Enzyme immunoassay of human Mn-superoxide dismutase and its clinical application : Monitoring for myocardial injury.

人锰超氧化物歧化酶的酶联免疫分析及其临床应用：心肌损伤的监测。

• 批准号: 02557097
• 负责人: TANIGUCHI Naoyuki
• 金额: $ 6.66万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02557097/
• 关键词: Superoxide Dismutase; Enzyme Immumoassay; Myocardial Injury; Acute Myocardial Infarction; Cardiac Function; Tumor Necrosis Factor; ス-パ-オキサイドディマムタ-ゼ; MnーSuperoxide Dismutase; Myocardial infaraction; Tumor Necrosis Factor; Serum; Myocytes

An enzyme-linked immunosorbent assay (ELISA) has been developed for human manganese-superoxide dismutase (Mn-SOD), using a specific monoclonal antibody raised against the purified enzyme. The MN-SOD molecule comprises four identical sub-units this permitted the development of a symmetrical assay, using the same monoclonal antibody as both capture and detector. The assay offers a specific, sensitive and convenient means of measuring immunoreactive MN-SOD in human sera. Under optimum conditions, the sensitivily of the assay permits the detection of 2-200 ng of purified MN-SOD from human liver. The mean serum Mn-SOD levels of normal healthy males and females were 99.8 <plus-minus> 24.8. (mean <plus-minus> SD) and 88.8 <plus-minus> 20.8 (mean <plus-minus> SD), respectively. Serum MN-SOD levels were determined in patients with acute myocardial infarction by ELISA. The serial determinations performed on 29 patients with acute myocardial infarction indicated that MN-SOD appears in serum in a biphasic manner ; an early small elevation was observed at approximately 16.2 (<plus-minus> 7.3) h and a high elevation at apporoximately 108 (<plus-minus> 20.6) h after the onset of symptoms. A negative correlation was found between the maximum value of the late elevation of MN-SOD and left ventricul

本文报道了一种人锰超氧化物歧化酶（Mn-SOD）的酶联免疫吸附试验（ELISA）。MN-SOD分子包括四个相同的亚基，这允许使用相同的单克隆抗体作为捕获和检测器来开发对称测定。该方法提供了一种特异、灵敏、简便的测定人血清中免疫反应性MN-SOD的方法。在最佳条件下，该方法的灵敏度可达2-200 ng。正常人血清锰超氧化物歧化酶（Mn-SOD）水平为99.8 <plus-minus>± 24.8。(mean SD<plus-minus>）和88.8 <plus-minus>20.8（平均<plus-minus>SD）。采用酶联免疫吸附法测定急性心肌梗死患者血清MN-SOD水平。对29例急性心肌梗死患者进行的系列测定表明，血清中MN-SOD呈双相性，在症状发作后约16.2（7.3）h观察到早期小幅升高<plus-minus>，约108（20.6）h观察到高升高<plus-minus>。MN-SOD晚期升高最大值与左室肥厚呈负相关。

项目成果

Matsuda, Y.: "Human liver manganese superoxide dismutase : Purification and crystallization, subunit association and sulfhydryl reactivity." Eur. J. Biochem.194. 713-720 (1990)

Matsuda, Y.：“人肝锰超氧化物歧化酶：纯化和结晶、亚基缔合和巯基反应性。”

Deutsch,H.F.: "Preparation of human manganese superoxide dismutase by Tri-Phase partitioning and preliminary crystallographic data." J.Mol.Biol.219. 103-108 (1991)

Deutsch，H.F.：“通过三相分配和初步晶体学数据制备人锰超氧化物歧化酶。”

Taniguchi,N.: "Clinical significances of superoxide dismutases:Changes in aging,diabetes,ischemia and cancer." Advance in Clinical Chemistry. 29. (1991)

Taniguchi, N.：“超氧化物歧化酶的临床意义：衰老、糖尿病、缺血和癌症的变化。”

Kawaguchi,T.: "Serum Mn-superoxide dismutase:Normal values and increased levels in patients with acute myocardial infarction and several malignant diseases determined by enzyme-linked immunosorbent assay using a monoclonal antibody." J.Immunol.Methods. 12

Kawaguchi,T.：“血清锰超氧化物歧化酶：使用单克隆抗体通过酶联免疫吸附测定测定急性心肌梗塞和几种恶性疾病患者的正常值和升高水平。”

Kawaguchi, T.: "Serum Mn-superoxide dismutase : Normal values and increased levels in patients with acute myocardial infarction and several malignant diseases determined by enzyme-linked immunosorbent assay using a monoclonal antibody." J. Immunol. Method

Kawaguchi, T.：“血清锰超氧化物歧化酶：使用单克隆抗体通过酶联免疫吸附测定测定急性心肌梗塞和几种恶性疾病患者的正常值和升高水平。”