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Solventless Sample Preparation Method Using Supercritical or Subcritical Water as the Medium

以超临界或亚临界水为介质的无溶剂样品制备方法

基本信息

批准号：
12640586
负责人：
JINNO Kiyokatsu
金额：
$ 2.3万
依托单位：
Toyohashi University of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640586/
关键词：
supercritical fluid extraction solid phase extraction SPME SFE fiber-in-tube SPE sample preparation trace separation analysis environmental analysis miniaturization of analytical system 超臨界水亜臨界水無溶媒試料前処理

项目摘要

In the past ten years, supercritical fluid extraction (SFE) is being paid much attention as an analytical sample preparation technique, SFE provides shorter extraction time, which is a great reduction to Soxhlet extraction that may take days to perform, simple preconcentration steps, and most of all, significant reduction in the volumes of toxic and environmentally hazardous organic solvents used. However, in SFE some amounts of organic solvents are used as the collection media for the extracted analytes and as the modifier for the supercritical fluid. On the other hand, recent studies had also shown solid phase microextraction (SPME) to be fast, solvent free and an excellent performance technique. Similarly, SPME coupled to high performance liquid chromatography (HPLC) for non-volatile or thermally unstable compounds such as pesticides analysis also employs a small amount of organic solvent.For these reasons described above, this project has been intended to study the development of a solventless sample preparation method using supercritical or subcritical fluids as the extraction medium where SPME is a major extraction technique. As the results the analysis of pesticides in water has been made effectively by using SPME coupled with SFE with supercritical fluid as the medium and solventless sample preparation process has been realized by this technique. In the further development using supercritical water or subcritical water will improve the method developed in this project and this is the next target of this series of the projects.

在过去的十年中，超临界流体萃取（SFE）作为一种分析样品制备技术受到了广泛关注，SFE提供了更短的萃取时间，这大大减少了可能需要数天才能进行的索氏萃取，简单的预浓缩步骤，最重要的是，显着减少了有毒和环境有害的有机溶剂的使用量。然而，在超临界流体萃取中，一些量的有机溶剂被用作萃取分析物的收集介质和超临界流体的改性剂。另一方面，近年来的研究也表明固相微萃取（SPME）具有快速、无溶剂和性能优良等优点。同样地，SPME与高效液相色谱（HPLC）联用分析非挥发性或热不稳定化合物（如农药）也需要使用少量有机溶剂。基于上述原因，本项目旨在研究开发一种以SPME为主要萃取技术的、使用超临界或亚临界流体作为萃取介质的无溶剂样品制备方法。结果表明，以超临界流体为介质，SPME-SFE联用技术可以有效地分析水中的农药，实现了无溶剂样品前处理过程。在进一步的开发中，使用超临界水或亚临界水将改进本项目中开发的方法，这是本系列项目的下一个目标。