Variety improvement of cultivated plants in northeast area of Thailand through biotechnology

在过去三年的研究计划中，saga大学的八名成员访问了坤敬大学。孔敬大学的七名成员应邀到佐贺大学学习新技术。1)体外形态发生的生理生化分析；植物激素对模式植物“Torenis”离体器官发生和pharbitis花芽形成的控制机制2)植物组织培养改良技术的转移、电杂交体细胞杂交和细胞融合。3)对杂草根系DNA提取、纯化、分析、同工酶分析、体细胞杂交原质体融合、酚类化合物分析等技术进行培训。4)植物病毒单克隆抗体生产技术转移。5)泰国野生芋头来自萨拉布里（1种）、乌隆他尼（2种）、廊开（4种）和清迈（5种）。所有的芋头染色体均为二倍体（2n=28），除了农开的一个形式（2n=42）。6)佐贺大学转系大豆新品种、黄豆新品种、Williams与sj杂交大田栽培评价2.7)组培技术在泰国竹、木瓜及传统药材有用植物群体繁殖中的应用7)木瓜、大蒜及部分蔬菜病毒病发生情况实地调查。

In the past three years of this research project, eight members of saga University visited at khon kaen University. From khon kaen University, seven members was invited to saga University in order to learn the new techniques.1) Physiological and biochemical analysis of morphogenesis in vitro ; control mechanisms of phytohormenes for in vitro organogenesis in a model plant "Torenis" and floral bud formation of Pharbitis.2) Transfer of techniques on plant tissue culture for crop improvement, somatic hybridization by electrical hybridizer and cell fusion.3) Training the techniques on DNA extraction, purification and analysis, isozyme analysis, proto-plast-fusion by somatic hybridizer, and phenolic compound analysis from roots of weeds.4) Transfer of production technique for monoclonal antibody against plant viruses.5) Wild taros in Thailand were collected form Saraburi (aform), Udon Thani (2 forms), Nong Khai (4 forms) and Chiang Mai (5 forms). All chromosomes in the taros consisted of diploids (2n=28) except for a form from Nong khai (2n=42).6) Evaluation on field cultivation of some soybean varieties including transferred ones from Saga University and new soybeab variety, cross between Williams and SJ-2.7) Application of tissue culture techniques for mass propagation of bamboo, papaya and useful traditional plants in Thailand for crude drugs.7) Field survey on occurrences of virus diseases on papaya, garlic and some vegetables.