The study on role of TGF β in estrogen-induced endometrial carcinoma

TGFβ在雌激素诱发子宫内膜癌中作用的研究

• 批准号: 12671621
• 负责人: TAKAHASHI Tsuneo
• 金额: $ 2.11万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671621/
• 关键词: TGF β; endometrial carcinoma

We showed the gene expression of TGF β 1, TGF β 2, and TGF β 3 in the uterus stimulated by estrogen. To investigate the physiological role of these TGF β isoforms we did in situ hybridization and immunohistochemistry study. By these study the gene expression of TGF βs were recognized more potent in epithelium than stroma In addition, these gene expression was confirmed as protein expression by immunohistochemistry.If the protein appeared by estrogen had physiological action, a receptor must be present. And we showed the presence of the receptor that was necessary for biological action.In addition, these protein decreased when estrogen was administered, and it was suggested that these action were controlled at a receptor levelThe moleculer mechanism of expression of TGF β by estrogen is still unclear. However, gene expression by estrogen and protein expression and the presence of a receptor strongly suggest that these protein take an important role in action mechanism of estrogen.

在雌激素刺激下，子宫组织中有转化生长因子β1、转化生长因子β2和转化生长因子β3的基因表达。为了研究这些转化生长因子β亚型的生理作用，我们进行了原位杂交和免疫组织化学研究。通过这些研究发现，转化生长因子βS的基因在上皮中的表达比在间质中更强，免疫组织化学证实这些基因的表达是蛋白质的表达，如果雌激素产生的蛋白质具有生理作用，就必须存在受体。此外，给予雌激素后，这些蛋白减少，提示这些作用被控制在受体水平上。雌激素表达转化生长因子β的分子机制尚不清楚。然而，雌激素的基因表达和蛋白表达以及受体的存在强烈表明这些蛋白在雌激素的作用机制中发挥着重要作用。

项目成果

Hirahara F, Sumiyoshi Y, Yamanaka M, Sugawara M, Takahashi T, Mori C: "Fetal exposure to endocrine disruptor in Japanese pregnant women"Cong.Anom.. 43. 257 (2002)

Hirahara F、Sumiyoshi Y、Yamanaka M、Sukawara M、Takahashi T、Mori C：“日本孕妇的胎儿暴露于内分泌干扰物”Cong.Anom.. 43. 257 (2002)

Hirahara F., et al.: "Fetal exposure to endocrine disruptors in Japanese pregnant women"Cong.Anom.. 42. 257 (2002)

Hirahara F., et al.：“日本孕妇的胎儿暴露于内分泌干扰物”Cong.Anom.. 42. 257 (2002)