Development of an automated somatic embryo production system equipped with image analysis facilities

该研究的目的是开发一种自动化的体细胞胚胎生产系统，在该系统中，选择优质的鱼雷胚胎进行移植或人工种子生产的进一步处理。根据每个体细胞胚的图像分析进行选择。本研究由三部分组成。胚性愈伤组织自动选择系统的研制。在常规的人工体细胞胚生产方法中，通过三种不同大小的筛孔筛选胚性愈伤组织。这个过程是自动化的，并使用胡萝卜愈伤组织进行了测试。结果表明，机械筛分增加了畸形胚的比例，但自动化是可能的。体细胞胚胎图像分析方法的发展。开发并测试了用于选择高质量鱼雷级胚胎的图像分析算法。以胡萝卜体细胞胚图像为例，比较了基于稀疏、广义霍夫变换和傅立叶描述子的算法。结果表明，该算法具有较好的应用前景，其结果与人类判断的一致性达到90%。体细胞胚胎分选器（soms）的发育。构建并测试了胚胎筛选系统。实现了细化算法。根据每个胚胎的图像分析结果，改变含胚胎液体培养基的路径。结果显示了some的有效性，但同时在成像细胞中运动胚胎的图像分析方面存在困难。分析了产生误判的原因，提出了成像单元采用较细的摄像管，从两个角度获取图像的改进意见。正在朝着这个方向努力改进某些系统。

The objective of the study was to develop an automated somatic embryo production system, in which good quality torpedo stage embryos were selected for further treatment of transplant or artificial seed production. The selection was executed based on image analysis of each somatic embryo. The study consisted of three parts.1. Development of an automated embryogenic callus selection system. In a conventional manual method of somatic embryo production, embryogenic callus was selected by sieving with three different size mesh. This procedure was automated and tested using carrot callus. The results showed increased ratio of malformed embryos by mechanical sieving, but that the automation was possible.2. Development of image analysis method of somatic embryos. Image analysis algorithms for selecting quality torpedo stage embryos were developed and tested. Algorithms based on thinning, generalized Hough transform, and Fourier descriptor were compared using carrot somatic embryo images. The results showed that the thinning algorithm was most promising which gave results 90% in agreement with human judgments.3. Development of SOMES (SOMatic Embryo Sorter). A system for selecting embryos was constructed and tested. The thinning algorithm was implemented. Path of liquid medium containing embryos was changed according to the results of image analysis of each embryo. The results showed the effectiveness of SOMES, but at the same time difficulties in image analysis of moving embryos in the imaging cell. The reasons of misjudgments were analyzed and points to be improved were suggested : use of a thinner tube for the imaging cell, and acquiring images from two angles. Efforts are being made to improve SOMES in this direction.