Development of Transformation System for Plant Mitochondria
Development of Transformation System for Plant Mitochondria
批准号:
06556002
负责人:
HIRAI Atsushi
金额:
$9.34万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (A)
财政年份:
1994
资助国家:
日本
项目状态:
已结题
起止时间:
1994 至 1996
中文摘要
线粒体是真核细胞中进行有氧代谢的能量传递部位。能量转导是通过线粒体膜上的几种人蛋白复合体进行的。这些复合体的遗传信息存储在核和线粒体基因组中。因此,为了提高线粒体的活性,必须同时对线粒体基因组和核基因组进行转化。构建了转化水稻线粒体DNA的质粒DNA,通过定点突变将线粒体编码的cob基因突变为抗霉素抗性的形式。将突变的cob基因与atp1启动子和atp6的3‘非翻译区一起克隆到pBSKS载体中。为了加速水稻线粒体中的复制,还将水稻线粒体中的B4等质粒连接到该质粒上。用粒子枪将线粒体转化质粒DNA导入水稻愈伤组织。然而,到目前为止还没有获得转化细胞。改进后的质粒的构建正在进行中。
英文摘要
Mitochondria are the sites of energy transduction in eukaryotic cells which carry out aerobic metabolism. Energy transduction is conducted by several kinds of peotein complexes in the mitochondrial membrane. Genetic information of these complexes are stored in the nuclear and mitochondrial genomes. Thus, mitochondrial as well as nuclear genome should be transformed in order to improve mitochondrial activity.Plasmid DNA was constructed to transform rice mitochondrial DNA.Mitichondrial coded cob gene was mutated to antimycin resistant form by the site directed mutagenesis. The mutated cob gene, together with promoter region of atpl and 3' non-translated region of atp6 were cloned into pBSKS+ plasmid. Plasmid like DNA,B4, in rice mitochondria was also connected to the plasmid in order to accelerate the replication of the plasmid DNA in rice mitochondria. This plasmid DNA for mitochondria transformation was introduced into rice calli by a particle gun. However, transformed cells were not obtained so far. Construction of the improved plasmid is in progress.
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Nishiwaki, S. et al.: "Structure of a gene for subunit 9 of NADH dehydrogenase (nad9) in rice mitochondria and RNA editing of its transcript." Plant Cell Physiol.36. 1135-1138 (1995)
Nishiwaki, S. 等人:“水稻线粒体中 NADH 脱氢酶 (nad9) 亚基 9 的基因结构及其转录物的 RNA 编辑。”
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通讯作者:
Miyata,S.et al.: "Polymorphic distribution and molecular diversification of mitochondrial plasmid-like DNAs in the genus Oryza." Jpn.J.Genet.70. 601-614 (1995)
Miyata,S.et al.:“稻属线粒体质粒样 DNA 的多态性分布和分子多样化。”
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Kadowaki,K.et al.: "Targeting presequence acquisiton after mitochondrial gene transfer to the nucleus occurs by duplication of existing targeting signal." EMBOJ.15. 6652-6661 (1996)
Kadowaki,K.等人:“线粒体基因转移到细胞核后,通过复制现有的靶向信号来获取靶向前序列。”
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通讯作者:
Nakazono, M.et al.: "Homologous recombination mediated by two palindromic repeated sequences in the mitochondrial genome of Oryza" Theor. Appl. Genet.91. 1-8 (1995)
Nakazono, M.等人:“Oryza 线粒体基因组中两个回文重复序列介导的同源重组”理论。
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Nakazono,M.et al.: "The CRTA motif is present in the promoters of mitochondrial genes of rice." Plant.Sci.(in press.).
Nakazono,M.等人:“CRTA 基序存在于水稻线粒体基因的启动子中。”
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共 25 条
Rice Mitochondrial Genome : Studies on the complete sequence, expression and post transcriptional regulation.
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批准号:13460002
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项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$9.86万
-
财政年份:2001
-
负责人:HIRAI Atsushi
-
依托单位:
Molecular breeding for submergence tolerance in crops
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批准号:12556002
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.64万
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财政年份:2000
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负责人:HIRAI Atsushi
-
依托单位:
Molecular breeding studies on crop fertility by expressional regulation of respiration-related genes.
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批准号:09556002
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$9.09万
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财政年份:1997
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负责人:HIRAI Atsushi
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依托单位:
Establishment of New Breeding Science by Biotechnology
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批准号:08306001
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$12.29万
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财政年份:1996
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负责人:HIRAI Atsushi
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依托单位:
Studies on Molecular Breeding by Functional Analysis of Rice Mitochondrial Genome
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批准号:05404007
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$21.44万
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财政年份:1993
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负责人:HIRAI Atsushi
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依托单位:
The dynamic aspect of genome structures in plants
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批准号:04304012
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项目类别:Grant-in-Aid for Co-operative Research (A)
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资助金额:$10.24万
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财政年份:1992
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负责人:HIRAI Atsushi
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依托单位:
The construction of plasmids suitable for searching important rice genes.
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批准号:03556002
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项目类别:Grant-in-Aid for Developmental Scientific Research (B)
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资助金额:$10.82万
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财政年份:1991
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负责人:HIRAI Atsushi
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依托单位:
Study on molecular breeding by the structural analysis of rice genome.
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批准号:02454035
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$4.42万
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财政年份:1990
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负责人:HIRAI Atsushi
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依托单位:
Development of Transformation Methods Suitable for Plant Breeding
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批准号:02304016
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项目类别:Grant-in-Aid for Co-operative Research (A)
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资助金额:$1.92万
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财政年份:1990
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负责人:HIRAI Atsushi
-
依托单位:
Development of a System for Identification of Hybrid Plants
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批准号:01860002
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项目类别:Grant-in-Aid for Developmental Scientific Research (B).
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资助金额:$3.52万
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财政年份:1989
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负责人:HIRAI Atsushi
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依托单位:
Studies on the molecular breeding model by expression vectors
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批准号:62440008
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项目类别:Grant-in-Aid for General Scientific Research (A)
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资助金额:$13.12万
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财政年份:1987
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负责人:HIRAI Atsushi
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依托单位:
Studies on the structure and expression of chloroplast genes
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批准号:58440001
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项目类别:Grant-in-Aid for General Scientific Research (A)
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资助金额:$17.28万
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财政年份:1983
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负责人:HIRAI Atsushi
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依托单位:
海外基金