Molecular biological characterization and hypoallergenicity of seafood allergens

海鲜过敏原的分子生物学特征和低过敏性

基本信息

项目摘要

1.Parvalbumin(PA) was purified from nine species of fish and the edible frog and demonstrated to be a major allergen in all species. Primary structures of PA from eight species of fish were elucidated by a cDNA cloning technique. Recombinant mackerel PA expressed in E. coli was shown to be comparable in IgE binding ability to native PA.2.Collagens from various species of fish were confirmed to be allergenic. In the case of rainbow trout and mackerel, a chains separated were shown to be all allergenic and cross-reactive. Five overlapping proteins that cover the entire sequence of rainbow trout α2 chain were individually expressed in E. coli and the recombinant protein corresponding to the C-terminal region of the α2 chain was found to contain important IgE binding epitopes.3.Tropomyosin(Tm) was established to be a major and cross-reactive allergen in common with various species of crustaceans and cephalopods. Primary structures of Tm from five species of crustaceans and five species of cephalopods were determined by cDNA cloning.4.Monoclonal and polyclonal antibodies against crustacean and molluscan Tm were produced and shown to be applicable to specific analysis of crustacean Tm by sandwich ELISA. The surface plasmon resonance method using monoclonal antibodies raised against fish PA was useful to determine PA in seafood with high sensitivity.5.Allergen-responsive Th1 cells were obtained from BALB/c mice immunized with either mackerel PA or American lobster Tm. Analysis of the Th1 cell response to synthetic overlapping peptides revealed that T cell epitopes of mackerel PA are contained in peptides 11-25,26-40 and 56-75 and those of American lobster Tm in peptides 146-160,196-210 and 226-250.
1.从9种鱼类和食用蛙中分离纯化了小清蛋白(Parvalbumin,PA),PA是9种鱼类和食用蛙的主要过敏原。利用cDNA克隆技术对8种鱼类的PA进行了一级结构分析。重组鲭鱼PA在E.大肠杆菌的IgE结合能力与天然PA相当。2.多种鱼类胶原蛋白具有致敏性。在虹鳟鱼和鲭鱼的情况下,分离的链被证明是所有过敏和交叉反应。覆盖虹鳟鱼α2链整个序列的五种重叠蛋白在大肠杆菌中单独表达。原肌球蛋白(Tropomyosin,Tm)是甲壳类动物和头足类动物常见的主要过敏原。利用cDNA克隆技术对5种甲壳类和5种头足类Tm的一级结构进行了分析。4.制备了甲壳类和软体类Tm的单克隆抗体和多克隆抗体,并应用夹心ELISA法对甲壳类Tm进行了特异性分析。5.用鲭鱼PA和美洲龙虾Tm免疫BALB/c小鼠,获得过敏原应答性Th 1细胞。Th 1细胞对合成的重叠肽的反应分析显示,鲭鱼PA的T细胞表位包含在肽11- 25、26 -40和56-75中,而美洲龙虾Tm的T细胞表位包含在肽146- 160、196 -210和226-250中。

