AntiーCD3 Monoclonal Antibody and IL-2 Induced Cytotoxicity of T Lymphocytes from Oral Cancer Patients.
抗 CD3 单克隆抗体和 IL-2 诱导口腔癌患者 T 淋巴细胞的细胞毒性。
基本信息
- 批准号:01571088
- 负责人:
- 金额:$ 1.34万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1989
- 资助国家:日本
- 起止时间:1989 至 1990
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
A Grest number of cells, which have high level of cytotoxic activity, are demanded for successful adoptive immunotherapy with Lymphokine-Activated Killer (LAK) cells. We attempted to make culture CD3-LAK cells using anti-CD3 monoclonal antibody and interleukin-2, and investigated how the proliferative response and cytotoxic activity of LAK cells (CD3・LAK) are affected by BRM (cytokines, OK432, etc.). In clinically, therapeutic effects of the combination of radiation therapy, chemotherapy and adoptive immunotherapy are expected, therefor, we investigated the effect of these therapy on CD3-LAK cells.1. Induction of CD3・LAK cells : High proliferative response and cytotoxic activity were induced from peripheral blood lymphocytes (PBL) by using anti-CD3 monoclonal antibody and IL-2.2. The effect of cytokines (rIFNgamma, rTNF alpha, rG-CSF, rIL-1 alpha, rIL-1beta) on CD3・LAKcells : Proliferative response of CD3・LAK cells increased by rTNFalpha at low concentration of rIL-2. Cytotoxic activity of these cells were not affected by any cytokines. At the first stage of CD・LAK induction, OK432 significantly enhanced cytotoxic activity of these cells.3. The effect of antineoplastic agent (CDDP, 5-FU, MTX, PEP, BLM, ADR) and irradiation (1.25, 2.5, 5.0, 10.0, 20.0Gy) on CD3・LAK cells : Proliferative response of CD3-LAK cells were suppressed by both antineoplastic agent and irradiation.Cytotoxic activiy of these cells were neither affected by antineoplastic agent nor irradiation.These results indicate that LAK induction using together anti-CD3 monoclonal antibody with rIL-2 is available for adoptive immunotherapy on oral cancer patients, and that therapeutic effects of adoptive immunotherapy in combination with chemotherapy and radiation therapy are expected.
成功的过继免疫治疗需要大量具有高水平细胞毒活性的LAK细胞。本实验尝试用抗CD 3单克隆抗体和白细胞介素2(IL-2)培养CD 3-LAK细胞,观察BRM(细胞因子、OK 432等)对LAK细胞增殖反应和细胞毒活性的影响。在临床上,放疗、化疗和过继免疫治疗的联合应用有望取得较好的疗效,为此,我们研究了这些治疗方法对CD 3-LAK细胞的影响. CD 3·LAK细胞的诱导:用抗CD 3单克隆抗体和IL-2.2诱导外周血淋巴细胞(PBL)产生高增殖反应和细胞毒活性。细胞因子(rIFN γ、rTNF α、rG-CSF、rIL-1 α、rIL-1 β)对CD 3·LAK细胞的作用:低浓度rIL-2时rTNF α可增强CD 3·LAK细胞的增殖反应。这些细胞的细胞毒性活性不受任何细胞因子的影响。在CD·LAK诱导的第一阶段,OK 432显著增强了这些细胞的细胞毒活性.杀菌剂的作用(CDDP、5-FU、MTX、PEP、BLM、ADR)和照射(1.25、2.5、5.0、10.0、20.0Gy)对CD_3·LAK细胞的杀伤作用:CD 3-T细胞的免疫应答LAK细胞的杀伤活性受照射和化疗剂的双重抑制,但不受化疗剂和放疗的影响,提示用化疗剂诱导LAK细胞是可行的。抗CD 3单克隆抗体与rIL-2联合应用可用于口腔癌患者的过继免疫治疗,并可期待过继免疫治疗联合化疗和放疗的治疗效果。
项目成果
期刊论文数量(21)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
TAKAHASHI, Yuzo: "Cytokines in treatment for oral cancer." The Journal of Stomatological Society, Japan. Vol. 56 No. 1. 186 (1989)
TAKAHASHI, Yuzo:“细胞因子治疗口腔癌。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
高橋 雄三: "口腔癌とBRM療法" 日口外誌. 35(6). 1690-1698 (1989)
