Ultrastractural Studies on interrelationship between initial

初始之间相互关系的超结构研究

• 批准号: 01571001
• 负责人: TAKAHASHI Tsuneo
• 金额: $ 1.41万
• 依托单位: Kanagawa Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01571001/
• 关键词: osteoblast; multilamellar structures; phaspholipids; fatty acids; triacylglyceride; transmission electron microscopy; 脂肪滴; 透過型電顕; 培養; 油滴; 石灰化; 軟骨細胞

Parietal bone fragments from fetal mice of late gestation were incised, and fixed with 2.5% glutaraldehyde in 0.2M cacodylate buffer with or without 0.2% tannic acid, and postfixed with 1% osmium tetroxide containing 1.5% potassium ferrocyanide.Electron microsoopic study of bone specimens showed the presence of multilamellar structures(MLS), with a periodicity of approximately 6nm, inside and outside osteoblasts in specimens fixed at pH7.3 with tannic acid. The lamellar structures with in cells were associated with Golgi complexes, endoplasmic reticulum and plasma membrane, while extracellular lamellar structures were located at the periphery of osteoblasts and near, sometimes surrounding, newly synthesiged osteoid matrix. MLS were also found in specimens fixed at pH7.3 but not treated with tannic acid These structures, however, were vesicular and more difficult to find than MLS. The bone specimens fixed at pH6.0 with glutaraldehyde containing 0.1% malachite green contained oildroplets, instead of MLS, inside and outside cells. These ranged in diam from 0.1 to 1.2um and lamellar structures were rarely seen in specimens fixed at pH7.0The chemical nature of MLS in specimens fixed at pH7.3 has not been determined. Preliminary findings suggest that some of these structures may be composed of fatty acids.

切开妊娠晚期胎鼠的顶骨碎片，用含或不含 0.2% 单宁酸的 0.2M 二甲胂酸缓冲液中的 2.5% 戊二醛固定，并用含 1.5% 亚铁氰化钾的 1% 四氧化锇进行后固定。骨标本的电子显微镜研究显示存在多层结构（MLS）， 周期性约为6nm，用单宁酸固定在pH7.3的标本中的成骨细胞的内部和外部。细胞内的层状结构与高尔基复合体、内质网和质膜相关，而细胞外的层状结构位于成骨细胞的外围，并且靠近（有时围绕）新合成的类骨基质。在 pH7.3 固定但未用单宁酸处理的样本中也发现了 MLS，但这些结构呈囊状，比 MLS 更难发现。用含0.1%孔雀石绿的戊二醛固定在pH6.0的骨标本细胞内外含有油滴，而不是MLS。这些直径范围为 0.1 至 1.2um，在 pH7.0 固定的样本中很少看到层状结构。pH7.3 固定的样本中 MLS 的化学性质尚未确定。初步研究结果表明，其中一些结构可能由脂肪酸组成。

项目成果

高橋 常男: "バイオサ-ファクタント" サイエンス・フォ-ラム, 13 (1990)

高桥恒夫：“Biosafactant”科学论坛，13（1990）

高橋常男: "人工肺サ-ファクタントを用いたタンニン酸のリン脂質固定効果について" 神奈川歯学. 23. 610-621 (1989)

Tsuneo Takahashi：“关于使用人工肺表面活性剂的单宁酸的磷脂固定效果”神奈川牙科 23. 610-621 (1989)。

Tsuneo Takahashi: "An electron microscopic study of multilamellar bodies in bone-forming cells (Part5)- Multi- lamellar bodies in glycogen area of a chondrocyte-" J. Jpn. Med. Soc. Biol. Interface. 24. (1992)

Tsuneo Takahashi：“骨形成细胞中多层体的电子显微镜研究（第五部分）-软骨细胞糖原区域中的多层体-” J. Jpn.

高橋 常男: "骨形成細胞にみられる多層膜構造物の電顕的観察(3)ー多層膜(ラメラ)構造物の出現に関する各種化学的固定剤の影響ー" 日本界面医学会雑誌. 22. 1-13 (1992)

Tsuneo Takahashi：“在成骨细胞中观察到的多层膜结构的电子显微镜观察（3） - 各种化学固定剂对多层（层状）结构外观的影响 -”日本界面医学会杂志 22 .1-13（ 1992）

樋田 寿子: "細胞界面の観察 ーラット・プレドニゾロンの影響ー" 日本界面医学会雑誌. 22. 29-37 (1991)

Hisako Hida：“细胞界面的观察 - 泼尼松龙对大鼠的影响”日本界面医学会杂志 22. 29-37 (1991)。