Preparation and Properties of a Micro Enzyme Electrode for Creatine

肌酸微酶电极的制备及性能

• 批准号: 02640452
• 负责人: IKEDA Sanae
• 金额: $ 1.34万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02640452/
• 关键词: Creatine; Enzyme Electrode; Creatine Amidinohydrolase; Sarcosine Oxidase; 電極作製; 架橋化法

A micro enzyme electrode using creatine amidinohydrolase (EC 3.5.3.3) and sarcosine oxidase (EC 1.5.3.1) was developed for measuring creatine. The sensor consisted of platinum and silver wires fixed in a glass tubing with epoxy resin adhesive, and creatine amidinohydrolase and sarcosine oxidase immobilized on their tip by cross-linking method. The calibration curve for measurement of creatine, the effects of pH temperature, the amount of flavin adenine dinucleotide and concomitant compound on the sensitivity, and the life-time of the sensor were examined. Under optimal conditions, the response of the sensor was linear in the concentration of 10 muM - 0.65 muM creatine.

以肌酸脒基水解酶（EC 3.5.3.3）和肌氨酸氧化酶（EC 1.5.3.1）为电极材料，研制了一种测定肌酸的微酶电极。该传感器由铂丝和银丝固定在玻璃管中，用环氧树脂粘合剂固定在铂丝和银丝的尖端，用交联法固定肌酸脒基水解酶和肌氨酸氧化酶。测定了肌酸的校准曲线，考察了pH、温度、黄素腺嘌呤二核苷酸和共存化合物的量对传感器灵敏度的影响，以及传感器的寿命。在最佳条件下，传感器的响应在10 μ M- 0.65 μ M肌酸浓度范围内呈线性。