Yuzo Takahashi:“口腔癌和 BRM 治疗”日本口腔医学杂志 35(6) (1989)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
高橋 雄三: "口腔癌とBRM療法" 日本口腔外科学会雑誌. 35. 1690-1698 (1989)
Yuzo Takahashi:“口腔癌和 BRM 治疗”日本口腔颌面外科学会杂志 35. 1690-1698 (1989)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
TAKAHASHI, Yuzo: "BRM in the treatment for oral cancer." Jpn. J. Oral. Maxillofac. Surg.Vol. 35 No. 6. 1690-1698 (1989)
TAKAHASHI, Yuzo:“BRM 在口腔癌治疗中的应用。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
TAKAHASHI, Yuzo: "Adoptive immunotherapy for oral cancer (Adoptiveーimmunotherapy with IL-2 activated autologous lymphocytes)" The Journal of the Stomatological Society, Japan. Vol. 57 No. 2. 354 (1990)
TAKAHASHI, Yuzo:“口腔癌的过继免疫疗法(使用 IL-2 激活的自体淋巴细胞的过继免疫疗法)”日本口腔医学会杂志第 57 卷第 2. 354 期(1990 年)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
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TAKAHASHI Yuzo其他文献
TAKAHASHI Yuzo的其他文献
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{{ truncateString('TAKAHASHI Yuzo', 18)}}的其他基金
Information presentation methods to ferment good experiences facilitating spontaneous and sustainable learning
信息呈现方法可以发酵良好的经验,促进自发和可持续的学习
- 批准号:
16K00717 - 财政年份:2016
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Basic study for developing supporting device to assist adequate hip rotation during stance work
开发支撑装置以协助站立工作期间充分旋转髋部的基础研究
- 批准号:
25350025 - 财政年份:2013
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Presentation factors for instantaneous cognition of non-verbal smooth closed figures
非语言平滑闭合图形瞬时认知的呈现因素
- 批准号:
21700142 - 财政年份:2009
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Development and practical use of teaching materials for wet-lab medical education with core curriculum
湿实验室医学教育核心课程教材的开发与实践
- 批准号:
16390148 - 财政年份:2004
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of medical education materials that stimulate learners' cerebration
开发激发学习者思维的医学教育材料
- 批准号:
14207101 - 财政年份:2002
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Analyses of anti-tumor immune responses in the sentinel lymph nodes from the patients with oral
口腔癌患者前哨淋巴结抗肿瘤免疫反应分析
- 批准号:
13470428 - 财政年份:2001
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular expression of biologically active substances produced from newborn larvae of Trichinella
旋毛虫新生幼虫产生的生物活性物质的分子表达
- 批准号:
12670229 - 财政年份:2000
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
A study on exocrine substances of Trichinella that induce dedifferentiation and transformation of infected muscle cell
旋毛虫外分泌物质诱导感染肌细胞去分化和转化的研究
- 批准号:
09670255 - 财政年份:1997
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Immuno-Gene Therapy for the Oral Squamous Cell Carcinoma using with Anti-Tumor Cytotoxic T-Lymphocytes.
使用抗肿瘤细胞毒性 T 淋巴细胞对口腔鳞状细胞癌进行免疫基因治疗。
- 批准号:
08457541 - 财政年份:1996
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Studies on the development of new transplantation and replantation of teeth, and its clinical trials.
新型牙移植、再植技术的发展及临床试验研究。
- 批准号:
06557109 - 财政年份:1994
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
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