AUTOMATED MICRO SEPARATION/ANALYSIS SYSTEM WITH FIBROUS MEDIA

样品制备过程和随后的色谱分离过程的小型化被认为是关键技术之一，包括这两个主要分析过程的有效在线耦合。与传统的溶剂-溶剂萃取相比，这种小型化的样品制备技术可以显著减少有机溶剂和其他化学品的使用量。作为一种小型化的样品制备技术，作者发展了一种新型的萃取方法，即所谓的“管内纤维”固相萃取(FIT-SPE)，将数百根细长的合成纤维纵向填充到毛细管中。利用FIT-SPE技术研制了一套自动制样装置，并将其应用于…的分析L更多的是混合环境样品和生物样品等复杂样品。对萃取流量、时间、纤维材料种类等萃取条件进行了系统的研究，并对样品制备过程自动化的几个参数进行了优化，开发了萃取过程的计算机控制系统。微型化样品制备与纤维萃取介质的成功结合和微尺度分离与纤维分离介质的成功结合，使高性能微型分析系统的开发具有更高的效率、性价比和环境安全性。该项目所取得的成果已在世界范围内的许多国际期刊上发表，如所附出版物清单所示，还作为全体会议或特邀讲座在几次国际会议上发表。较少

MINIATURIZATION OF THE SAMPLE PREPARATION PROCESS AND THE SUBSEQUENT CHROMATOGRAPHIC SEPARATION PROCESS HAS BEEN REGARDED AS ONE OF THE KEY TECHNOLOGIES INCLUDING AN EFFECTIVE ON-LINE COUPLING OF THESE TWO MAJOR ANALYTICAL PROCESSES. COMPARED WITH TRADITIONAL SOLVENT-SOLVENT EXTRACTION," THE MINIATURIZED, SAMPLE PREPARATION TECHNIQUES COULD SIGNIFICANTLY REDUCE THE USAGE OF ORGANIC SOLVENTS AS WELL AS OTHER CHEMICALS. DOWNSIZING OF THE CHROMATOGRAPHIC SEPARATION SHOULD BE ACCOMPLISHED AT THE SAME TIME TO MAXIMIZE THE ADVANTAGEOUS FEATURE OF THESE MINIATURIZED SAMPLE PREPARATION METHODS.AS THE MINIATURIZED SAMPLE PREPARATION TECHNIQUE, THE AUTHORS HAVE DEVELOPED A NEW TYPE OF EXTRACTION METHOD, SO-CALLED "FIBER-IN-TUBE" SOLID-PHASE EXTRACTION (FIT-SPE), WHERE SEVERAL HUNDREDS OF FINE FILAMENTS OF SYNTHETIC FIBER WERE PACKED LONGITUDINALLY INTO A CAPILLARY. WITH THE FIT-SPE TECHNIQUE, AN AUTOMATED SAMPLE PREPARATION DEVICE HAS BEEN DEVELOPED AND THE APPLICATION FOR THE ANALYSIS OF TYPICA … More L COMPLEX SAMPLE MIXTURES SUCH AS ENVIRONMENTAL AND BIOLOGICAL SAMPLES. THE EXTRACTION CONDITIONS, SUCH AS EXTRACTION FLOWRATE AND TIME, TYPE OF FIBROUS MATERIALS USED, WERE SYSTEMATICALLY STUDIED AND ALSO SEVERAL PARAMETERS FOR THE AUTOMATION OF THE SAMPLE PREPARATION PROCESS WERE OPTIMIZED ALONG WITH THE DEVELOPMENT OF COMPUTER-CONTROLLING SYSTEM FOR THE EXTRACTION PROCESS.FIBER-PACKED SEPARATION COLUMNS HAVE BEEN INTRODUCED FOR THE SEPARATION OF THE ABOVE SAMPLES IN LIQUID CHROMATOGRAPHY (LC), CAPILLARY ELECTROCHROMATOGRAPHY (CEC) AND GAS CHROMATOGRAPHY (GC). THE SUCCESSFUL COMBINATION OF THE MINIATURIZED SAMPLE PREPARATION WITH FIBROUS EXTRACTION MEDIUM AND THE MICROSCALE SEPARATION WITH FIBROUS SEPARATION MEDIUM ENABLES THE DEVELOPMENT OF HIGH PERFORMANCE MICROSCALE ANALYTICAL SYSTEM HAVING A HIGHER EFFICIENCY, COST-EFFECTIVE FEATURES AND ENVIRONMENTALLY-SAFELY CHARACTERS. THE RESULTS OBTAINED IN THIS PROJECT HAVE BEEN PRESENTED WORLD-WIDE IN MANY INTERNATIONAL JOURNALS, AS FOUND IN THE ATTACHED PUBLICATION LIST, AND ALSO IN SEVERAL INTERNATIONAL MEETINGS AS PLENARY OR INVITED LECTURES. Less