Exposed cementum - morphology, characteristics and biological role in dental disease

暴露的牙骨质——形态、特征和在牙科疾病中的生物学作用

• 批准号: 02304052
• 负责人: HASEGAWA Kohji
• 金额: $ 4.99万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02304052/
• 关键词: Cementum; Exposed cementum; Periodontal disease; Endotoxin; Cell attachment; Root conditioning; Microhardness; Hypercallcification; ムコ多糖類; 内毒素; 機械的根面処理; ル-トプレ-ニング

The effects of periodontal and pulpal pathosis on cementum and the biological significance of cementum were investigated from various aspects. In addition, analysis on new cementum formation after periodontal surgery was performed histopathologically and clinically.As the results from X-ray microanalyzer study, the surface calcification of exposed cementum were observed, possibly by the influences from pocket environment. The scanning electron milcroscopical examination showed that the morphological changes did not give a constant feature and were very limited extent in the surface layers. Bacterial endotoxin was observed to be penetrated into the surface of the exposed cementum. Decrease in microhardness was seen to occur in the dentin under the exposed cementum. New cementum formation was delayed in non-vital teeth as compared to vital teeth.Adherence of cultured fibroblast cells in in vitro study was favorably observed when removal of exposed cementum in root planing procedure was limited only to the surface layer and there was remaining cementum. Furthermore, when dentin was exposed, the bacterial penetration from oral environment was observed through the dentinal tubules.Although some new observations concerning the characteristics of the cementum were established, several problems are still left for further study.

从多方面探讨了牙周病和牙髓病对牙骨质的影响及牙骨质的生物学意义。另外，对牙周手术后新牙骨质的形成进行了组织病理学和临床分析，X线显微分析结果显示，暴露牙骨质表面钙化，可能是牙周袋环境的影响。扫描电子显微镜检查表明，形态变化并没有给出一个恒定的功能，并在表面层非常有限的程度。观察到细菌内毒素渗透到暴露的牙骨质表面。暴露牙骨质下的牙本质显微硬度降低。与活髓牙相比，非活髓牙的新牙骨质形成延迟。在体外研究中，当根面平整术中暴露的牙骨质仅限于去除表层且有剩余牙骨质时，培养的成纤维细胞粘附良好。此外，在牙本质暴露的情况下，观察到口腔环境中的细菌通过牙本质小管侵入牙本质，虽然对牙骨质的特性有了一些新的观察，但仍有一些问题有待进一步研究。

项目成果

菅谷 勉,川浪 雅光,加藤 熈: "ポケット探針型超音波スケ-ラ-のNonーsurgical therapyへの応用" 日本歯周病学会会誌. 32. 134 (1990)

Tsutomu Sugaya、Masamitsu Kawanami、Hiroshi Kato：“袖珍探针型超声波洁牙机在非手术治疗中的应用”日本牙周病学会杂志 32. 134 (1990)。

K.Nishimura,Y.Noguchi and A.Yamaoka: "Cell attachment to diseased cementum in vitro." Journal of Periodontology. 62. 805-806 (1991)

K.Nishimura、Y.Noguchi 和 A.Yamaoka：“细胞在体外附着于患病牙骨质。”

上田 雅俊,稲田 芳樹,寺西 義浩,釜谷 晋平,北村 卓也,牧草 一人,白井 健雄,東 浩介,大橋 哲,大野 修一郎,山岡 昭,上村 参生,熨斗 秀光,神原 正樹,小西 浩二: "露出セメント質の表面性状について(その2)" 日本歯周病学会会誌. 32. 59 (1990)

上田正敏、稻田芳树、寺西义博、镰谷新平、北村拓也、草均仁、白井武夫、东浩介、大桥智、大野修一郎、山冈晃、上村佐野、能人英光、神原正树、小西浩二：“牙骨质暴露”表面性质（第 2 部分）”日本牙周病学会杂志. 32. 59 (1990)

R. Suda: "The penetration of Prophyromonas gingivalis LPS into the periodontally involved cementum." J. Japan Ass. Periodont.34. 103-112 (1992)

R. Suda：“牙龈卟啉单胞菌 LPS 渗透到牙周病牙骨质中。”

佃 宜和: "歯周疾患罹患セメント質に対する治療法の研究 I.ヒト歯肉由来培養線維芽細胞の付着・増殖による評価" 日本歯周病学会誌. 33. 90-100 (1991)

Yoshikazu Tsukuda：“受牙周病影响的牙骨质的治疗方法的研究 I. 通过培养的人牙龈来源成纤维细胞的附着和增殖进行评估”日本牙周病学会杂志 33. 90-100 (1991)。