AUTOMATED MICRO SEPARATION/ANALYSIS SYSTEM WITH FIBROUS MEDIA

带纤维介质的自动化微分离/分析系统

• 批准号: 14340233
• 负责人: JINNO Kiyokatsu
• 金额: $ 9.54万
• 依托单位: Toyohashi University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14340233/
• 关键词: MINIATURIZATION; SAMPLE PREPARATION; SOLVENT-LESS; FIBROUS MEDIUM; CHROMATOGRAPHY; NOVEL STATIONARY PHASE; TOTAL ANALYSIS SYSTEM; HIGH THROUGHPUT ANALYSIS; マイクロ分離システム; マイクロ固相抽出; キャピラリー分離; ファイバー媒体; 複合化システム; 自動化

MINIATURIZATION OF THE SAMPLE PREPARATION PROCESS AND THE SUBSEQUENT CHROMATOGRAPHIC SEPARATION PROCESS HAS BEEN REGARDED AS ONE OF THE KEY TECHNOLOGIES INCLUDING AN EFFECTIVE ON-LINE COUPLING OF THESE TWO MAJOR ANALYTICAL PROCESSES. COMPARED WITH TRADITIONAL SOLVENT-SOLVENT EXTRACTION," THE MINIATURIZED, SAMPLE PREPARATION TECHNIQUES COULD SIGNIFICANTLY REDUCE THE USAGE OF ORGANIC SOLVENTS AS WELL AS OTHER CHEMICALS. DOWNSIZING OF THE CHROMATOGRAPHIC SEPARATION SHOULD BE ACCOMPLISHED AT THE SAME TIME TO MAXIMIZE THE ADVANTAGEOUS FEATURE OF THESE MINIATURIZED SAMPLE PREPARATION METHODS.AS THE MINIATURIZED SAMPLE PREPARATION TECHNIQUE, THE AUTHORS HAVE DEVELOPED A NEW TYPE OF EXTRACTION METHOD, SO-CALLED "FIBER-IN-TUBE" SOLID-PHASE EXTRACTION (FIT-SPE), WHERE SEVERAL HUNDREDS OF FINE FILAMENTS OF SYNTHETIC FIBER WERE PACKED LONGITUDINALLY INTO A CAPILLARY. WITH THE FIT-SPE TECHNIQUE, AN AUTOMATED SAMPLE PREPARATION DEVICE HAS BEEN DEVELOPED AND THE APPLICATION FOR THE ANALYSIS OF TYPICA … More L COMPLEX SAMPLE MIXTURES SUCH AS ENVIRONMENTAL AND BIOLOGICAL SAMPLES. THE EXTRACTION CONDITIONS, SUCH AS EXTRACTION FLOWRATE AND TIME, TYPE OF FIBROUS MATERIALS USED, WERE SYSTEMATICALLY STUDIED AND ALSO SEVERAL PARAMETERS FOR THE AUTOMATION OF THE SAMPLE PREPARATION PROCESS WERE OPTIMIZED ALONG WITH THE DEVELOPMENT OF COMPUTER-CONTROLLING SYSTEM FOR THE EXTRACTION PROCESS.FIBER-PACKED SEPARATION COLUMNS HAVE BEEN INTRODUCED FOR THE SEPARATION OF THE ABOVE SAMPLES IN LIQUID CHROMATOGRAPHY (LC), CAPILLARY ELECTROCHROMATOGRAPHY (CEC) AND GAS CHROMATOGRAPHY (GC). THE SUCCESSFUL COMBINATION OF THE MINIATURIZED SAMPLE PREPARATION WITH FIBROUS EXTRACTION MEDIUM AND THE MICROSCALE SEPARATION WITH FIBROUS SEPARATION MEDIUM ENABLES THE DEVELOPMENT OF HIGH PERFORMANCE MICROSCALE ANALYTICAL SYSTEM HAVING A HIGHER EFFICIENCY, COST-EFFECTIVE FEATURES AND ENVIRONMENTALLY-SAFELY CHARACTERS. THE RESULTS OBTAINED IN THIS PROJECT HAVE BEEN PRESENTED WORLD-WIDE IN MANY INTERNATIONAL JOURNALS, AS FOUND IN THE ATTACHED PUBLICATION LIST, AND ALSO IN SEVERAL INTERNATIONAL MEETINGS AS PLENARY OR INVITED LECTURES. Less

