Fundamental Study on the Induction and Prevention of Preleukemia Condition by Bovine Leukemia Virus

牛白血病病毒诱发和预防白血病前期的基础研究

• 批准号: 02556038
• 负责人: YOSHIKAWA Takashi
• 金额: $ 3.78万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02556038/
• 关键词: Bovine Leukemia Virus; Persistent Lymphocytosis; Bovine Leukemia; Initial Lesion; 初期病変; 初期変化; 前白血病病態; 前白血病期

Persistent lymphocytosis (PL) was experimentally prepared as a pre-leukemia condition and its pathological feature was studied from the viewpoint of virology, immunopathology and morphology.Experiment : As a donor, a cattle aged 2 years was used which responded positively to BLV antibody and had PL with 13, 300 lymphocytes/mul. Three calves aged 7-8 months (A, B and C) were intravenously inoculated with lymphocytes of 10^<10.3>, 10^9, 10^<9.6> respectively (second generation). Then, 10^<9.1>, 10^<9.1>, 10^<8.8> lymphocytes taken from the cattle "A"(positive to BLV antibody, 14, 868 lymphocytes/nul) were intravenously inoculated to each of another 3 cattle(a, b and c)(third generation). The experiment has been still being continues by using 2 animals inoculated with lymphocytes from the animal "a" (forth generation). These animals were investigated from the viewpoints of hematology, virology, immunology and histopathology.Results : (1) PL was induced in 2 animals of the second generation (A and B), 3 animals of the third generation (a, b and c), and 2 animals of the fourth generation 5-8 months after inoculation respectively. (2) With periphocytes, at weeks 1-2 after inoculation reversion of the ratio of T lyphocytes to B lymphocytes was recognized in all of the animals. The number of T lymphocytes (CD4, CD8) was decreased, while that of IgG positive B lymphocytes was increased. (3) Electron microscopy showed numerous virus particles in the peripheral lympocytes of the animals with PL which had been incubated for 24hrs. BLV antigens were detected in the cytoplasm when investigated by fluorescence antibody method using antiserum against BLV. (4) It was found that follicles of lymph nodes became larger in the 3 animals with PL (A, B and a) which were sacrificed at 6-9 months and that the constituent cells hd atypia. These cells sometimes responded positively to anti-tumor associated serum (CEBL-2)

实验制备了持续性淋巴细胞增多症（PL）作为白血病前期疾病，并从病毒学、免疫病理学和形态学角度对其病理特征进行了研究。实验：选用1头2岁、BLV抗体阳性、PL 13300淋巴细胞/头的牛作为供体。分别用10^<10.3>、10^9、10^<9.6>的淋巴细胞静脉接种3头7-8月龄犊牛（A、B、C）（第二代）。然后，从牛“A”（BLV抗体阳性，14868个淋巴细胞/nul）中提取10^<9.1>、10^<9.1>、10^<8.8>淋巴细胞，分别静脉接种给另外3头牛（A、b、c）（第三代）。实验仍在继续，用2只动物接种了第四代动物a的淋巴细胞。从血液学、病毒学、免疫学和组织病理学等方面对这些动物进行了研究。结果：(1)接种后5 ~ 8个月，第二代2只动物（A、B）、第三代3只动物（A、B、c）和第四代2只动物分别诱导了PL。(2)对于周围细胞，在接种后1-2周，所有动物的T淋巴细胞与B淋巴细胞的比例都出现了逆转。T淋巴细胞（CD4、CD8）数量减少，IgG阳性B淋巴细胞数量增加。(3)电镜观察发现，孵育24h后，外周血淋巴细胞内有大量病毒颗粒。用BLV抗血清荧光抗体法检测BLV抗原，在细胞质中检测到BLV抗原。(4) 6 ~ 9月龄处死的3只PL动物（A、B、A）淋巴结滤泡变大，组成细胞呈异型性。这些细胞有时对抗肿瘤相关血清（CEBL-2）有积极反应。

项目成果

Yoshikawa,H.et al: "Detection of Leukemia Viruses in Mammary Tissues affected by Mastitis in Bovine Leukemia Virus Antibody Carriers" Jpn.J.Vet.Sci.55. (1993)

Yoshikawa,H.等人：“牛白血病病毒抗体携带者受乳腺炎影响的乳腺组织中白血病病毒的检测”Jpn.J.Vet.Sci.55。

Yoshikawa,T.et al: "Pathology of Bovine Leukosis" Kitasato Arch.of Exp.Med.64. 101-114 (1991)

Yoshikawa,T.et al：“牛白血病病理学”Kitasato Arch.of Exp.Med.64。

Yoshikawa,T.et al: "Establishment and Maintenance of Bovine Leukosis virus-free Farm" Kitasato Arch.of Exp.Med.65. (1992)

Yoshikawa,T.et al：“牛白血病无病毒农场的建立和维护”Kitasato Arch.of Exp.Med.65。

Yoshikawa, Takashi et al: "Detection of Leukemia Viruses in Mammary Tissues affected by Mastitis in Bovine Leukemia Virus Antibody Carriers" Jpn. J. Vet. Sci.(1993)

Yoshikawa，Takashi 等人：“牛白血病病毒抗体携带者中受乳腺炎影响的乳腺组织中白血病病毒的检测”Jpn。

Yoshikawa,T.et al: "T/B lymphocyte ratio of Bovine Peripheral Lymphocytes in Experimental Persistent Lymphocytosis" Jpn.J.Vet.Sci.55. (1993)

Yoshikawa,T.et al：“实验性持续性淋巴细胞增多症中牛外周淋巴细胞的 T/B 淋巴细胞比率”Jpn.J.Vet.Sci.55。