Variety improvement of cultivated plants in northeast area of Thailand through biotechnology

在本研究项目的过去三年中，佐贺大学的八名成员访问了孔敬大学。从孔敬大学邀请了7名成员到佐贺大学学习新技术：1）离体形态发生的生理生化分析;植物激素对模式植物“蓝猪耳”的体外器官发生和牵牛属的花芽形成的控制机制。2）植物组织培养技术的转移用于作物改良，3）培养DNA的提取、纯化与分析、同工酶分析、体细胞杂交仪原生质体融合、和杂草根中的酚类化合物分析。4）抗植物病毒单克隆抗体生产技术转让。5）从萨拉布里（Saraburi）采集泰国野生塔罗（aform）、乌东他尼（2种形式）、廊开府（4种形式）和清迈（5种形式）。芋头的染色体均为二倍体（2n=28），只有一个来自Nong khai（2n=42）。6）大豆新品种（包括佐贺大学的转育品种和大豆新品种（威廉姆斯与SJ-2杂交）的田间栽培评价。7）组织培养技术在竹子大量繁殖中的应用，7）对番木瓜、大蒜和部分蔬菜病毒病发生情况进行了实地调查。

In the past three years of this research project, eight members of saga University visited at khon kaen University. From khon kaen University, seven members was invited to saga University in order to learn the new techniques.1) Physiological and biochemical analysis of morphogenesis in vitro ; control mechanisms of phytohormenes for in vitro organogenesis in a model plant "Torenis" and floral bud formation of Pharbitis.2) Transfer of techniques on plant tissue culture for crop improvement, somatic hybridization by electrical hybridizer and cell fusion.3) Training the techniques on DNA extraction, purification and analysis, isozyme analysis, proto-plast-fusion by somatic hybridizer, and phenolic compound analysis from roots of weeds.4) Transfer of production technique for monoclonal antibody against plant viruses.5) Wild taros in Thailand were collected form Saraburi (aform), Udon Thani (2 forms), Nong Khai (4 forms) and Chiang Mai (5 forms). All chromosomes in the taros consisted of diploids (2n=28) except for a form from Nong khai (2n=42).6) Evaluation on field cultivation of some soybean varieties including transferred ones from Saga University and new soybeab variety, cross between Williams and SJ-2.7) Application of tissue culture techniques for mass propagation of bamboo, papaya and useful traditional plants in Thailand for crude drugs.7) Field survey on occurrences of virus diseases on papaya, garlic and some vegetables.