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Development of Transformation System for Plant Mitochondria

Development of Transformation System for Plant Mitochondria
植物线粒体转化系统的开发
批准号:
06556002
负责人:
HIRAI Atsushi
金额:
$9.34万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (A)
财政年份:
1994
资助国家:
日本
项目状态:
已结题
起止时间:
1994 至 1996

项目摘要

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中文摘要
翻译
线粒体是真核细胞中进行有氧代谢的能量转导位点。能量传递是由线粒体膜上的几种蛋白复合物进行的。这些复合物的遗传信息储存在细胞核和线粒体基因组中。因此,为了提高线粒体的活性,线粒体和核基因组都需要进行转化。构建质粒DNA转化水稻线粒体DNA。采用定点诱变方法将线粒体编码cob基因突变为抗霉素抗性基因。将突变的cob基因与atpl的启动子区和atp6的3′非翻译区克隆到pBSKS+质粒中。水稻线粒体中的质粒如DNA B4也连接到质粒上,以加速水稻线粒体质粒DNA的复制。用粒子枪将线粒体转化质粒DNA导入水稻愈伤组织。然而,到目前为止,还没有获得转化细胞。改良质粒的构建正在进行中。
英文摘要
Mitochondria are the sites of energy transduction in eukaryotic cells which carry out aerobic metabolism. Energy transduction is conducted by several kinds of peotein complexes in the mitochondrial membrane. Genetic information of these complexes are stored in the nuclear and mitochondrial genomes. Thus, mitochondrial as well as nuclear genome should be transformed in order to improve mitochondrial activity.Plasmid DNA was constructed to transform rice mitochondrial DNA.Mitichondrial coded cob gene was mutated to antimycin resistant form by the site directed mutagenesis. The mutated cob gene, together with promoter region of atpl and 3' non-translated region of atp6 were cloned into pBSKS+ plasmid. Plasmid like DNA,B4, in rice mitochondria was also connected to the plasmid in order to accelerate the replication of the plasmid DNA in rice mitochondria. This plasmid DNA for mitochondria transformation was introduced into rice calli by a particle gun. However, transformed cells were not obtained so far. Construction of the improved plasmid is in progress.
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会议论文
Nishiwaki, S. et al.: "Structure of a gene for subunit 9 of NADH dehydrogenase (nad9) in rice mitochondria and RNA editing of its transcript." Plant Cell Physiol.36. 1135-1138 (1995)
Nishiwaki, S. 等人:“水稻线粒体中 NADH 脱氢酶 (nad9) 亚基 9 的基因结构及其转录物的 RNA 编辑。”
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Kadowaki,K.et al.: "Targeting presequence acquisiton after mitochondrial gene transfer to the nucleus occurs by duplication of existing targeting signal." EMBOJ.15. 6652-6661 (1996)
Kadowaki,K.等人:“线粒体基因转移到细胞核后,通过复制现有的靶向信号来获取靶向前序列。”
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Nakazono, M.et al.: "Homologous recombination mediated by two palindromic repeated sequences in the mitochondrial genome of Oryza" Theor. Appl. Genet.91. 1-8 (1995)
Nakazono, M.等人:“Oryza 线粒体基因组中两个回文重复序列介导的同源重组”理论。
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25
    Rice Mitochondrial Genome : Studies on the complete sequence, expression and post transcriptional regulation.
    Molecular breeding for submergence tolerance in crops
    • 批准号:
      12556002
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $8.64万
    • 财政年份:
      2000
    • 负责人:
      HIRAI Atsushi
    • 依托单位:
    Molecular breeding studies on crop fertility by expressional regulation of respiration-related genes.
    • 批准号:
      09556002
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $9.09万
    • 财政年份:
      1997
    • 负责人:
      HIRAI Atsushi
    • 依托单位:
    Establishment of New Breeding Science by Biotechnology
    • 批准号:
      08306001
    • 项目类别:
      Grant-in-Aid for Scientific Research (A)
    • 资助金额:
      $12.29万
    • 财政年份:
      1996
    • 负责人:
      HIRAI Atsushi
    • 依托单位:
    海外基金