Ultrastractural Studies on interrelationship between initial

取孕晚期小鼠的顶骨，用2.5%的戊二醛固定在0.2M的含或不含0.2%单宁酸的醋酸盐缓冲液中，再用含有1.5%亚铁氰化钾的1%四氧化三铯进行后处理。细胞内的板层结构与高尔基复合体、内质网和质膜相联系，而细胞外的板层结构位于成骨细胞的外围，靠近或包围着新合成的类骨基质。在固定在pH7.3但未经单宁酸处理的样品中也发现了MLS，然而，这些结构是泡状的，比MLS更难发现。用含0.1%孔雀石绿的戊二醛固定于pH6.0的骨标本，细胞内外均含有油滴，而不含MLS。它们的直径从0.1到1.2微米不等，在pH值为7.0的标本中很少看到片层结构。在pH值为7.3的标本中，MLS的化学性质尚未确定。初步发现，这些结构中的一些可能是由脂肪酸组成的。

Parietal bone fragments from fetal mice of late gestation were incised, and fixed with 2.5% glutaraldehyde in 0.2M cacodylate buffer with or without 0.2% tannic acid, and postfixed with 1% osmium tetroxide containing 1.5% potassium ferrocyanide.Electron microsoopic study of bone specimens showed the presence of multilamellar structures(MLS), with a periodicity of approximately 6nm, inside and outside osteoblasts in specimens fixed at pH7.3 with tannic acid. The lamellar structures with in cells were associated with Golgi complexes, endoplasmic reticulum and plasma membrane, while extracellular lamellar structures were located at the periphery of osteoblasts and near, sometimes surrounding, newly synthesiged osteoid matrix. MLS were also found in specimens fixed at pH7.3 but not treated with tannic acid These structures, however, were vesicular and more difficult to find than MLS. The bone specimens fixed at pH6.0 with glutaraldehyde containing 0.1% malachite green contained oildroplets, instead of MLS, inside and outside cells. These ranged in diam from 0.1 to 1.2um and lamellar structures were rarely seen in specimens fixed at pH7.0The chemical nature of MLS in specimens fixed at pH7.3 has not been determined. Preliminary findings suggest that some of these structures may be composed of fatty acids.