Inactivation of Cu,Zn-superoxide dismutase due to glycation

糖化导致铜、锌超氧化物歧化酶失活

• 批准号: 63480501
• 负责人: TANIGUCHI Naoyuki
• 金额: $ 4.48万
• 依托单位: Osaka University Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480501/
• 关键词: superoxide dismutase; Cu,Zn-SOD; glycation; active site liganding loop; Enzyme-linked immunosorbent Assay; retinopathy; Enzyme-linked Immunosorbent Assay; グリシト-ルリジン; スーパーオキサイドディスムターゼ; グリケーション; 不活性化

Human erythrocyte Cu,zn-superoxide dismutase comprises both glycated and non-glycated forms. In the cases of crude extracts as well as pure enzyme preparation obtained from erythrocytes of patients with Werner's syndrome, the glycated form was found in the bound fraction with a boronate column as in normal controls. However, the specific activity of the glycated form expressed as units per mg Cu,Zn-superoxide dismutase protein was significantly low and the enzyme was unstable as compared to the normal form. We conclude that in Werner's syndrome there is an increased amount of the inactive glycated form. We also found that glycation of Cu,Zn-SOD is highly associated with diabetic complication such as diabetic rdtinopathy or diabetic cataract.

人红细胞Cu，Zn-超氧化物歧化酶包括糖化和非糖化形式。在粗提物以及从维尔纳综合征患者的红细胞中获得的纯酶制剂的情况下，与正常对照一样，在具有硼酸盐柱的结合级分中发现了糖化形式。然而，比活性的糖化形式表示为单位每毫克铜，锌-超氧化物歧化酶蛋白是显着低，酶是不稳定的，相比正常形式。我们的结论是，在沃纳综合征有一个非活性糖化形式的增加量。我们还发现Cu，Zn-SOD的糖基化与糖尿病并发症如视网膜病变、白内障等密切相关。

项目成果

Taniguchi, N., Kinoshita, N., Arai, K., Iizuka, S., Usui, M., and Naito, T.: "Inactivation of erythrocyte Cu,Zn-superoxide dismutase through nonenzymatic glycosylation." The Maillard Reaction in Aging, Diabetes, and Nutrition. Alan R. Liss, Inc. 277-290,

Taniguchi, N.、Kinoshita, N.、Arai, K.、Iizuka, S.、Usui, M. 和 Naito, T.：“通过非酶糖基化灭活红细胞铜、锌超氧化物歧化酶。”

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Kinoshita, N.、Suzuki, S.、Matsuda, Y. 和 Taniguchi, N.：“通过甲胎蛋白抗体-凝集素酶免疫分析来表征糖链，用于肝脏疾病的研究。”

Taniguchi,N.: "Oxy-Radicals in Molecular Biology and Pathlolgy" Alan.R.Liss,inc., 15 (1988)

Taniguchi,N.：“分子生物学和病理学中的氧自由基”Alan.R.Liss,inc.，15 (1988)

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