Structure and Mechanism of Eukaryotic Transcription Regulation
真核生物转录调控的结构和机制
基本信息
- 批准号:10625407
- 负责人:
- 金额:$ 35.34万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-06-01 至 2026-03-31
- 项目状态:未结题
- 来源:
- 关键词:ATP HydrolysisAddressArchitectureBRF1 geneBindingBiochemicalBiogenesisCell NucleusCellsChemicalsChromosomesCodeComplexCore AssemblyCryoelectron MicroscopyDNADNA-Directed RNA PolymeraseDataElementsEnhancersEnzymesEukaryotaGTF2IRD1 geneGene ExpressionGene Expression RegulationGene ProteinsGenesGeneticGenetic TranscriptionGoalsHumanIn VitroInterferon-betaMass Spectrum AnalysisMediatorMolecularMolecular BiologyOrganismPolymeraseProcessProductionProtein BiosynthesisProteinsRNARNA Polymerase IIRNA chemical synthesisRNA, Ribosomal, 5SRNA, ribosomal, 25SRecyclingResolutionRibosomal RNASeriesSignal PathwaySignal TransductionStructureSystemTAF1 geneTechniquesTimeTranscription Factor TFIIATranscription Factor TFIIBTranscription Factor TFIIIATranscription Factor TFIIIBTranscription InitiationTranscription Initiation SiteTranscriptional ActivationTranscriptional RegulationTransfer RNAUntranslated RNAVisualizationYeastscell growthcrosslinkgenetic informationhuman diseaseinterdisciplinary approachnovelparticlepolypeptidepromoterrRNA Genesreconstitutionrecruittranscription factortranscription factor TFIIEtranscription factor TFIIFtranscription factor TFIIHtranscription factor TFIIICtranslocase
项目摘要
Summary
Transcription is the first step to read out the genetic information stored in the chromosome. In eukaryotes,
three multi-subunit RNA polymerases (Pols), Pol I, II, and III transcribe the 25S ribosomal RNA (rRNA),
protein-coding, and short non-coding RNAs such as transfer RNA (tRNA) and 25S rRNA genes, respectively. A
wealth of genetic and biochemical data accumulated over 50 years have uncovered most of the molecular
players involved and details of the intricate regulatory circuits. The recruitment and assembly of the initiation
complex at the promoter represent one of the key regulatory steps during gene expression. The Pol II initiation
machinery includes the activator-bound Mediator along with a series of general transcription factors (GTFs)
(TFIID, TFIIA, TFIIB, TFIIE, TFIIF, and TFIIH) that assemble into a ~4-megadalton (MDa) pre-initiation
complex (PIC) on core promoter DNA. On the other hand, the Pol III enzyme is recruited onto its promoter with
the help of corresponding GTFs (TFIIIC, TBP, BRF1, and B''). This proposal aims to investigate the
mechanism of transcription regulation by directly visualizing these transcriptional competent initiation
complexes using single-particle cryo-EM. Comparable pictures of these different but evolutionarily related
systems at this critical step of gene regulation will provide an unprecedented, comprehensive view of this key
process in the central dogma of molecular biology.
总结
转录是读出存储在染色体中的遗传信息的第一步。在真核生物中,
三种多亚基RNA聚合酶(Pos)Pol I、II和III转录25 S核糖体RNA(rRNA),
编码蛋白质的RNA和短的非编码RNA,如转运RNA(tRNA)和25 S rRNA基因。一
50多年来积累的大量遗传和生物化学数据揭示了大多数
参与者和复杂的监管电路的细节。新教徒的招募和集会
启动子上的复合物代表了基因表达过程中的关键调控步骤之一。Pol II启动
转录机制包括激活子结合的介体沿着一系列的通用转录因子(GTF)
(TFIID、TFIIA、TFIIB、TFIIE、TFIIF和TFIIH),其组装成约4兆道尔顿(MDa)的预起始
核心启动子DNA上的PIC复合物。另一方面,Pol III酶被募集到其启动子上,
相应GTF(TFIIIC、TBP、BRF 1和B“)的帮助。本提案旨在调查
通过直接观察这些转录活性起始,
复合物使用单粒子cryo-EM。这些不同但在进化上相关的
系统在基因调控的这一关键步骤将提供一个前所未有的,全面的看法,这一关键
在分子生物学的中心法则过程。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Truncated hemoglobin o of Mycobacterium tuberculosis: the oligomeric state change and the interaction with membrane components.
结核分枝杆菌的截短血红蛋白 o:寡聚状态变化及其与膜成分的相互作用。
- DOI:10.1016/j.bbrc.2004.02.170
- 发表时间:2004
- 期刊:
- 影响因子:3.1
- 作者:Liu,Chong;He,Yuan;Chang,Zengyi
- 通讯作者:Chang,Zengyi
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Yuan He其他文献
Yuan He的其他文献
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{{ truncateString('Yuan He', 18)}}的其他基金
Structure and Mechanism of Eukaryotic Transcription Regulation
真核生物转录调控的结构和机制
- 批准号:
10445554 - 财政年份:2022
- 资助金额:
$ 35.34万 - 项目类别:
Structure and Mechanism of Non-Homologous End Joining
非同源末端连接的结构和机制
- 批准号:
10546447 - 财政年份:2020
- 资助金额:
$ 35.34万 - 项目类别:
Molecular mechanism of NLRP3 inflammasome activation
NLRP3炎症小体激活的分子机制
- 批准号:
10158435 - 财政年份:2020
- 资助金额:
$ 35.34万 - 项目类别:
Molecular mechanism of NLRP3 inflammasome activation
NLRP3炎症小体激活的分子机制
- 批准号:
10612352 - 财政年份:2020
- 资助金额:
$ 35.34万 - 项目类别:
Structure and Mechanism of Non-Homologous End Joining
非同源末端连接的结构和机制
- 批准号:
10331036 - 财政年份:2020
- 资助金额:
$ 35.34万 - 项目类别:
Molecular mechanism of NLRP3 inflammasome activation
NLRP3炎症小体激活的分子机制
- 批准号:
10390480 - 财政年份:2020
- 资助金额:
$ 35.34万 - 项目类别:
ROLE OF NEK7 PROTEIN IN NLRP3 INFLAMMASOME ACTIVATION AND INFLAMMATION
NEK7 蛋白在 NLRP3 炎症小体激活和炎症中的作用
- 批准号:
9179168 - 财政年份:2017
- 资助金额:
$ 35.34万 - 项目类别:
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