项目成果

期刊论文数量(58)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Proteome analysis reveals caspase activation in hyporesponsive CD4 T lymphocytes induced in vivo by the oral administration of antigen
蛋白质组分析揭示口服抗原在体内诱导的低反应性 CD4 T 淋巴细胞中的半胱天冬酶激活
Expression and evaluation of IgE‐binding capacity of recombinant Pacific mackerel parvalbumin
  • DOI:
    10.1111/j.1440-1592.2004.00344.x
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    6.8
  • 作者:
    Y. Hamada;Hiroyuki Tanaka;Ayako Sato;S. Ishizaki;Y. Nagashima;K. Shiomi
  • 通讯作者:
    Y. Hamada;Hiroyuki Tanaka;Ayako Sato;S. Ishizaki;Y. Nagashima;K. Shiomi
Reactivity of IgE in fish-allergic patients to fish muscle collagen
鱼过敏患者 IgE 对鱼肌肉胶原蛋白的反应性
  • DOI:
  • 发表时间:
    2003
  • 期刊:
  • 影响因子:
    0
  • 作者:
    T.Noguchi;O.Arakawa;T.Takatani;Yuki Hamada
  • 通讯作者:
    Yuki Hamada
Oral administration of food antigen induces T cell mediated intestinal inflammation : A model using TCR-transgenic mice.
口服食物抗原诱导 T 细胞介导的肠道炎症:使用 TCR 转基因小鼠的模型。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Kikuchi A;Nakajima-Adachi H;Ebihara A;Takahashi Y;Hosono A;Itoh K;Hachimura S;Kaminogawa S
  • 通讯作者:
    Kaminogawa S
Purification and molecular cloning of a major allergen from Anisakis simplex.
单纯异尖线虫主要过敏原的纯化和分子克隆。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    K.Shimakura;H.Miura;K.Ikeda;S.Ishizaki;Y.Nagashima;T.Shirai;S.Kasuya;K.Shiomi
  • 通讯作者:
    K.Shiomi
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SHIOMI Kazuo其他文献

SHIOMI Kazuo的其他文献

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{{ truncateString('SHIOMI Kazuo', 18)}}的其他基金

Elucidation of epitopes of seafood allergens
海鲜过敏原表位的阐明
  • 批准号:
    19380120
  • 财政年份:
    2007
  • 资助金额:
    $ 33.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Studies on allergens in fish and their processed products
鱼类及其加工品中过敏原的研究
  • 批准号:
    11460097
  • 财政年份:
    1999
  • 资助金额:
    $ 33.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Screening, structure and usefulness of marine bioactive proteins
海洋生物活性蛋白的筛选、结构和用途
  • 批准号:
    08306011
  • 财政年份:
    1996
  • 资助金额:
    $ 33.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Studies on proteinaceous toxins newly found in bivalves belonging to the genus Corbicula
蚬属双壳类新发现的蛋白质毒素研究
  • 批准号:
    07660267
  • 财政年份:
    1995
  • 资助金额:
    $ 33.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Biochemical studies on proteinaceous toxins in the skin mucus and roe of the lamprey Lampetra japonica
七鳃鳗皮肤粘液和鱼子中蛋白质毒素的生化研究
  • 批准号:
    04660220
  • 财政年份:
    1992
  • 资助金额:
    $ 33.28万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Studies on biologically active proteinaceous substances contained in the skin mucus of the Japanese eel
日本鳗鱼皮肤粘液中生物活性蛋白质物质的研究
  • 批准号:
    02660211
  • 财政年份:
    1990
  • 资助金额:
    $ 33.28万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Biochemical studies on fish venoms
鱼毒的生化研究
  • 批准号:
    63560202
  • 财政年份:
    1988
  • 资助金额:
    $ 33.28万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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Gap resolution in safety, nutritional, allergenicity and environmental assessments to promote alternative protein utilization and the dietary shift.
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    2022
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磷酸基团对酪蛋白成分致敏性的影响:提高牛奶过敏的诊断准确性。
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Establishment of methods of prevention for anisakis allergy by reducing allergenicity
通过降低过敏原性来建立异尖线虫过敏的预防方法
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尝试使用育种和染色体操作技术来降低鲑鱼肉和器官的鱼子过敏性
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    2020
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不同加工方法加工的小麦过敏原致敏性差异分析
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评估古代和现代小麦的麸质过敏性,以制定减少小麦麸质蛋白中引起乳糜泻的策略
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    513705-2017
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对桦树花粉产生和过敏性的生物和非生物影响以及相关的健康影响
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评估古代和现代小麦的麸质过敏性,以制定减少小麦麸质蛋白中引起乳糜泻的策略
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使用原花青素评价小麦的降低过敏性
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