Molecular breeding studies on crop fertility by expressional regulation of respiration-related genes.

通过呼吸相关基因的表达调控对作物育性进行分子育种研究。

• 批准号: 09556002
• 负责人: HIRAI Atsushi
• 金额: $ 9.09万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09556002/
• 关键词: respiration; gene expression; mitochondria; 低酸素; アルデヒド脱水素酵素; 稔性回復遺伝子; 核ゲノム; AOX; ミトコンドリアゲノム

Crop fertility depends on energy supply, and energy production is conducted by the respiration. We investigated the effect of oxygen on the expression of respiratory genes encoded in the nuclear and mitochondrial genomes of rice (Oryza sativa L.). Transcript levels of nuclear-encoded respiratory genes decreased by hypoxic treatment, whereas oxygen deprivation did not affect the expressions of mitochondrial-encoded respiratory genes. The effects of ruthenium red (an inhibitor of CaィイD12+ィエD1 fluxes from organelles) and/or CaClィイD22ィエD2 on plants under hypoxic conditions suggested that CaィイD12+ィエD1 is a physiological transducer of a low-oxygen signaling pathway for expression of the alternative oxidase gene (AOX1a), but not for expression of acetaldehyde dehydrogenase genes (ALDH2) and genes involved in the cytochrome respiratory pathway, in rice.

作物的肥力取决于能量的供应，而能量的生产是通过呼吸作用进行的。我们研究了氧对水稻（Oryza sativa L.）细胞核和线粒体基因组中呼吸基因表达的影响。低氧处理降低了核编码呼吸基因的转录水平，而缺氧并不影响核编码呼吸基因的表达。钌红的影响（细胞器中Ca ~（2+）D_1流的抑制剂）和/或CaCl ~（2+）D_2流D_2对低氧条件下植物的作用表明，Ca ~（2+）D_12 + D_1是交替氧化酶基因（AOX 1a）表达的低氧信号通路的生理转导子，但对水稻中乙醛脱氢酶基因（ALDH 2）和细胞色素呼吸途径相关基因的表达没有影响。

项目成果

M. Nakazono et al.: "Characterization of two cDNA clones encoding rice <@D1@>D2F<@D0@>D2-ATPase inhibitor proteins, both of which are targeted to mitochondria"planta. 210. 188-194 (2000)

M. Nakazono 等人：“编码水稻 <@D1@>D2F<@D0@>D2-ATP 酶抑制剂蛋白的两个 cDNA 克隆的特征，这两个克隆都靶向线粒体”。

D. Saisho et al.: "Characterization of the gene family for alternative oxidase from Arabidopsis thaliana."Plant Mol. Biol.. 35. 585-596 (1997)

D. Saisho 等人：“拟南芥替代氧化酶基因家族的表征。”植物分子。

Watanabe, A., M. Nakazono, N. Tsutsumi and A. Hirai: "AtUCP2 : a Novel Isoform of the Mitochondrial Uncoupling Protein of Arabidopsis thaliana."Plant Cell Physiol.. 40. 1160-1166 (1999)

Watanabe, A.、M. Nakazono、N. Tsutsumi 和 A. Hirai：“AtUCP2：拟南芥线粒体解偶联蛋白的新型异构体。”植物细胞生理学.. 40. 1160-1166 (1999)

Kanno, A., et al.: "Chloroplast derived trnH gene is expressed in the mitochondrial genome of gramineous plants." Plant Mol.Biol.34. 353-356 (1997)

Kanno, A. 等人：“叶绿体衍生的 trnH 基因在禾本科植物的线粒体基因组中表达。”

N. Kubo et al.: "Asingle nuclear transcript encoding mitochondrial RPS14 and SDHB of rice is processed by alternative splicing : Common use of the same mitochondrial targeting signal for different proteins."Proc. Natl. Acad. Sci. USA. 96. 9207-9211 (1999)

N. Kubo 等人：“编码水稻线粒体 RPS14 和 SDHB 的单个核转录本是通过选择性剪接进行处理的：不同蛋白质通常使用相同的线粒体靶向信号。”Proc.