Factors of Interaction between Osteoblasts and Bone Marrow Cells in Bone Remodeling

成骨细胞与骨髓细胞在骨重塑中相互作用的因素

• 批准号: 01571113
• 负责人: MITANI Hideo
• 金额: $ 1.34万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01571113/
• 关键词: Osteoblasts; Bone marrow Cells; Bone Remodeling; Co-Culture; Facs; ALPase Activity; MAC-1; LFA-1; coーculture; LFAー1抗原; 生理的骨改造機構; co-culture; Mac-1; LFA-1; ALPase活性

The purpose of this investigation was to reveal the physiological bone remodeling mechanism by means of cellular interaction phenomenon between osteoblasts (OB) and bone marrow cells (BM) in vitro.When OB ( 1X10^6 cells) obtained from 4 weeks old mouse long bones were co-cultivated by using millicell as separator in CO_2 incubator for 14 days, the number of BM was increased and the high incorporation of ^3H-thymidine into cellular DNA of BM was markedly recognized after 3 day incubation. However, the number of BM and uptake of ^3H-thymidine into BM did not increase in BM only cultivation. The increased BM were identified to have surface antigens of Mac-1 and LFA-1 by FACS analysis. But, ALPase activity of OBco-cultivated with BM was remarkably suppressed after 3 days incubation and reduced to one half of that of control after 5 days incubation. These results clearly indicated that both OB and BM produced some differential factors of the clonal growth of Mac-1 and LFA-1 positive cells from BM cells and suppression of ALPase of OB in co-cultivation with OB and BM. It was suggested that the factors produced from interaction between OB and BM might regulate the mechanism of physiological bone remodeling.

本研究旨在通过体外培养的成骨细胞（osteoblasts，OB）与骨髓细胞（bonemarrowcells，BM）之间的细胞相互作用现象，揭示生理性骨重建的机制从4周龄小鼠长骨中获得1 × 10^6个细胞，以millicell为分离剂，在CO_2培养箱中共培养14天，培养3天后，骨髓细胞数量增加，且骨髓细胞DNA中的^3H-胸苷掺入量明显增加。然而，在只培养BM的情况下，BM的数量和BM对^3 H-胸苷的摄取并没有增加。经流式细胞仪检测，骨髓中含有Mac-1和LFA-1的表面抗原。但与BM共培养的OB，在培养3天后，ALP活性明显受到抑制，培养5天后，ALP活性下降至对照的一半。这些结果清楚地表明，OB和BM在与OB和BM共培养时，都产生了Mac-1和LFA-1阳性细胞从BM细胞克隆生长的差异因子以及OB和BM对ALR的抑制。提示成骨细胞与骨基质相互作用产生的因子可能参与调节生理性骨重建的机制。

项目成果

千葉 美麗,清水 義之,三谷 英夫: "Mechanical stressによる骨改造機構における骨芽細胞と骨髄細胞の相互作用の解析" 日本矯正歯科学会雑誌. 48. 677 (1989)

Mirei Chiba、Yoshiyuki Shimizu、Hideo Mitani：“机械应力诱导的骨重塑机制中成骨细胞和骨髓细胞之间的相互作用分析”日本正畸学会杂志 48. 677 (1989)。

千葉美麗: "Mechanical stressによる骨改造機構の解明1.Compressive forceを負荷した新生ラット培養長管骨における破骨細胞の分化誘導について" 日本骨代謝学会雑誌. 7S. 211 (1989)

Mirei Chiba：“机械应力诱导的骨重塑机制的阐明1.在受压力的新生大鼠培养的长骨中诱导破骨细胞分化”日本骨代谢学会杂志211（1989）。

Chiba, M., Shimizu, Y., Simizu, Y., Mitani, H.: "A study of bone remodeling mechanism induced by mechanical stress. 1. Differentiation of osteoclasts induced by compressive force in newborn rat cultured long bone" J Bone Mine Metab. 8. 48 (1990)

Chiba, M.、Shimizu, Y.、Simizu, Y.、Mitani, H.：“机械应力诱导的骨重塑机制的研究。1.新生大鼠培养长骨中压缩力诱导的破骨细胞的分化”J Bone

Chiba,M.,Shimizu,Y.,Shimizu,Y.and Mitani,H.: "A study of bone remodeling mechanism induced by mechanical stress IーDifferentiation osteoclasts induced by copmressive forcein new bone rat cultured long boneー" J Bone and Mineral Metabolism. 8. 48 (1990)

Chiba, M., Shimizu, Y., Shimizu, Y. and Mitani, H.：“机械应力诱导的骨重塑机制的研究I－压缩力诱导新生骨大鼠培养长骨中的分化破骨细胞－” J Bone and矿物质代谢。8. 48 (1990)

清水義之: "Mechanical stressによる骨改造機構の解明II.骨改造機構における骨芽細胞と骨芽細胞の相互作用因子の解析" 日本骨代謝学会雑誌. 7S. 207 (1989)

Yoshiyuki Shimizu：“机械应力诱导的骨重塑机制的阐明II。骨重塑机制中成骨细胞和成骨细胞之间的相互作用因素的分析”日本骨代谢学会杂志207（1989）。