样品制备过程和随后的色谱分离过程的小型化已被认为是关键技术之一，包括这两个主要分析过程的有效在线耦合。与传统的溶剂-溶剂萃取相比，小型化的样品制备技术可以显著减少有机溶剂和其他化学品的使用。同时要缩小色谱分离的规模，以最大限度地发挥这些小型化样品制备方法的优势。作为微型样品制备技术，作者开发了一种新型的提取方法，即所谓的“管中纤维”固相萃取（fit-spe），该方法将数百条合成纤维细丝纵向填充到毛细管中。摘要利用FIT-SPE技术，研制了一种自动化的样品制备装置，并将其应用于环境和生物样品等典型复杂样品混合物的分析。系统地研究了提取流量、提取时间、提取纤维材料类型等提取条件，并对样品制备过程自动化的几个参数进行了优化，同时开发了提取过程计算机控制系统。纤维填充分离柱可用于液相色谱（lc）、毛细管电色谱（cec）和气相色谱（gc）中上述样品的分离。纤维萃取介质的微型化样品制备与纤维分离介质的微尺度分离的成功结合，使高性能微尺度分析系统的开发具有更高的效率、成本效益和环保的特点。这个项目取得的成果已在世界各地的许多国际期刊上发表，如所附出版物清单所示，并在若干国际会议上作为全体会议或特邀演讲发表。少

项目成果

Y.saito, Y.Nkano, M.Imaizumi, Y.Morishita, Y.Kiso, K.Jinno: "Miniaturized Solid Phase Extraction as a Sample Preparation Technique for the Determination of Phthalates in Water"Analytical and Bioanalytical Chemistry. 373. 81-86 (2002)

Y.saito、Y.Nkano、M.Imaizumi、Y.Morishita、Y.Kiso、K.Jinno：“微型固相萃取作为测定水中邻苯二甲酸盐的样品制备技术”分析和生物分析化学。

Y.Saito: "Development of Miniaturized Sample Preparation with Fibrous Extraction Media"Journal of Chromatography A. 1025. 27-32 (2004)

Y.Saito：“使用纤维提取介质进行小型化样品制备的开发”《色谱杂志》A. 1025. 27-32 (2004)

Y.Saito, H.Ohta, K.Jinno: "Design and Characterization of Novel Stationary Phases Based on the Retention Behavior for Various Aromatic Compounds"Journal of Separation Science. 26(印刷中). (2003)

Y.Saito、H.Ohta、K.Jinno：“基于各种芳香族化合物的保留行为的新型固定相的设计和表征”《分离科学杂志》26（出版中）。

Y.Saito, M.Kawazoe, M.Imaizumi, Y.Morishita, Y.Nakao, K.Hatano, M.Hayashida, K.Jinno: "Miniaturized Sample Preparation and Separation Methods for environmental and Drug Analysis"Analytical Sciences. 18. 7-17 (2002)

Y.Saito、M.Kawazoe、M.Imaizumi、Y.Morishita、Y.Nakao、K.Hatano、M.Hayashida、K.Jinno：“环境和药物分析的小型化样品制备和分离方法”分析科学。

Y.Saito, M.Nojiri, M.Imaizumi, Y.Nakao, Y.Morishita, H.Kanchara, H.Matsuura, K.Kotera, H.Wada, K.Jinno: "Polymer-Coated Synthetic Fibers Designed for Miniaturized Sample Preparation Process"Journal of Chromatograohy 4. 975. 105-112 (2002)

Y.Saito、M.Nojiri、M.Imaizumi、Y.Nakao、Y.Morishita、H.Kanchara、H.Matsuura、K.Kotera、H.Wada、K.Jinno：“专为小型化样品设计的聚合物涂层合成纤维