Efects of Bordetella bronchiseptica Dermonecrotic Toxin

支气管败血博德特氏菌皮肤坏死毒素的作用

• 批准号: 05660352
• 负责人: YOSHIKAWA Takashi
• 金额: $ 1.34万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660352/
• 关键词: Atrophic rhinitis; Dermonecrotic toxin; Bordetella bronchiseptica; MC3T3-E1; Collagen; Alkaline phosphatase; Pig; Bordetella bronchiseptica; Pasteurella multocida; 豚萎縮性鼻炎; 壊死毒; 株化骨芽細胞

In pigs, atrophic rhinitis (AR) occurs as an upper respiratory disease caused principally by dermonecrotic toxins (DNT) of Bordetella bronchiseptica (Bb) . Turbinate atrophy is a characteristic lesion in AR.Histologically, there is a progressive loss of tubular cancellous bonein the turbinates. Severe degenerative changes in osteoblasts in the turbinates were observed by electron microscopy. The changes in osteocytes were less severe than those in osteoblasts.Osteoclasts appeared to be almost normal. Recently, a clonal MC3T3-E1 osteogenic cell line was established from newborn mouse calvaria, and has unique capacy to differentiate into osteoblasts in vitro (Kodama et al. 1981)The toxicity of Bb DNT on the structure and function of a MC3T3-E1 were investigated. The DNT induced a morphological change in the cells from a spindle shape to a spherical formwith many blebs. The toxintreated cells were viable and grew to form confluent cell layrs composed of irregularly shaped cells and multinuclear cells. The toxin inhibited elevation of alkaline phosphatase activity in the cells in a dose-dependent manner at concentrations from 1ng/ml to 1,000ng/ml. The accumulation of type 1 collagen in the cells was also reduced by the DNT.It is considered likely that Bb DNTimpairs the ability of the cells to defferentiate.

在猪中，萎缩性鼻炎（AR）作为主要由支气管败血波氏杆菌（Bb）的皮肤坏死毒素（DNT）引起的上呼吸道疾病而发生。鼻甲骨萎缩是变应性鼻炎的特征性病变。组织学上，鼻甲骨中管状松质骨逐渐丢失。电镜下可见成骨细胞发生严重的退行性改变。骨细胞的变化较成骨细胞轻，破骨细胞几乎正常。最近，从新生小鼠颅骨中建立了一个克隆的MC 3 T3-E1成骨细胞系，并具有独特的体外分化为成骨细胞的能力（Kodama等，1981）。DNT诱导细胞形态发生改变，由梭形变为球形，有许多泡。毒素处理的细胞存活，生长形成由不规则形细胞和多核细胞组成的融合细胞层。在1 ng/ml ~ 1, 000 ng/ml浓度范围内，毒素呈剂量依赖性地抑制细胞内碱性磷酸酶活性的升高。DNT还能减少细胞内Ⅰ型胶原的积累，这可能是由于Bb DNT能降低细胞分化能力。

项目成果

Takashi YOSHIKAWA et al: "Efects of Bordetella bronchiseptica Dermonecrotic Toxin on the Cytotoxity of Osteoblastic Clone MC3T3-E1 Cells" J Comp Pathol. 112. (1995)

Takashi YOSHIKAWA 等人：“支气管败血博德特氏菌皮肤坏死毒素对成骨细胞克隆 MC3T3-E1 细胞细胞毒性的影响”J Comp Pathol。

"Efects of Bordetella bronchiseptica Dermonecrotic Toxin on the Cytotoxity of Osteoblastic Clone MC3T3-E1 Cells" J Comp Path. 112. (1995)

“支气管败血博德特氏菌皮肤坏死毒素对成骨细胞克隆 MC3T3-E1 细胞细胞毒性的影响”J Comp Path。

Takashi YOSHIKAWA et al: "Toxicological study of Bordetella bronchiseptica dermonecrotic toxin on the Osteoblastic clone MC3T3-E1 cells" Journal of Veterinary Medical Science. 57. (1995)

Takashi YOSHIKAWA 等人：“支气管败血博德特氏菌皮肤坏死毒素对成骨细胞克隆 MC3T3-E1 细胞的毒理学研究”兽医医学杂志。