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A study on the role of nitric oxide in the response of periodontal tissue to mechanical stimuli

一氧化氮在牙周组织机械刺激反应中作用的研究

基本信息

批准号：
09470465
负责人：
MITANI Hideo
金额：
$ 5.18万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470465/
关键词：
Nitric Oxicide Periodontal Tissue Mechanical Stress Tooth Movement

项目摘要

Nitric oxide, a mediator of vascular function, neurotransmission, and immune system, has been recently shown to play an important role in adaptive response of bone to mechanical stress/strain. Periodontal tissue receives and adapts to various mechanical stimuli such as masticatory force and orthodontic force. Thus, it is possible that NO is a mediator of adaptive physiological response of periodontal tissue to these mechanical stimuli. In the present study, we tested this possibility both in vitro and in vivo.1) Effect of cyclic tension force on NO and prostaglandin EィイD22ィエD2 production and mRNA expression of their synthases in human periodontal ligament cells.Application of cyclic tension force to human periodontal ligament cells induced a rapid and transient increase in NO release in the culture medium. Endothelial isoform of NO synthase (NOS) mRNA expression decreased. Release of prostaglandin EィイD22ィエD2 time-dependently increased by the application of force. Cyclooxygenase-2 mRNA expression increased, whereas cyclooxygenase-1 mRNA expression did not change. These results suggest that NO and PGEィイD22ィエD2 are involved in the adaptive response of periodontal ligament cells to mechanical stress/strain.2) Effect of topical administration of inhibitors of NO synthase and spontaneous NO donors on orthodontic tooth movement in rats.Upper first molars of male 8-week-old Wistar rats were moved buccally for 21 days. The topical administration of L-NAME (10 mg/mL), a general inhibitor of NOS activity, caused a significant reduction of tooth movement. On the other hand, L-NIO (5 mg/mL), a selective inhibitor of inducible isoform of NOS, had no effect on tooth movement. There was a tendency toward a decrease in tooth movement in animals treated with NO donors (NOR4 and NOC). These results suggest that NO sythesized by constitutive isoforms of NOS has an important role in orthodontic tooth movement.

一氧化氮是一种调节血管功能、神经传递和免疫系统的介质，近年来被证明在骨骼对机械应力/应变的适应性反应中发挥着重要作用。牙周组织接受并适应各种机械刺激，如咀嚼力和正畸力。因此，NO可能是牙周组织对这些机械刺激的适应性生理反应的介体。在本研究中，我们在体外和体内验证了这一可能性：1)周期性张力对人牙周膜细胞NO及其合酶PGE2ィイD22ィエD2的产生和表达的影响。内皮细胞亚型一氧化氮合酶(NOS)mRNA表达降低。前列腺素E-ィイ-D22-ィエ-D2释放量随施力时间的增加而增加。环氧合酶-2mRNA表达增加，而环氧合酶-1mRNA表达无变化。这些结果表明，NO和PGEィイD22ィエD2参与了牙周韧带细胞对机械应力/应变的适应性反应。2)局部应用NO合酶抑制剂和自发的NO供体对大鼠正畸牙移动的影响。局部应用一氧化氮合酶活性的一般抑制剂L-NAME(10 mg/mL)，可显著减少牙齿移动。而选择性一氧化氮合酶诱导型异构体抑制剂L-NIO(5 mg/mL)对牙齿移动无明显影响。在没有供体(NOR4和NOC)治疗的动物中，牙齿移动有减少的趋势。这些结果提示，一氧化氮合酶结构性亚型合成的一氧化氮在正畸牙齿移动中起重要作用。