Enzyme immunoassay of human Mn-superoxide dismutase and its clinical application : Monitoring for myocardial injury.

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An enzyme-linked immunosorbent assay (ELISA) has been developed for human manganese-superoxide dismutase (Mn-SOD), using a specific monoclonal antibody raised against the purified enzyme. The MN-SOD molecule comprises four identical sub-units this permitted the development of a symmetrical assay, using the same monoclonal antibody as both capture and detector. The assay offers a specific, sensitive and convenient means of measuring immunoreactive MN-SOD in human sera. Under optimum conditions, the sensitivily of the assay permits the detection of 2-200 ng of purified MN-SOD from human liver. The mean serum Mn-SOD levels of normal healthy males and females were 99.8 <plus-minus> 24.8. (mean <plus-minus> SD) and 88.8 <plus-minus> 20.8 (mean <plus-minus> SD), respectively. Serum MN-SOD levels were determined in patients with acute myocardial infarction by ELISA. The serial determinations performed on 29 patients with acute myocardial infarction indicated that MN-SOD appears in serum in a biphasic manner ; an early small elevation was observed at approximately 16.2 (<plus-minus> 7.3) h and a high elevation at apporoximately 108 (<plus-minus> 20.6) h after the onset of symptoms. A negative correlation was found between the maximum value of the late elevation of MN-SOD and left ventricul