Development of Transformation Methods Suitable for Plant Breeding

开发适合植物育种的转化方法

• 批准号: 02304016
• 负责人: HIRAI Atsushi
• 金额: $ 1.92万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02304016/
• 关键词: Transformation; Particle gun; Laser injection; Ti plasmid; Direct gene transfer; Cell fusion; レ-ザプロセッサ; 直接遺伝子導入法; 受粉過程の遺伝子導入; 接木による形質転換

Many methods for introducing genes to plants are reported. However, the best method for plant breeding is different from each case. We examined seven transformation methods, and tried to recognize their characteristics. Particle guns were successfully used not only to observe the transient expression of introduced genes, but also to construct transgenic plants. The Laser injection is a new method for transformation and showed the transient expression of a marker gene. The gene transfer was also observed by the grafting method to some extent. Pollens treated with DNA were used to crossing and showed usable for transformation. Ti plasmid mediated gene transfer is popular, and we also found to be functional. Direct gene transfer by PEG was excellent to construct transgenic monocot plants such as rice. Asymmetric cell fusion was convenient to transfer a large amount of genetic information.

报道了许多将基因导入植物的方法。然而，植物育种的最佳方法因情况而异。我们检查了七种转换方法，并试图识别它们的特征。利用粒子枪技术不仅可以成功地观察外源基因的瞬时表达，而且可以用于转基因植株的构建。激光注射法是一种新的转化方法，显示了标记基因的瞬时表达。嫁接法也在一定程度上观察到了基因的转移。经DNA处理的花粉用于杂交，表明可用于转化。Ti质粒介导的基因转移是流行的，我们也发现是功能性的。PEG直接法是构建水稻等转基因单子叶植物的理想方法。不对称细胞融合便于大量遗传信息的传递。

项目成果

Lee, H. Y. and T. Kameya: "Selection and characterization of a rice mutant to 5-methyltryptophan." Theor. Appl. Genet.82. 405-408 (1991)

Lee, H. Y. 和 T. Kameya：“5-甲基色氨酸水稻突变体的选择和表征。”

H.Honda: "A simple and efficient method for identification of hybrids using nonradioactive rDNA as probe." Jpn.J.Breed.40. 339-348 (1990)

H.Honda：“一种使用非放射性 rDNA 作为探针来鉴定杂交体的简单而有效的方法。”

Toriyama,K.,J.C.Stein.M.Nasrallah & J.Nasrallah: "transformation of Brassica oleracea with an S-locus gene from B.campestris changes the self-incompatibility phenotype." Theor.Appl.Genet.81:. 769-776 (1991)

鸟山，K.，J.C.斯坦，M.纳斯拉拉

T.Yamashita,A.Iida and H.Morikawa: "Evidence that more than 90% of beta-glucuronidase-expressing cells after particle bombardment directly receive the foreign gene in their nucleus." Plant Physiol.97:. 829-831 (1991)

T.Yamashita，A.Iida％20和％20H.Morikawa：％20“证据％20％20比％2090％20更多％20％20％20β-葡萄糖醛酸酶表达％20细胞％20后％20颗粒％20轰击％20直接％20接收％20％

Hattori, K.: "Inheritance of carotenoid pigmentation in flower color of chrysanthemum." Japan. J. Breed.41. 1-9 (1991)

Hattori, K.：“菊花花色中类胡萝卜素色素的遗传。”