Integration of the various receptor transmitter systems in the central nervous system -with special reference to the receptor autoradiography

中枢神经系统中各种受体递质系统的整合 - 特别参考受体放射自显影

• 批准号: 02670027
• 负责人: NOMURA Takako
• 金额: $ 1.41万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670027/
• 关键词: Receptor; Autoradiography; Muscarinic Cholinergic Receptor; Hemi-parkinsonian Rats; Dopamine receptor; Ischemia; GABAReceptors; 水迷路減試験; 水迷路試験; ヘミ・パ-キンソン病モデル; ド-パミン レセプタ-

We established the procedures of receptor in vitro macro autoradiographical methods for neurotransmitter system in the central nervous system. By using these methods, we investigated the hemi-parkinsonian rats which were induced by the administration of 6-OHDA in the left substantia nigra. In the stratum of the hemi-parkisonian rats, dopaminergic receptors showed the up-regulation and dopamine uptake sites were completely disappeared. On the other hand, we could not find out the alteration of the cholinergic receptors concentration.Administration of ibotenic acid to the Meynert nucleus, we induced expeimental dementia rats. In these rats, the cholinergic receptors in the cerebral cotex were disapperared.The experimental ischemic rats were made by occulusion of the mid cerebral artery for 1 hour. We investigated the GABAnergic receptors in the ischemic rats brain using as ligands, 3H-labeled muscimol and SR 95531, which are considered much more specific binding ligand for GABA A receptor. As a results, GABAnergic receptors in the ischemic regions were significantly and simutanenously decreased.

建立了中枢神经系统神经递质系统受体体外宏观放射自显影方法。利用上述方法，我们观察了6-OHDA诱发的偏侧帕金森病大鼠的左侧黑质的变化。在偏侧帕金森病大鼠皮层，多巴胺能受体表达上调，多巴胺摄取部位完全消失。另一方面，我们没有发现胆碱能受体浓度的变化。鹅膏蕈氨酸给药于Meynert核，我们诱导实验性痴呆大鼠。用大脑中动脉阻断1小时造成实验性脑缺血大鼠。用3 H标记蝇蕈醇和SR 95531作为GABAA受体的特异性结合配体，研究了缺血大鼠脑内GABA能受体的变化。结果表明，缺血区GABA能受体明显减少。

项目成果

Demura,N.et al: "Acute cerebral ischemia model produced by injection of microspheres via external carotid artery: behavioral,histopathological and autoradiographical studies in freeーmoving rats" Brain Research.

Demura, N. 等人：“通过颈外动脉注射微球产生的急性脑缺血模型：自由活动大鼠的行为、组织病理学和放射自显影研究”大脑研究。

Demura,N.et al.: "Acute cerebral ischemia model produced by injection of microspheres via external carotid artery:behavioral,histopathological and autoradiographical studies in free-moving" Brain Research.

Demura,N.等人：“通过颈外动脉注射微球产生的急性脑缺血模型：自由活动中的行为、组织病理学和放射自显影研究”大脑研究。

Mizukawa.K.et al.: "Changes of phorbol ester binding sites in rat brain following intracerebroventricular administration of thyrotropinーreleasing hormone (TRH):an in vitro macroautoradiographic investigation" Neurochemical Research. 17. 361-365 (1992)

Mizukawa.K.等人：“脑室内施用促甲状腺素释放激素（TRH）后大鼠脑中佛波酯结合位点的变化：体外宏观放射自显影研究”《神经化学研究》17. 361-365（1992）。

水川 公直.他: "〔脳と運動のレセプタ-〕 黒質細胞移植による運動機能の修復とド-パミン産生およびレセプタ-の変化" 平凡社, 242.(211-224) (1990)

Kiminao Mizukawa 等人：“[大脑和运动受体]通过黑质细胞移植恢复运动功能以及多巴胺产生和受体的变化” Heibonsha，242.(211-224) (1990)

Ogawa,N.,et al: "Postーischemic administration of bifemelane hydrochloride prohibits ischemiaーinduced depletion of the muscarinic M1ーreceptor and its mRNA in the gerbil hippocampus" Brain Research.

Okawa, N. 等人：“缺血后给予盐酸比非美拉恩可抑制沙鼠海马中缺血诱导的毒蕈碱 M1 受体及其 mRNA 的消耗”《大脑研究》。