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A Flower-Inducing Substance of High Molecular Weight From Cultivated Plants

来自栽培植物的高分子量开花诱导物质

基本信息

批准号：
01560021
负责人：
TANAKA Osamu
金额：
$ 1.34万
依托单位：
Konan University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

Plants initiate flower buds under a variety of conditions, such as specific day-length, age, low temperature and various type of stress. Molecular mechanisms underlying flower-bud formation have not been yet, however, been clarified.We found that the induction or activation of some proteases, probably followed by the degradation of some proteins, is necessary for the induction of flowering in Lemna, suggesting that the products degradated from the proteins by the protease have a promotive effect on flower induction of Lemna. Therefore, we initiated an attempt to isolate and characterize the agent by methods generally used for purification of protein. As a results, we recently discovered a flower-inducing factor of high molecular weight (about 120 kDa). The high-molecular-weight substance could be extracted from soybean, cabbage, rice and pea. The substance was purified to homogeneity. It had characteristic of a polypeptide (a molecular weight of 22 kDa), with an amino-terminal sequence of Leu-Val-Gly-Asn-Thr, and induced formation of flower-buds of Lemna paucicostata 151 at the concentration of 10^<-10> M.At present, we are trying is to determine the order of amino acids in the active substance obtained from soybean, cabbage, rice and pea and investigating how the active substance is produced or activated in plant grown under various flower-bud-inducing conditions, for understanding the mechanism of flower induction at the molecular level. Finally, we investigate whether or not the active substance can control the flowering of cultivated plants.

植物在不同的条件下萌发花蕾，如特定的日照长度、年龄、低温和各种类型的胁迫。然而，花芽形成的分子机制尚未被阐明。我们发现，一些蛋白酶的诱导或激活，可能随后是一些蛋白质的降解，对于Lemna的诱导开花是必要的，这表明蛋白酶从蛋白质降解的产物对Lemna的诱导开花有促进作用。因此，我们开始尝试用通常用于蛋白质纯化的方法分离和表征该剂。因此，我们最近发现了一个高分子量（约120 kDa）的花诱导因子。该高分子量物质可从大豆、白菜、水稻和豌豆中提取。该物质经纯化后均质。有特性的多肽(22 kDa的分子量),伴Leu-Val-Gly-Asn-Thr序列,和诱导形成花蕾的浮萍属paucicostata 151浓度的10 ^ < -10 > M.At礼物,我们是决定氨基酸的顺序在活性物质从大豆、卷心菜、大米、豌豆和调查如何生产或激活活性物质在植物生长在各种flower-bud-inducing条件下,在分子水平上了解花的诱导机制。最后，我们研究了活性物质是否能控制栽培植物的开花。