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Development of Transformation Methods Suitable for Plant Breeding

Development of Transformation Methods Suitable for Plant Breeding
开发适合植物育种的转化方法
批准号：
02304016
负责人：
HIRAI Atsushi
金额：
$1.92万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1990
资助国家：
日本
项目状态：
已结题
起止时间：
1990 至 1991
来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02304016/
关键词：
TransformationParticle gunLaser injectionTi plasmidDirect gene transferCell fusionレ-ザプロセッサ直接遺伝子導入法受粉過程の遺伝子導入接木による形質転換

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中文摘要
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英文摘要
Many methods for introducing genes to plants are reported. However, the best method for plant breeding is different from each case. We examined seven transformation methods, and tried to recognize their characteristics. Particle guns were successfully used not only to observe the transient expression of introduced genes, but also to construct transgenic plants. The Laser injection is a new method for transformation and showed the transient expression of a marker gene. The gene transfer was also observed by the grafting method to some extent. Pollens treated with DNA were used to crossing and showed usable for transformation. Ti plasmid mediated gene transfer is popular, and we also found to be functional. Direct gene transfer by PEG was excellent to construct transgenic monocot plants such as rice. Asymmetric cell fusion was convenient to transfer a large amount of genetic information.
期刊论文(11)
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会议论文
Lee, H. Y. and T. Kameya: "Selection and characterization of a rice mutant to 5-methyltryptophan." Theor. Appl. Genet.82. 405-408 (1991)
Lee, H. Y. 和 T. Kameya：“5-甲基色氨酸水稻突变体的选择和表征。”
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H.Honda: "A simple and efficient method for identification of hybrids using nonradioactive rDNA as probe." Jpn.J.Breed.40. 339-348 (1990)
H.Honda：“一种使用非放射性 rDNA 作为探针来鉴定杂交体的简单而有效的方法。”
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Toriyama,K.,J.C.Stein.M.Nasrallah & J.Nasrallah: "transformation of Brassica oleracea with an S-locus gene from B.campestris changes the self-incompatibility phenotype." Theor.Appl.Genet.81:. 769-776 (1991)
鸟山，K.，J.C.斯坦，M.纳斯拉拉
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T.Yamashita,A.Iida and H.Morikawa: "Evidence that more than 90% of beta-glucuronidase-expressing cells after particle bombardment directly receive the foreign gene in their nucleus." Plant Physiol.97:. 829-831 (1991)
T.Yamashita，A.Iida％20和％20H.Morikawa：％20“证据％20％20比％2090％20更多％20％20％20β-葡萄糖醛酸酶表达％20细胞％20后％20颗粒％20轰击％20直接％20接收％20％
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共 11 条
    Rice Mitochondrial Genome : Studies on the complete sequence, expression and post transcriptional regulation.
    Molecular breeding for submergence tolerance in crops
    • 批准号：
      12556002
    • 项目类别：
      Grant-in-Aid for Scientific Research (B)
    • 资助金额：
      $8.64万
    • 财政年份：
      2000
    • 负责人：
      HIRAI Atsushi
    • 依托单位：
      The University of Tokyo
    Molecular breeding studies on crop fertility by expressional regulation of respiration-related genes.
    • 批准号：
      09556002
    • 项目类别：
      Grant-in-Aid for Scientific Research (B)
    • 资助金额：
      $9.09万
    • 财政年份：
      1997
    • 负责人：
      HIRAI Atsushi
    • 依托单位：
      The University of Tokyo
    Establishment of New Breeding Science by Biotechnology
    • 批准号：
      08306001
    • 项目类别：
      Grant-in-Aid for Scientific Research (A)
    • 资助金额：
      $12.29万
    • 财政年份：
      1996
    • 负责人：
      HIRAI Atsushi
    • 依托单位：
      The University of Tokyo