Newly Developed Assaying Method for the Harmful Free Radicals and Active Oxygens Generating in Food Substance

新开发的食品物质中有害自由基和活性氧产生的测定方法

• 批准号: 01580079
• 负责人: YODA Binkoh
• 金额: $ 0.77万
• 依托单位: Koriyama Women's College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01580079/
• 关键词: Active oxygen; Free radical; Food; Aging; Carcinogenesis

This project pursues a way to develop a new detecting method of trace amiuntof active oxygen species and free radicals generating in food substances. For this purpose an extremely sensitive single photoelectron counting method was introduced to catch such photons coming out from singlet oxygen molecules and other excited molecular species. In general, though these active oxygens and free radicals exist only in very low concentration levels in food and other substances of our circumstances but are thought to play an important role in the aging process and the carcinogenesis of human being. Following results were obtained for these two research years.1. Single photoelectron counting system was introduced to overcome a technical difficulty in the detection of very weak light emission that comes from the transition of excited molecules in food substances.2. Ultraーweak light emissions of usual raw food materials were in very low levels. After heat denaturation meats, fishes and cheese changed to emit photons in very high levels. On the contrary the light emission from boiled rice and bread and bread stayed in low levels.3. Some amino acids also emitted light in higher levels after heat treatment. Tryptophan was the most prominent light emitter among the heat denatured amino acids.4. Oxygen was essential for these light emitting phenomena.5. Spectral analyses revealed the participation of singlet oxygen in these light emissions.6. Scavenger experiment specific for the existence of free radicals pointed out that free radicals are also concerned in an essential part of these light emissions.7. Newly developed specific enhancers of light emission showed that singlet oxygen and superoxide exist in these light emitting denatured materials.8. Extracts of fresh vegetable and fruit effectively inhibited these light emission from heated meats and denatured amino acids. Analysis of the component that inhibits light emission in these extracts is presently under way.

本课题旨在探索一种新的食品中产生的微量活性氧和自由基的检测方法。为此，提出了一种非常灵敏的单光电子计数方法来捕捉单线态氧分子和其他激发态分子发出的光子。总的来说，虽然这些活性氧和自由基只以极低的浓度存在于我们环境中的食物和其他物质中，但它们被认为在人类的衰老过程和致癌过程中起着重要作用。这两年的研究结果如下：介绍了单光电子计数系统，克服了检测食品中受激分子跃迁产生的极弱光发射的技术难题。普通食品原料的超弱光辐射水平很低。肉、鱼和奶酪经过热变性后，会释放出非常高水平的光子。相反，煮熟的米饭、面包和面包的光发射则保持在较低水平。一些氨基酸在热处理后也会发出更高水平的光。色氨酸是热变性氨基酸中最显著的光发射器。氧对于这些发光现象是必不可少的。光谱分析显示单线态氧参与了这些光发射。清除剂实验专门针对自由基的存在指出，自由基也是这些发光的重要组成部分。新开发的特异发光增强剂表明，这些发光变性材料中存在单线态氧和超氧化物。新鲜蔬菜和水果的提取物有效地抑制了加热肉类和变性氨基酸的光发射。目前正在对这些提取物中抑制发光的成分进行分析。

项目成果

N. Suzuki, K. Suetuna, S. Masiko, T. Nomoto, Y. Toya, B. Yoda and H. Inaba: "Chemiluminescence reaction rates of Cypridina lufiferin analogues with superpxide : A StermーVolmer competitive quenching experiment with superoxide dismutase." Chem. Express. 5(8

N. Suzuki、K. Suetuna、S. Masiko、T. Nomoto、Y. Toya、B. Yoda 和 H. Inaba：“Cypridina lufiferin 类似物与 superpxide 的化学发光反应速率：使用超氧化物歧化酶的 Sterm-Volmer 竞争性猝灭实验。 ” 化学快报 5(8

R.Saeki: "Low-level chemiluminescence of water-imbibed soybean seeds" Agric.Biol.Chem.53. 3311-3312 (1989)

R.Saeki：“吸水大豆种子的低水平化学发光”Agric.Biol.Chem.53。

N.Suzuki: "Chemiluminescence of Cypridina luciferin analogues:Oxidantーspecificity." Photomed.Photobiol.12. 753-756 (1990)

N.Suzuki：“Cypridina 荧光素类似物的化学发光：氧化剂特异性。”Photomed.Photobiol.12（1990）。

N. Suzuki, K. Ogawa, B. Yoda, T. Nomoto, Y. Tota and H. Inaba: "Oxodantーspecificity on chemiluminescence of Cypridina luciferin analogues." Chem. Express. 5(10). 753-756 (1990)

N. Suzuki、K. Okawa、B. Yoda、T. Nomoto、Y. Tota 和 H. Inaba：“Cypridina 荧光素类似物的化学发光特异性”，Chem. 753-756。 ）

N. Suzuki: "Chemiluminescence of luminol with singlet oxygen generated from rosbengal system." Chem. Express. 5. 505-508 (1990)

N. Suzuki：“鲁米诺与罗斯本加系统产生的单线态氧的化学发光。”