Planarity Recognition of Polycyclic Aromatic Hydrocarbons in Supercritical Fluid Chromatography

选择性是实现结构相关化合物分离以进行鉴定和定量的最重要变量之一。在液相色谱（LC）中，选择性可以通过改变移动的相组成、柱温和/或固定相的类型来控制。可以使用溶剂系统来改变LC中的选择性。然而，超临界流体色谱（SFC）中移动的相的选择非常有限;最常用的移动的相是二氧化碳，有时会向其中加入改性剂。虽然通过改变状态变量如温度和压力以及添加改性剂来修改选择性是可行的，但不会期望选择性发生大的变化。改变固定相的类型是获得理想选择性的最有效途径。使用填充毛细管SFC，可以使用在LC中提供不同选择性的各种类型的固定相。主要产品 ...更多信息 本研究的目的是研究使用相同固定相的LC和SFC之间选择性的差异，特别是关注它们识别多环芳烃分子平面性差异的能力。然后将在液相色谱中具有不同选择性的新型固定相在超临界流体色谱中进行考察，以了解液相色谱与超临界流体色谱的根本区别，结果表明，液晶键合相和环糊精键合相在分子平面识别方面具有比液相色谱更好的选择性，由于在超临界流体移动的相中溶剂化作用对溶质-固定相相互作用的影响小于液相色谱中的溶剂化作用，因此几乎是简单的分子-在色谱环境中可能产生分子间相互作用。基于分子间相互作用的新型固定相的设计可以很容易地在SFC中进行评估，而不是在LC条件下。所得信息可用于预测此类固定相在LC中的实际性能。设计新的固定相，使其对特定的分离具有更好的选择性，其设计方案是：设计分子间相互作用，考虑基于相互作用的固定相结构，合成固定相，用填充柱SFC评价性能，然后应用于LC。研究结果表明，该方法是获得高选择性液相色谱固定相的最佳方法之一。少

Selectivity is one of the most important variables to achieve the separation of structually related compounds for identification and quantitation. In Liquid chromatography (LC), selectivity can be controlled by changing the mobile phase composition, the column temperature and/or the type of stationary phases. It is possible to use solvent systems to change selectivity in LC. However, the choice of mobile phases in supercritical fluid chromatography (SFC) is very limited ; the most popular mobile phase is carbon dioxide, into which modifiers are sometimes added. Large changes in selectivity will not be expected, although it is feasible to modify the selectivity by changing the state variables such as temperature and pressure and adding mofifiers. The most effective approach to attain the desiable selectivity in SFC is to alter the type of stationary phases. With packed capillary SFC, various types of stationary phases which offer different selectivity in LC can be utilised. The main pur … More pose of this investigation is to examine the difference in selectivity between LC and SFC using identical stationary phases, especially focusing on their ability to recognize the difference in molecular planarity of polycyclic aromatic hydrocarbons. Then new stationary phases which have different selectivity in LC will be examined in SFC in order to know the basic difference between LC and SFC. As the results, we have found that liquid-crystal bonded phase and cyclodextrin bonded phase have better selectivity in molecular planarity recognition than those in LC, because in SFC mobile phase solvation effect to the solute-stationary phase interaction is smaller than that in LC situation and therefore almost simple molecular-molecular interaction is possible to make in chromatographic environment. The design of new stationary phases based on molecular-molecular interaction can be easily possible to evaluate in SFC, rather than LC conditions. Then the resulted information can be useful to predict the actual performance of such stationary phases in LC. The scheme of designing new stationary phases which can give us better of higher selectivity for particular separations is as follows : design the molecular-molecular interaction, consider the stationary phase structure based on the interaction, synthesize the phase, evaluate the performance by packed column SFC and then apply it in LC. The results of this investigation can clearly indicate this scheme is one of the best way to obtain highly selective stationary phases in LC. Less