AUTOMATED MICRO SEPARATION/ANALYSIS SYSTEM WITH FIBROUS MEDIA

样品制备过程和随后的色谱分离过程的小型化被认为是关键技术之一，包括这两个主要分析过程的连续在线耦合。与传统的溶剂-溶剂萃取法相比，”微型化的样品制备技术可以显著减少有机溶剂和其他化学品的使用。为了充分发挥这些微型样品前处理方法的优点，必须同时实现色谱分离的小型化.作为微型样品前处理技术，国外学者提出了一种新型的萃取方法，即所谓的“管中纤维”固相萃取（FIT-SPE），它是将几百根微细的纤维纵向包裹在毛细管中.用FIT-SPE技术研制了一种自动化样品前处理装置，并应用于典型样品的分析 关于我们 L复杂样品混合物，如环境和生物样品。对萃取流量、萃取时间、纤维材料种类等萃取条件进行了实验研究，并对样品制备过程自动化的几个参数进行了优化，同时开发了萃取过程自动化控制系统。毛细管电色谱（CEC）和气相色谱（GC）。纤维萃取介质微型化样品制备和纤维分离介质微尺度分离的成功结合，使高性能微尺度分析系统具有更高的效率、成本效益和环境安全特性。本项目所取得的成果已在世界各地的许多国际期刊上发表，见所附出版物清单，并在几次国际会议上作为全体会议或特邀演讲发表。少

MINIATURIZATION OF THE SAMPLE PREPARATION PROCESS AND THE SUBSEQUENT CHROMATOGRAPHIC SEPARATION PROCESS HAS BEEN REGARDED AS ONE OF THE KEY TECHNOLOGIES INCLUDING AN EFFECTIVE ON-LINE COUPLING OF THESE TWO MAJOR ANALYTICAL PROCESSES. COMPARED WITH TRADITIONAL SOLVENT-SOLVENT EXTRACTION," THE MINIATURIZED, SAMPLE PREPARATION TECHNIQUES COULD SIGNIFICANTLY REDUCE THE USAGE OF ORGANIC SOLVENTS AS WELL AS OTHER CHEMICALS. DOWNSIZING OF THE CHROMATOGRAPHIC SEPARATION SHOULD BE ACCOMPLISHED AT THE SAME TIME TO MAXIMIZE THE ADVANTAGEOUS FEATURE OF THESE MINIATURIZED SAMPLE PREPARATION METHODS.AS THE MINIATURIZED SAMPLE PREPARATION TECHNIQUE, THE AUTHORS HAVE DEVELOPED A NEW TYPE OF EXTRACTION METHOD, SO-CALLED "FIBER-IN-TUBE" SOLID-PHASE EXTRACTION (FIT-SPE), WHERE SEVERAL HUNDREDS OF FINE FILAMENTS OF SYNTHETIC FIBER WERE PACKED LONGITUDINALLY INTO A CAPILLARY. WITH THE FIT-SPE TECHNIQUE, AN AUTOMATED SAMPLE PREPARATION DEVICE HAS BEEN DEVELOPED AND THE APPLICATION FOR THE ANALYSIS OF TYPICA … More L COMPLEX SAMPLE MIXTURES SUCH AS ENVIRONMENTAL AND BIOLOGICAL SAMPLES. THE EXTRACTION CONDITIONS, SUCH AS EXTRACTION FLOWRATE AND TIME, TYPE OF FIBROUS MATERIALS USED, WERE SYSTEMATICALLY STUDIED AND ALSO SEVERAL PARAMETERS FOR THE AUTOMATION OF THE SAMPLE PREPARATION PROCESS WERE OPTIMIZED ALONG WITH THE DEVELOPMENT OF COMPUTER-CONTROLLING SYSTEM FOR THE EXTRACTION PROCESS.FIBER-PACKED SEPARATION COLUMNS HAVE BEEN INTRODUCED FOR THE SEPARATION OF THE ABOVE SAMPLES IN LIQUID CHROMATOGRAPHY (LC), CAPILLARY ELECTROCHROMATOGRAPHY (CEC) AND GAS CHROMATOGRAPHY (GC). THE SUCCESSFUL COMBINATION OF THE MINIATURIZED SAMPLE PREPARATION WITH FIBROUS EXTRACTION MEDIUM AND THE MICROSCALE SEPARATION WITH FIBROUS SEPARATION MEDIUM ENABLES THE DEVELOPMENT OF HIGH PERFORMANCE MICROSCALE ANALYTICAL SYSTEM HAVING A HIGHER EFFICIENCY, COST-EFFECTIVE FEATURES AND ENVIRONMENTALLY-SAFELY CHARACTERS. THE RESULTS OBTAINED IN THIS PROJECT HAVE BEEN PRESENTED WORLD-WIDE IN MANY INTERNATIONAL JOURNALS, AS FOUND IN THE ATTACHED PUBLICATION LIST, AND ALSO IN SEVERAL INTERNATIONAL MEETINGS AS PLENARY OR INVITED LECTURES. Less