The study on mechanism for regulation of interaction between human decidua and villi.

人体蜕膜与绒毛相互作用调控机制的研究。

• 批准号: 09470364
• 负责人: TAKAHASHI Tsuneo
• 金额: $ 4.1万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470364/
• 关键词: growth factor; lactoferrin; decidua; villi; placenta; KGF; hCG; BeWo cell; KGF受容体; BeWo細胞; 胚-子宮内膜相互作用; GnRH; mRNA; オートクリン; パラクリン

To investigate the mechanism by which embryo-endometrial/decidual interaction, we analyzed the biological actions of local factors such as growth factor and lactoferrin in human endometrium/decidua and villi.KGF, the gene of which enhanced in secretory phase endometrium and decidua, stimulated hCG secretion, but did not affect ^3H-thymidine incorporation in BeWo cells, human choriocarcinoma cell line. KGF receptor mRNA was detected in villi and BeWo cells, indicating that the action of KGF on hCG secretion was mediated through KGF receptor.PDGF, which was also produced in decidual cells, had a strong cell proliferative effect on decidual cells.The immunohistochemical analysis of localization of lactoferrin in the human placenta revealed that it was prominent in the tissues with chorioamnionitis, suggesting lactoferrin in these tissues exerts an antiinflammatory effect.These results indicate that local factors such as growth factor and lactoferrin play an important role in the regulation of implantation, placentation, and maintenance of pregnancy by acting in an autocrine/paracrine fashion.

为了探讨胚胎-子宫内膜/蜕膜相互作用的机制，我们分析了生长因子和乳铁蛋白等局部因子在人子宫内膜/蜕膜和绒毛中的生物学作用，在分泌期子宫内膜和蜕膜中增强的KGF基因刺激hCG分泌，但不影响人绒毛膜癌细胞系BeWo细胞的^3 H-胸苷掺入。KGF受体mRNA在绒毛和BeWo细胞中均有表达，提示KGF对hCG分泌的作用是通过KGF受体介导的，PDGF也存在于蜕膜细胞中，对蜕膜细胞有较强的增殖作用。这些结果表明，局部因子如生长因子和乳铁蛋白通过自分泌/旁分泌方式在着床、胎盘形成和妊娠维持的调节中起重要作用。

项目成果

Ikeda M: "Gene expression of gonadotropin-releasing hormone, but its receptor in human endometrium and decidua." Mol.Cell Endocrinol. 135. 165-168 (1997)

Ikeda M：“促性腺激素释放激素的基因表达，但其受体存在于人类子宫内膜和蜕膜中。”

Matsui H: "Gene expression of Keratinocyte growth factor and its receptor in the human endometrium/decidua and chorionic villi." Endocrine J.44. 867-871 (1997)

Matsui H：“角化细胞生长因子及其受体在人子宫内膜/蜕膜和绒毛膜绒毛中的基因表达。”

Saji, M., Taga, M., Matsui, H., Suyama, K., Kurogi, K., Minaguchi, H.: "Gene expression and specific binding of platelet-derived growth factor and its effect on DNA synthesis in human decidual cells." Mol.Cell.Endocrinol. 132. 73-80 (1997)

Saji, M.、Taga, M.、Matsui, H.、Suyama, K.、Kurogi, K.、Minaguchi, H.：“血小板衍生生长因子的基因表达和特异性结合及其对人类 DNA 合成的影响

石川浩志: "妊娠中の羊膜・絨毛膜におけるラクトフェリンの免度組織学的検討" 横浜医学. 49. 17-22 (1998)

Hiroshi Ishikawa：“妊娠期间羊膜和绒毛膜中乳铁蛋白的免疫组织学研究”横滨医学，49. 17-22 (1998)。

Taga M: "Endometriosis Today" Parthenon Publishing, 478 (1997)

Taga M：“今天的子宫内膜异位症”帕台农神庙出版社，478（1997）