Dry artificial seeds through induction of desiccation tolerance of somatic embryos by abscisic acid and study of mechanism of the desiccation tolerance

脱落酸诱导体细胞胚耐干燥的干燥人工种子及其耐干燥机制研究

基本信息

  • 批准号:
    02660002
  • 负责人:
  • 金额:
    $ 1.54万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1990
  • 资助国家:
    日本
  • 起止时间:
    1990 至 1992
  • 项目状态:
    已结题

项目摘要

Artificial seed technology may provide potential benefits in plant propagation systems. If somatic embryos could maintain their viability after their water content was decreased to the level of true seeds, they were used as a method of germplasm storage and a model system of study of developmental plant biology as well as a utilization of artificial seed. The objective of this study was to investigate induction of desiccation tolerance in microspore-derived embryos of Brassica crops by exogenous application of ABA and to analyze the proteins and genes of the desiccation tolerant embryos.After desiccation treatment, the relative water content of embryos had decreased to about 10%. Desiccation tolerance of the embryos was induced by ABA. These phenomena were influenced by the size of embryos and the concentration of ABA, but not dependent on genotypes. The frequency of plant conversion of dry cotyledonary embryos which were treated with 10^<-4>-10^<-5>M ABA was 41-53%. In contrast, almos … More t all embryos lost their viability after desiccation, when they were not treated with ABA. Globular embryos could not acquire desiccation tolerance, even if they were treated with ABA. Though desiccation tolerance of the embryos was also induced by high osmoticum, the frequency of plant conversion (1.3-8.3%) was lower than that treated with ABA. The dry embryos maintained their plant conversion ability after six months of storage under room temperature conditions. The plantlets recovered from dry embryos were not morphologically different from those from non-desiccated ones.SDS-PAGE and western blot analysis of proteins revealed that the embryos treated with ABA accumulated seed storage proteins. And two dimensional gel analysis of the embryo proteins indicated that several new polypeptides were synthesized during ABA treatment and they may be associated with the acquisition of desiccation tolerance. A cDNA library representing mRNA's present in embryos treated with ABA was screened using Lea gene (pLEA76) as a probe. Six cDNA clones were selected. Now we are characterizing these cDNA clones. Less
人工种子技术可以在植物繁殖系统中提供潜在的益处。如果体细胞胚在含水量降低到真种子水平后仍能保持活力,则可作为种质保存的方法、植物发育生物学研究的模式系统和人工种子的利用。本研究旨在探讨外源阿坝对芸苔属作物小孢子胚脱水耐性的诱导作用,并对脱水耐性胚的蛋白质和基因进行分析。阿坝诱导胚的脱水耐性。这些现象受胚的大小和阿坝浓度的影响,但不依赖于基因型。用10^-10 ^M阿坝处理干子叶胚,转化率<-4><-5>为41- 53%。相比之下, ...更多信息 所有的胚在干燥后,当它们没有用阿坝处理时,都失去了它们的生活力。球形胚即使经过阿坝处理也不能获得脱水耐性。高浓度拟南芥也能诱导胚的脱水耐性,但转化率(1.3-8.3%)低于阿坝处理。干胚在室温条件下储存6个月后保持其植物转化能力。经ABA处理的胚在形态上与未处理胚无明显差异,蛋白质的SDS-PAGE和westernblot分析表明,阿坝处理的胚积累了种子贮藏蛋白。对胚蛋白质的双向电泳分析表明,阿坝处理过程中合成了几种新的多肽,它们可能与脱水耐性的获得有关。以莱亚基因(pLEA 76)为探针,筛选了阿坝处理的胚中存在的mRNA的cDNA文库。选择了6个cDNA克隆。现在我们正在鉴定这些cDNA克隆。少

项目成果

期刊论文数量(7)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
高畑 義人: "ブロッコリ-(Brassica oleracea L.)花粉由来胚の乾燥抵抗性" 育種学雑誌. 40巻別冊2. 92-93 (1990)
Yoshito Takahata:“西兰花 (Brassica oleracea L.) 花粉衍生胚的抗旱性”,《育种杂志》第 40 卷,特刊 2. 92-93 (1990)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
和久井 健司: "白菜小胞子由来胚の乾燥耐性に及ぼす浸透圧の影響および乾燥耐性胚のタンパク質の電気泳動分析" 育種学雑誌. 45巻別冊1. (1993)
Kenji Wakui:“渗透压对大白菜小孢子胚胎耐干燥性的影响以及耐干燥胚胎中蛋白质的电泳分析”,《育种杂志》第 45 卷,增刊 1。(1993 年)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Takahata, Y., K. Wakui and N. Kaizuma: "Induction of desiccation tolerance in Chinese cabbage microspore-derived embryo by ABA." Japan. J. Breed.41(Supp1.2). 266-267 (1991)
Takahata, Y.、K. Wakui 和 N. Kaizuma:“通过 ABA 诱导大白菜小孢子衍生胚的干燥耐受性。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
高畑 義人: "ABAによる白菜花粉由来胚の乾燥耐性の獲得" 育種学雑誌. 41巻別冊2. 266-267 (1991)
Yoshito Takahata:“通过 ABA 获得大白菜花粉衍生胚胎的干燥耐受性”,《育种杂志》第 41 卷,特刊 2. 266-267 (1991)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

TAKAHATA Yoshihito其他文献

TAKAHATA Yoshihito的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('TAKAHATA Yoshihito', 18)}}的其他基金

Identification and functional analysis of genes related with microspore embryogenesis
小孢子胚胎发生相关基因的鉴定及功能分析
  • 批准号:
    18380003
  • 财政年份:
    2006
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Analysis of microspore embryogenesis by exhaustive isolation of cDNA and development of useful tool collecting embryogenic microspores
通过详尽分离 cDNA 分析小孢子胚胎发生并开发收集胚胎发生小孢子的有用工具
  • 批准号:
    14360001
  • 财政年份:
    2002
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Analysis of induction mechanism of desiccationtolerance of embryos and production of osmotic tolerantplants
胚胎耐干燥诱导机制分析及耐渗透植物生产
  • 批准号:
    10660004
  • 财政年份:
    1998
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of plant breeding technology and new traits of Brassica crops using microspore culture
小孢子培养育种技术及芸苔属作物新性状开发
  • 批准号:
    07556002
  • 财政年份:
    1995
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Mechanism of desiccation tolerance of somatic embryos and application of dry artificial seeds
体细胞胚耐干燥机制及干燥人工种子的应用
  • 批准号:
    06660003
  • 财政年份:
    1994
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

Confocal laser microscopic observation of the effects of culture environment on somatic embryo formation
共聚焦激光显微观察培养环境对体细胞胚胎形成的影响
  • 批准号:
    10460114
  • 财政年份:
    1998
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of an automated somatic embryo production system equipped with image analysis facilities
开发配备图像分析设施的自动化体细胞胚胎生产系统
  • 批准号:
    03556036
  • 财政年份:
    1991
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了