic infection and higher levels of pre-outbreak antibody titers against KU (serotype 1) but not RRV ; the protection against rotavirus infection was serotype specific. If we analyzed the data in individual case, these phenomena are not so clearly mentioned. The study for local immune response and epitope specific immune response against serotype 1 rotavirus should be needed to answer this problem.A clinical trial of the simultaneous oral administration of RRV and poliovirus vaccines was conducted in the same nursery. The interference between these two vaccines was not observed. The data showed the possibility for combined vaccination with rotavirus and poliovirus vaccines in future. Less

在札幌检测到的轮状病毒血清型的流行病学研究显示了各血清型的相对频率。在连续3个冬季检测到轮状病毒1 ~ 4型。可能需要针对至少四种血清型的免疫力来诱导广泛的保护。在札幌收集的轮状病毒RNA图谱的差异表明，轮状病毒容易发生遗传改变，我们在札幌的福利托儿所进行了恒河猴轮状病毒疫苗MMU-18006（RRV）（血清3型）的临床试验。RRV安全性高，免疫原性强。接种RRV后，即使加强接种，对人轮状病毒血清1、2和4型的异型免疫应答也不充分。根据血清学数据，RRV可能对血清3型轮状病毒有效，但对其他血清型轮状病毒不够。在研究期间，研究人群中发生了由血清1型轮状病毒（RV-1）野生株引起的胃肠炎暴发。Infa ...更多信息 根据疫苗接种史将0 ~ 25蒙茨龄的新生儿分为3组：加强疫苗组、单剂疫苗组和对照组。几乎所有的婴儿都感染了RV-1，而疫苗组对RV-1胃肠炎的保护作用更强，尤其是加强组。无症状感染与爆发前抗KU（血清1型）抗体滴度较高水平之间存在显著关系，但与RRV无关;对轮状病毒感染的保护具有血清型特异性。如果我们分析个案的数据，这些现象就没有那么清楚地提到。为了解决这一问题，需要研究针对血清1型轮状病毒的局部免疫应答和表位特异性免疫应答。未观察到这两种疫苗之间的干扰。这些数据显示了将来轮状病毒和脊髓灰质炎病毒疫苗联合接种的可能性。少

项目成果

Noriaki Adachi et al: "Transmissibility and immunogenicity of the rhesus rotavirus vaccine MMU-18006." Igakunoayumi. 151. 67-68 (1989)

Noriaki Adachi 等人：“恒河猴轮状病毒疫苗 MMU-18006 的传播性和免疫原性。”

足立 憲昭: "サル由来ロタウイルスワクチンMMUー18006株の伝播性および免疫原性に関する研究" 医学のあゆみ. 151. 67-68 (1989)

足立典明：“猴源轮状病毒疫苗 MMU-18006 株的传播性和免疫原性研究”，《医学史》151. 67-68 (1989)。

Shozo Urasawa et al: "Survey of human rotavirus serotypes in different locales in Japan by ELISA with monoclonal antibodies." Rinsho to uirusu. 17. 132-136 (1989)

Shozo Urasawa 等人：“通过单克隆抗体 ELISA 对日本不同地区的人类轮状病毒血清型进行调查。”

足立 憲昭: "ロタウイルスワクチンMMUー18006株の伝播性,免疫原性ならびにブ-スタ-接種効果に関する検討" 臨床とウイルス. 18. 102-114 (1990)

足立典明：“轮状病毒疫苗 MMU-18006 株的传播性、免疫原性和加强接种效果的研究”《临床与病毒学》18. 102-114 (1990)。

浦沢正三: "本邦7地域における下痢症患者便中ヒトロタウイルスの血清型別試験成績" 臨床とウイルス. 17. 132-136 (1989)

Shozo Urasawa：“日本七个地区腹泻患者粪便中人轮状病毒血清型的检测结果”《临床和病毒学》17. 132-136 (1989)。