Pathogenicity of Pneumonia Virus of Nice (PVM) in Immunodeficient Rats.

尼斯肺炎病毒 (PVM) 在免疫缺陷大鼠中的致病性。

• 批准号: 01580045
• 负责人: SATO Hiroshi
• 金额: $ 1.28万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01580045/
• 关键词: Virus; Laboratory animal; Pneumonia; Mouse; Rat; Sero-epidemiological method; PVM; HVJ; HVJ; ウイルス感染; 呼吸器感染; SPF

[1] Indirect fluorescent antibody method was applied for a detection of pneumonia virus of mouse (PVM) antibody in colonies of laboratory animals in Japan. The results showed that the antibody exist in SPF rats with high incidence. The antibody was also detected in rabbits. However, PVM antibody wasnot demonstrated in the sera of mouse in Japan.These results suggested that PVM infection is present in laboratory rats rather than mice in Japan. This phenomenon was very different from western countries, and pointed out the importance of microbiological monitoring for PVM in facilities and breeders in our country.[2] A trial of virus isolation was resulted in positive The characteristics of new isolates (31N and so on) was the same as the NO. 15 ; standard-type of PVM. 31N was infected to mice (ICR, *, 4-weeks old) amd rats (wistar, *, 3 weeks-old) for an animal experimentation.In the results, 31N induced the consolidation in lung. Also, the strain induced interstitial pneumonia in mice and rats. The severity by the strain was heavier in mice than rats.As a result, the patho-histological changes in rats were very important to investigators of animal experimentation and management of animal facility.

[1] 日本采用间接荧光抗体法检测实验动物群体中小鼠肺炎病毒（PVM）抗体。结果表明，SPF大鼠体内存在该抗体，且发病率较高。在兔子身上也检测到了该抗体。然而，在日本小鼠的血清中并未发现PVM抗体。这些结果表明PVM感染存在于实验室大鼠而非日本小鼠中。这种现象与西方国家有很大不同，指出了我国设施和饲养场微生物监测对PVM的重要性。 [2]病毒分离试验结果呈阳性，新分离株（31N等）的特征与NO相同。 15；标准型PVM。将31N感染小鼠（ICR，*，4周龄）和大鼠（Wistar，*，3周龄）进行动物实验。结果，31N诱导肺实变。此外，该菌株还诱发小鼠和大鼠间质性肺炎。小鼠中该菌株的严重程度较大鼠为重。因此，大鼠的病理组织学变化对动物实验研究者和动物设施管理具有重要意义。

项目成果

光岡 知足: "獣医実験動物学" 川島書店, 321 (1990)

光冈智族：《兽医实验动物学》川岛书店，321（1990）

宮田 博規: "Isolation of PVM(pneumonia virus of mouse) in rats from Japanese colonies." EXPERIMENTAL ANIMAL.

Hironori Miyata：“从日本殖民地实验动物体内分离出 PVM（小鼠肺炎病毒）。”

Yoji Watanabe: "Replication of HVJ & PVM in macrophages originated from lung."

渡边洋二：“HVJ 的复制

渡辺 洋二: "Replication of HVJ & PVM in macrophages originated from lung." EXPERIMENTAL ANIMAL.

Yoji Watanabe：“HVJ 和 PVM 在源自肺的巨噬细胞中的复制。”

宮田 博規: "Prevalence of Pneumonia virus of mouse (PVM) infection in laboratory rats: Evaluation of serological methods." EXPERIMENTAL ANIMAL.

Hironori Miyata：“实验大鼠中小鼠肺炎病毒 (PVM) 感染的患病率：实验动物的血清学方法评估。”